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Object of Study and Methods We obtained a Chinese pedigree with bilateral congenital microtia of concha type in 56 members of 5 generations family,we analyzed their clinical and hereditary features,epidemiology database and blood sample were collected for further study as well.

结果：该患者家系内共有成员56人，无近亲婚配情况，共有5代发病，累及患者20名，均为先天性双侧耳甲腔型小耳畸形患者，未伴有Treacher-Collins综合征及Millers综合征的相关表现，未发现心血管系统，泌尿系统以及四肢等发育畸形。

Results: Different manifestations of skin infection, such as nodule, papule, ulcer and lesions mimicking molluscum contagiosum appeared 2-9 d after inoculation, with a mean of (3.42±1.17) d in immunocompromised group and (4.25±1.42) d in immunocompetent group.

结果：两组小鼠均于接种菌悬液后2~9 d［免疫抑制组(3.42±1.17)d、未抑制组(4.25±1.42)d］产生了结节、丘疹、溃疡、传染胜软疣样皮损，并均于接种后22~44 d［免疫抑制组(36.75±4.20)d、未抑制组(29.00±4.75)d］自愈。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Four different ionic liquids, BF4, BF and PF6 were investigated, arid PF6 showed the best effect when used as the reaction media, giving the highest yield in the shortest reaction time. Promoted by KF in PF6, N-aryl or N-alkyl monosubstituted sulfonamides could react with various activated halides and unactivated primary halides smoothly. Reactions were performed at 85℃ for 2~10 hours, and thirteen N-alkylation products were obtained with 70%~97% yields. Moreover, the ionic liquid recovered could be reused for three times with no appreciable decrease in yield and reaction rate.

结果表明，以PF6为反应介质时显示出了最好的效果，反应的时间最短，收率最高。N-芳基或N-烷基单取代的磺酰胺和各类不同结构的活化的卤代烃和未活化的一级卤代烃在PF6中KF作用下，85℃反应2~10h，以70%~97%的优良收率得到了13个N-烷基化产物，而且离子液体回收使用3次后效果未降低。

In all cases, distal spread was noted into the cleavage plane between the multifidus and longissimus muscles. In no instance did the 0.5 ml of contrast fail to bathe the target.

在所有情况中，注射液向远处播散仅见于多裂肌和最长肌的间隙中。0.5ml注射液未浸润注射点的情况未见发生。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件，消融人肝细胞癌株SMMC-7721细胞悬液，分为五组：A、对照组，B、45℃10min组，C、50℃3min组，D、54℃3min组，E、60℃即刻组，消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤，间隔1周，右侧腹股沟皮下接种瘤块作为观察瘤，成瘤后分为四组：A组，对照组，不行治疗；B组，微波组，仅微波消融小鼠消融瘤；C组，成熟DC组，微波消融小鼠消融瘤，并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞；D组，未成熟DC组，微波消融小鼠消融瘤，并于消融瘤周注射未成熟树突状细胞。

Though the molecular mass and the hypoglycemic activity of myristic acid modified HIDesB30 are both consistent with Detemir, the expression system, purification artwork and the methods of modification were different from Detemir.

本研究实现了含短C肽的人胰岛素原类似物HMPIDesB30在毕赤酵母中的高效分泌表达，表达量达1 g/L，分泌表达的重组蛋白HMPIDesB30未出现糖基化，未形成二聚体和多聚体，并具有生物学活性。

Citizen Chinese people to get the prize. But they has a blood tie with China have only, can't truly represent the development level of Chinese and technology, the Chinese mainland did not carry out the Nobel prize "zero of breakthrough", see the motherland increasingly strong, have already become the necessar y and important role in the stage of world.

虽获奖中有7位美籍华人，但他们与中国仅有血缘关系，中国本土至今未实现诺贝尔奖&零的突破&，中国已日益强大，成为世界舞台上不可或缺的重要角色，而在诺贝尔奖的领奖台上，却始终未出现中国人的身影。

The remainder of the priests, Levites, including the Nethinim (who were non-Israelite helpers of the Levites), and all others who had obeyed the Lord in following Ezra's reform in separating themselves from their heathen wives and children (Ezra 10:3), and those who had spiritual understanding and knowledge (10:28-29, key verse), all verbally and publicly affirmed the oath,"to observe and do all the commandments of the Lord"(10:29b).

其余的祭司、利未人（包括尼提宁人，他们是协助利未人的非以色列殿役）、追随以斯拉的改革而服从主，离弃外邦妻儿的（拉10：3），凡有知识能明白的人（10：28，29钥节），皆以口公开宣誓「必遵行上帝……的律法」（10：29下）。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。