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Object To explore the method for high quality DNA extract from plant Chinese medicinal materials containing developed xylem Methods The process of DNA includes wash with Tris buffer, extract with CYAB buffer and purification mainly Results Using this method, the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L., Paeoniae lactiflora Pall.,Aucklandia lappa Decne.,Lindera aggregata Kostern., and Akebia quinata Decne.

目的 为获得高质量的DNA,研究木质部较发达的根,根茎类以及茎木类药材的DNA提取方法。方法总DNA的提取过程包括用Tris缓冲液洗净,CTAB抽提和纯化。

The leafstalk, chief vein, bud, the cane top on side branch, bast and xylem of type B had type A and C tow components, belonging to chimeric tissues. The leaf pulp and thin leaf vein had no components of type C, belonging to pure tissues of type A. Type B plant had two kinds of color roots, the rufous roots belonged to type A tissue, the yellow roots belonged to type C tissue through the SSR identification.

深州杨B型的叶柄、叶主脉、芽、侧枝茎尖、韧皮部、木质部同时检测到深州杨A、C型两种成分存在,属于嵌合组织,B型叶肉及细叶脉中检测不到深州杨C型成分,属于深州杨A型纯合组织;深州杨B型植株的地下部分含有红褐色和土黄色两种根,经检测,红褐色根是属于深州杨A型的纯合组织,土黄色根是属于深州杨C型的纯合组织。

The abnormality in parencyhyma among the vascular bundle, the parenchyma cells near periderm broken cleft, produced crannies and developed a group of deep color unusual tissues. As well as the disrepair phenomena often happened in the timber parenchyma cell near xylem.

3维管束间的薄壁组织异常,近周皮附近的薄壁细胞组织破裂产生裂隙,产生染色较深的一群异样组织,木质部附近的木薄壁细胞有破损现象。

The secondary phloem is larger than the secondary xylem in the Heracleum , which is the typical storing root .

根据独活属植物根的次生韧皮部远比次生木质部发达的特点,确定独活属植物的根是典型的贮藏型根。

Thin leaf section were prepared from fresh tissues of YS-lnfected and healthy citrus plants, when observed under a fluorescence microscope (Type, Olympus AH-FL-2), a yellow fluorescence was distinctly observed in the YS-infected phloem tissues and not in the phloem tissuses of healthy plants and of those infected with tristeza, exocortis, canker, scab, greasy...

将健叶和黄龙病叶叶柄用保险刀片徒手切片,在透射式荧光显微镜下检查,可看到健叶叶柄的木质部导管细胞壁发黄色荧光,韧皮纤维细胞发绿色荧光,而病叶叶柄韧皮部中有1~多个鲜明的黄色或黄绿色荧光团块。这种特异性的荧光,在健叶柄韧皮部中是没有的,其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦没有此种特异性荧光。

The rate of absorption and export by the leaf related closely to the exposure time with an asymetotic curve. The equations are: Y=93.0-84.8(0.987)~x and Y=86.0-79.1(0.986)~x,respectively.Autoradiograph of seedlings treated on a single leaf(dose:107.3×10~3Bq)showed that not only PP333 was transported acropetally to the tip, but also moved basipetally to the roots.

过去的研究工作所得的普遍结论是:PP333是通过木质部运输,叶片吸收的PP333向其他部位运输的量极少,因而,叶片吸收在抑制生长中不起或不起重要作用,即叶片吸收无效阁。

The tracheary elements in secondary xylems of Actaea asiatica root and rhizomes were observered with a scanning electron microscope and found to contain abundant types, which mainly included tracheids, tracheid-like vessels, fibriform vessels, and typical vessels. Of these types, the tracheids, tracheid-like vessels, fibriform vessels were first found. Scalariform perforation plates, reticulate perforation plates, mixed perforation plates and simple perforation plates were observed in the typical vessels and the reticulate perforation plates ,mixed perforation plates were first founded the lateral-wall perforation plates, multiple end walls and pit membrane vestige were also observed in the tracheary elements.

利用扫描电子显微镜对毛茛科类叶升麻根和根状茎次生木质部中的管状分子进行观察,发现其管状分子类型丰富,主要有:管胞、管胞状导管、纤维导管和典型的导管分子,其中管胞、管胞状导管和纤维导管为在该类群中首次报道;在导管分子中,存在着梯状穿孔板、网状穿孔板、混合型穿孔板和单穿孔板,其中网状穿孔板和混合型穿孔板为在该类群中的首次报道;对其导管分子上的侧壁穿孔板、多穿孔板和纹孔膜残余也进行了描述。

The primary studies on insecticidical and fungicidical components of endophytic fungus in Celastrus angulatus have been carried out from September, 2000 to June, 2002.The results are as follows: Taking PDA and Czapek as separating culture medium, 39 strains of endophytic fungus were separated from the bark, phloem and xylem of the root and twig of Celastrus angulatus.

作者于2000年9月至2002年6月对杀虫植物苦皮藤中内生真菌的杀虫杀菌活性物质进行了初步的研究,取得了以下结果:采用常规的内生真菌分离方法,以马铃薯葡萄糖培养基、Czapek培养基为分离用培养基,从苦皮藤根和侧枝的表皮、韧皮部和木质部中分离得到39株内生真菌。

The main characters of this category are: 1 It possesses clear main axes and lateral branches/sporangial stalks; 2 Sporangia terminate the lateral branches or long stalks; 3 With the distinct dehiscing mechanism, the sporangia have the variation in shape, i. e. reniform, rounded or vertically elongate; 4 Primary xylem is of centrarch maturation; 5 Conducting tracheid is G-type.

该类群包括Hsüa,Renalia,Nothia,Sartilmania和Huia,其主要特征为:1)具有明显的主茎轴及侧出的分枝系/孢子囊柄;2)孢子囊顶生于侧枝上或侧生的长柄上;3)孢子囊性状存在变异,为肾形、圆形或纵向伸长,具明显的开裂机制;4)初生木质部发生顺序为心始式;5)G-型输水管胞。

In functional study, overexpression assay was conducted in potato. Interestingly, tuberous swelling along roots was observed in some transgenic potato lines in addition to stem-derived tuber formation. Conclusively, we propose a hypothesis to illustrate the mechanism by which IbMADS1 participates in the initiation of tuberization in sweet potato.

利用解剖切片观察并比较转殖马铃薯与野生型甘薯的根部块状膨大区域,可以发现两者在膨大初期,中柱内含的细胞数皆增多,尤其是木质部细胞出现不正常增生情形,进而扩展中柱区域;因此,我们可以初步推论在甘薯块根发育初期,IbMADS1藉著作用於根部分生组织,尤其是中柱的原始形成层,从而促进细胞分裂,并参与甘薯的初期结薯。

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