木瓜蛋白酶

The enzymolysis effect of neutral protease, papain, pepsin, bromelin, flavourzyme and compound protease was studied respectively on the sleeve-fish protein.

探讨了中性蛋白酶、木瓜蛋白酶、胃蛋白酶、风味蛋白、酶菠萝蛋白酶以及复合蛋白酶对鱿鱼蛋白的酶解效果。

Oyster was hydrolysed with six kinds of commercial proteinases including pepsin, trypsin, bromelin, papain, 3942 neutral proteinase and Alcalase 2.4L. Three enzymes of better hydrolysis effect were chosen as the target enzymes, taking the α-amino nitrogen in the hydrolysates as the index. The hydrolysis conditions of the three enzymes were investigated by single factor experiment and orthogonal test.

选取胃蛋白酶、胰蛋白酶、菠萝蛋白酶、木瓜蛋白酶、3942中性蛋白酶、Alcalase碱性蛋白酶等对牡蛎进行水解，以水解液中氨基酸态氮的含量为指标，确定了3种水解效果较好的酶：Alcalase碱性蛋白酶、胰蛋白酶和菠萝蛋白酶。

In Chapter 2 of this thesis, the kinetic equation of substrate reaction in presence of denaturant has been derived. A re-examination of the effect of high concentrations of guanidine hydrochloride on the inactivation of papain, taking into consideration the effect of high concentrations of guanidine hydrochloride on the Michaelis constant, showed that, for papain, the substrate gives no protection on its inactivation.

我们在第二章中推导了变性剂存在下的底物反应动力学方程，并在考虑了高浓度盐酸胍对酶催化反应米氏常数的影响的前提下，根据推导的动力学方程重新分析了盐酸胍存在下木瓜蛋白酶的失活动力学，结果表明底物对木瓜蛋白酶的失活没有保护作用。

Using papain to hydrolyze the legumin and vicilin respectively showed that it could obtain higher DH from legumin than from vicilin, and the emulsifying activity of legumin hydrolysates was also increased more than vicilin hydrolysates. It was easier to hydrolyze the β-sheet fractions than α-helical fractions of broad bean legumin by papain, but to vicilin the β-sheet fractions was difficult to be hydrolyzed.

发现Papain对11S的水解程度大于7S，11S水解物的乳化活性也高于7S水解物；对水解物的圆二色性分析表明：蚕豆11S球蛋白的β-折叠结构易于被木瓜蛋白酶作用，而7S球蛋白的α-螺旋结构易于被木瓜蛋白酶作用；蛋白质分子中的α-螺旋结构对乳状液的形成和稳定起着重要作用。

Are specializing in the production of papaya powder, papain and bromelain of the production enterprises.

美仑生物制品有限公司是专业生产木瓜粉、木瓜蛋白酶和菠萝蛋白酶的生产企业。

The process of preparing of collagen peptide from pig skin by papain was studied.

以新鲜猪皮为原料，利用木瓜蛋白酶水解法制备胶原多肽，并对影响木瓜蛋白酶水解过程的各个因素进行研究。

It revealed high inhibition specificity toward serine proteinases (bovine trypsin, bovine chymotrypsin, and porcine trypsin). It did not exert any inhibitory activity toward cystein proteases and aspartyl proteinases.

丝氨酸蛋白酶抑制剂对丝氨酸蛋白酶家族中的牛胰凝乳蛋白酶、牛胰蛋白酶、猪胰蛋白酶三种蛋白酶的活性均有强烈的抑制作用；而对半胱氨酸蛋白酶家族中的木瓜蛋白酶及天冬氨酸蛋白酶家族中的牛胃蛋白酶几乎没有作用。

The experimental results revealed that papain, calcium chloride, compound phosphate, and glucide keeping humidity reagents intensify the tenderness of pork jerky, but the addition should be proper. The best proportion was papain0.4‰, compound phosphate 0.4%, calcium chloride 0.1%, and sorbitol 1.5%.

实验结果表明：木瓜蛋白酶、复合多聚磷酸盐、氯化钙和糖类保湿剂都可增加肉脯的嫩度，但用量应适当，添加量过大，反而使肉脯的感官品质变差；肉脯嫩化的最佳配比为木瓜蛋白酶0.4‰、复合多聚磷酸盐0.4%、氯化钙0.1%、山梨糖醇1.5%。

The coagulating effect of the two proteinases and their mixtureon 7S and 11S was compared. Results indicated that two proteinases interacted witheach other during the coagulating process. 11S gel induced by mixture of twoproteinases was more elastic, and 7S gel induced by separated proteinase was moreelastic.

然后采用SP-sepharose阳离子交换剂从木瓜蛋白酶分离出2种蛋白酶组分，对比它们单独作用和复合作用于7S和11S蛋白的促凝能力，结果表明木瓜蛋白酶中两种组分对7S和11S蛋白的促凝过程中存在协同作用，复合作用可以促使11S蛋白形成弹性更强的凝胶，而单独作用可以促使7S蛋白形成弹性更强的凝胶。

Papain was modified using different anhydrides. The average ratio of modified-NH2 was tested by a trinitrobenzenesulfonic acid method. The native and modified papain samples were purified by dialysis and their structures were characterized by UV and IR.

用不同酸酐对木瓜蛋白酶进行化学修饰，以三硝基苯磺酸法测定修饰酶的平均氨基修饰度，对修饰前后的木瓜蛋白酶分别纯化并通过UV-vis和IR对其结构进行了表征。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。