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The results of UPGMA duster analysis based on RAPD and ISSR fingerprint had the approximate trend, but not the same.(5) The synthetical comparison of RAPD and ISSR sustained the independency of Liriodendron, Manglietia, Parakmeria and Tsoongiodendron, and advised to redefine the range of Magnolia, subgen. Yulania indepent from Magnolia, then established Yulania, and abolished the division of subgenera and section Michelia, and set up Michelia.

基于RAPD标记和ISSR标记构建的树图,两者趋势大致相近,但不完全相同;(5)综合木兰科6属20种植物的RAPD和ISSR分析结果,支持鹅掌楸属、木莲属、拟单性木兰属和观光木属的独立;建议重新界定木兰属的范围,并成立玉兰属;建议取消含笑属下亚属和组的划分,设为含笑属。

In the leaves of Liriodendroideae, some of the abaxial epidermal cells are papillose and the vascular tissue of the main vein appears to be separated. However, papillose were not found and there are uniseriate, multicellular or unicellular hairs distributed on the epidermis, and the vascular tissue of the main vein appears to be continuous in the leaves of Magnolioideae. Furthermore, in the Magnolioideae, the structure of the leaves of plants in Manglietia are different from that of Magnolia.

结果表明:鹅掌楸亚科和木兰亚科在叶的结构上的主要区别是鹅掌楸亚科两种植物叶的部分下表皮细胞呈乳突状,且整个细胞外壁只形成一个乳突,而在木兰亚科的植物中有单列多细胞或单细胞的表皮毛,未发现乳突;鹅掌楸亚科植物的叶主脉维管组织环分隔呈束状,且其外包被的纤维也排列成束状,而木兰亚科的80种1亚种植物中,叶主脉维管组织连成轮状,其外面也由一圈连续的纤维环所包围。

However, the taxa of strictly tropical distribution are still underrepresented in the flora of southeastern Yunnan compared to Indo-Malaysian flora, and the families of mainly subtropical to temperate distribution, such as Magnoliaceae, Theaceae, Cornaceae, Styracaceae, Symplocaceae, Aquifoliaceae and Caprifoliaceae, are well represented in the flora. Some characteristic families of temperate East Asia, such as Diapensiaceae, Dipentodontaceae, Eupteleaceae, Grossulariaceae and Toricelliaceae are also present in the flora of southeastern Yunnan.

由於位於东南亚热带北缘山地及其地质历史原因,云南东南部的热带植物区系中严格热带分布的成分不多,仍以边缘热带成分为主,并有相对丰富的亚热带-温带科如木兰科、山茶科、山茱萸科、山矾科、忍冬科、冬青科等以及一些东亚和喜马拉雅特徵科,如岩梅科、十齿花科、领春木科、茶藨子科和鞘柄木科,显示了它与东亚温带植物区系也有较多联系。

Results of sampling showed that the typical evergreen broad-leaved forest of Mt. Jiulianshan consisted mainly of subtropical families and genera with dominant families including Fagaceae, Lauraceae, Camelliaceae, Styracaceae, Ericaceae, and Magnoliaceae.

群落主要由热带、亚热带科属组成,优势科为壳斗科Fagaceae,樟科Lauraceae,山茶科Camelliaceae,安息香科Styracaceae,杜鹃花科Ericaceae和木兰科Magnoliaceae等科,优势树种以相应的优势科属树种为主。

Mainly Lauraceae, Fagaceae, Theaceae, magnolia Branch, Division of Hamamelidaceae evergreen broad-leaved trees such as the composition of the shrub layer are found to evergreen species, community structure is more complex.

主要由樟科、壳斗科、山茶科、木兰科、金缕梅科等科的常绿阔叶树组成,灌木层中亦多常绿种类,群落结构比较复杂。

New species of Magnoliaceae of Yunnan.

标题 云南木兰科的新种。

Many species of Magnoliaceae were widely distributed in the Northern Hemisphere, such as Asia, Europe and North America while there was no fossil record in Africa and Oceania since Cretaceous.

自白垩纪以来,木兰科的许多种广泛发生于北半球,如亚洲、欧洲及北美等地,但非洲和大洋洲至今尚未发现木兰科的化石记录。

New species of Magnoliaceae of Yunnan.

Title 云南木兰科的新种。

The results showed as follow:(1) The method of improved CTABⅠequal to extract Magnoliaceae genomic DNA was developed, because the extracted DNA by the method was suitable for RAPD and ISSR analysis.(2) Single factor design combing uniform design was used to obtain optimum scheme on reaction system for RAPD and ISSR of Magnoliaceae.

研究结果如下:(1)建立了适合于木兰科6属20种植物DNA提取的改良CTAB法Ⅰ,用该法提取的DNA能满足RAPD和ISSR分析的要求;(2)采用单因素结合均匀试验设计优化得到适合于木兰科6属20种植物RAPD和ISSR分析的PCR体系。

Randomly amplified polymorphic DNA and inter-simple sequence repeat markers were used to analyse the genetic diversity and relationships of 20 species in 6 genera from Magnoliaceae and compare the genetic difference among generas with inner genera, combining the other research results, the systematic of 20 species in 6 genera was primarily discussed. The purpose of this study was to provide scientific basic data for systematic and other research on Magnoliaceae.

应用RAPD和ISSR标记对木兰科6属20种植物的遗传多态性和亲缘关系进行分析,比较属间、属内的遗传差异,并结合前人的研究结果,对木兰科6属及属下类群的系统位置进行探讨,旨在为木兰科植物的系统分类及其它研究提供实验依据。

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