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Compound enzymes of cellulose,pectolase and papain with ratio of 2 : 1 : 1 were used to isolate polysaccharides from laminaria,and influence of two different ways of addition of compound enzymes on polysaccharide extracting rate and polysaccharide content were studied.

采用复合酶法(纤维素酶、果胶酶、木瓜蛋白酶,比例为2:1:1)从海带中提取3种多糖,并分析了不同加酶方式对海带多糖提取率和含量的影响。

On the other hand, the amidase activity and esterase activity of papain were determined at different temperatures.

一方面,通过比较常温溶液体系、低温溶液体系以及低温固液体系中的肽合成产率,发现低温和固液共存是动力学控制肽键合成的两个有利因素:另一方面,通过对木瓜蛋白酶催化水解动力学的研究,分别测定了该酶在不同温度下的酯酶活性和酰胺酶活性。

398 Neutral protease,pepsin,papain and bromelin were utilized to hydrolyze the head of Penaeus monodon .

利用AS1.398中性蛋白酶、胃蛋白酶、菠萝蛋白酶及木瓜蛋白酶对斑节对虾头蛋白进行酶水解试验,从中选出了优选试验用酶,并在此基础上对优选酶的最适酶解工艺条件进行了探讨。

In Chapter 2 of this thesis, the kinetic equation of substrate reaction in presence of denaturant has been derived. A re-examination of the effect of high concentrations of guanidine hydrochloride on the inactivation of papain, taking into consideration the effect of high concentrations of guanidine hydrochloride on the Michaelis constant, showed that, for papain, the substrate gives no protection on its inactivation.

我们在第二章中推导了变性剂存在下的底物反应动力学方程,并在考虑了高浓度盐酸胍对酶催化反应米氏常数的影响的前提下,根据推导的动力学方程重新分析了盐酸胍存在下木瓜蛋白酶的失活动力学,结果表明底物对木瓜蛋白酶的失活没有保护作用。

The orthogonal test of removing protein with pawpaw proteinase analysed by difference demonstrates: the sequence of the ponderance affecting doffing protein from high to low is the treating temperature, enzyme quantity, the treating time and the pH value.

经木瓜蛋白酶正交试验极差分析,影响因素从大到小排列顺序依次为:温度、酶量、处理时间、 pH 值,最佳处理条件为温度50℃、酶用量为2%、酶解2h 、 pH 为6.0,又脱去剩余蛋白的80.2%,蛋白质浓度降到0.4mg/mL 。

Water-solubility and emulsibility of gluten were improved by the hydrolysis of Papain.

木瓜蛋白酶水解面筋蛋白可提高水溶性和改善乳化性能,适宜的水解条件为:酶解温度55℃、酶解pH值3.0、酶液浓度1.0%、底物浓度20%、水解时间3h~4h。

The results showed that the optimum enzymatic conditions were: choosing papain, with substrate concentration 16%, enzyme concentration 4000U/g, hydrolysis time 6h, and the optimum conditions for the enzymatic hydrolysate of porcine blood to synthesize nitrosyl heme-polypeptide were: NaNO2 added 1.6%, pH5.00 and reaction time to 12min at 55℃.

试验表明,以选用木瓜蛋白酶效果较好,最佳酶解条件为:底物浓度16%,加酶量4000U/g,水解时间6h;酶解液合成色素最佳参数为:pH5.00,反应时间12min ,反应温度为55℃,NaNO2 的添加量为1.6%。

The free radical scavenging effects of Meretrix meretrix hydrolysates prepared with papain,neutral protease and acid protease were investigated.

利用正交实验研究了文蛤蛋白分别经木瓜蛋白酶、中性蛋白酶、酸性蛋白酶作用后酶解物对羟自由基和超氧自由基的清除作用,优化了水解条件,并对最佳清除率下的三种酶解物进行SephadexG50凝胶柱分离,测定酶解物中抗氧化活性肽的分子量分布。

The composition and structure of Soybean Protein Isolate are first introduced,Then the functional modification of SPI via chemical ways which include the reaction of amino group,carboxyl group and sulphydryl group,via enzymic ways which include the modification using transglutaminase enzyme and papain,via physical ways which include blending and heat treatment are reviewed.

首先介绍了大豆分离蛋白的基本组成与结构,然后分别从化学改性、酶改性和物理改性三个方面对大豆分离蛋白改性进行了综述。其中,在化学改性方面,针对大豆分离蛋白中含有的氨基、羧基、巯基等不同活性基团的改性原理及研究现状进行了介绍。在酶改性方面,主要介绍了谷胺酰胺转胺酶、木瓜蛋白酶等对大豆分离蛋白的改性作用。在物理改性方面,介绍了共混、加热改性等目前研究较多的方法。

Papain was modified using different anhydrides. The average ratio of modified-NH2 was tested by a trinitrobenzenesulfonic acid method. The native and modified papain samples were purified by dialysis and their structures were characterized by UV and IR.

用不同酸酐对木瓜蛋白酶进行化学修饰,以三硝基苯磺酸法测定修饰酶的平均氨基修饰度,对修饰前后的木瓜蛋白酶分别纯化并通过UV-vis和IR对其结构进行了表征。

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