木瓜蛋白酶

The hydrolysis of soybean protein isolate with immobilized papain for making soy peptide was studied.

采用固定化木瓜蛋白酶水解大豆分离蛋白，对大豆分离蛋白的最佳预处理温度进行了探讨，并对制备大豆肽的工艺条件进行了正交实验。

Papain was successfully purificated by a simple method.

应用此方法成功纯化了高纯度的木瓜蛋白酶。

Purpose To construct a simple way for purifying papain from Carica papaya.

目的 建立木瓜蛋白酶的分离纯化工艺路径。

Calcium chloride ; ginger juice ; papain ; beef ; tenderize

氯化钙；生姜汁；木瓜蛋白酶；牛肉；嫩化

Methods Using means of collection, 20% and 40% saturated ammonium sulfate fractionation, SP-sephadex C 50 column chromatography and hydroxyapatite chromatography to purify papain from Carica papaya.

采用初步采集处理，20%，40%硫酸铵分级沉淀、SP-sephadex、C50柱色谱、羟基磷灰石柱色谱，从番木瓜乳汁中分离纯化木瓜蛋白酶。

The effect of the medicine wine Gu Yong KangNing to the osteoarthritis that was induced by the caroid.

永康宁骨康宁药酒对木瓜蛋白酶诱发膝骨性关节炎的影响。

There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液餵食饲养，於治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

Methods 24 rabbits were randomly divided into control and treatment groups. There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

The re were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

There were 12 rabbits (24 articular genus) in every group. Osteoarthritis model was established by injection of caroid in articular genu intracavitary. Rabbits with osteoarthritis were interfered by Duhuojisheng decoction. The expression of IL-1 and TNF from synovial fluid in rabbit with osteoarthritis were measured by double antibody sandwich ELISA 1, 3, 5 weeks after treatment.

将24只兔随机分为2组，即对照组和治疗组，各12只，24个膝关节，采用兔膝关节腔内注入木瓜蛋白酶造成骨关节炎的病理模型，以独活寄生汤浓缩液喂食饲养，于治疗后1、3、5 周分别抽取关节液，采用双抗体夹心酶联免疫吸附法，分别测定兔膝关节液中IL-1及TNF 表达水平。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。