期

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

So, the nucleocytoplasmic ratio decreased. 10^(-5)MATRA significantly suppressed the proliferation of os732 with only 39.5% cells when compared with untreated cells. Cells in G1 phase increased significantly, and the number of S phase decreased. Expressions of cy-clinD1, cycimD2 and CDK4 mRNA declined, especially cyclinD2 and CDK However, expressions of cyclinD3 and P21WAF1 mRNA changed little.

结果 ATRA作用后，OS732细胞胞浆展开，细胞核变小，核浆比例变小；瘤细胞生长减慢，细胞计数为正常对照组的39.5%； G0/G1期细胞明显增多，S期细胞减少，细胞阻滞于G0/G1期；CyclinD1、CyclinD2、CDK4mRNA表达均下降，尤以CylinD2、CDK4下降最明显，CyclinD3、P21WAF1mRNA未见明显改变。

At water temperature of 17-18 ℃, prelarva stage development of Lucioperca lucioperca lasted for 159 h, which could be divided into 5 stages: hatching stage, xanthic eye stage, melanoid eye stage, one chamber air bladder stage, and exhaustion of yolk stage.

梭鲈的仔鱼发育过程可分为出膜仔鱼、眼黄色素期、眼黑色素期、鳔一室期、卵黄吸尽期5个发育期，在17~18℃水温下历时约159 h。

At a later date the wish to realize the exact number of forty days led to the practice of beginning Lent upon our present Ash Wednesday, but the Church of Milan, even to this day, adheres to the more primitive arrangement, which still betrays itself in the Roman Missal when the priest in the Secret of the Mass on the first Sunday of Lent speaks of "sacrificium quadragesimalis initii", the sacrifice of the opening of Lent.

在稍后的日期，希望实现的确切人数四十天导致实践四旬期开始后，我们目前的圣灰星期三，但教会的米兰，甚至到今天，坚持以最原始的安排，仍然暴露出自己的罗马弥撒时，牧师在地下的秘密的第一个星期日的四旬期提到" sacrificium quadragesimalis initii "，牺牲四旬期开幕。

Wuxia area was located on the eastern of northwest Junggar basin, it was a zone of bruchfalten affected by multiphase tectonic activity, have many faults and its fold morphology were very complicated, underwent three large structural activities, which happened in the late Hercynian movement, Indo-Chinese movement, and the Yanshan movement, by this,strata of Wuxia area can be divided into two primary tectonic sequences and seven secondary tectonic sequences, on plane, Wuxia area can be divided into three secondary structural units from north to south,they are foreland thrust belt, Wuxia fault fold belt and monocline belt, further, from west to east Wuxia fault fold belt can be divided into four fault fold belts.

乌夏断裂带位于准噶尔盆地西北缘冲断带东段，是一个受多期构造叠加影响的断褶带，断裂发育，褶皱形态复杂，自成盆期以来经历了海西、印支、燕山和喜马拉雅等多期构造运动，可划分为2个一级构造层序和7个亚构造层序，可划分出山前冲断带、乌夏断褶带、南部单斜带三个次级构造单元和四个更次一级的构造单元。

In addition, 19 (50.0%) embryos were considered to be chromosomal mosaics due to diploid mosaicism and abnormal mosaicsm.

在≤4细胞期胚胎中，嵌合型胚胎占18.2%，但在5～8细胞期和≥9细胞期胚胎中，嵌合型胚胎的比例分别增至68.4%和50.0%。

A single-factor experiment was designed to research the effect of nutrition level on body weight of multiparous sows during gestation through investigating the effect of gestation nutrition on the reproduction performance of multiparous sows in subsequent 3 parities and based on regression analysis of feed intake as well as lactation weight loss and reproduction performance during gestation.

摘要采用单因子试验设计，通过对妊娠期不同营养水平连续3胎经产母猪生产的研究，建立妊娠期采食量与泌乳损失及繁殖性能等之间关系的回归方程，研究妊娠期不同营养水平对经产母猪体重变化的影响。

objective to discuss perioperative complication and management of patient with high risk copd following esophagectomy.methods 45 patients with high risk copd underwent esophagectomy with epidural block combined with general anesthesia.perioperative treatment included smoking cessation,chest physiotherapy,prevention and control of infection of air way and appropriate bronchodilators of air way,breathing exercises,nutrition support and oxygen therapy.painkiller was instilled after surgery,early exercises,ensuring unobstruction of the air way,mechanical ventilation was applied when needed.results 3 had hypoxaemia in operation.after surgery,all patients had spo2 somewhat declined.6 had lung infection.6 were removed of bronchial secretion by bronchoscope.2 were supported by ventilator by using intubation.2 underwent tracheotomy.1 had disturbances of acid base balance and treated by using hydrochloric acid muriatic acid.all patients recovered rather smoothly and discharged from hospital.conclusion high risk copd is not the absolute contraindication of esophagectomy.active management before and after surgery ensures the safety and recovery of patients.

目的 探讨重度慢性阻塞性肺疾病简称慢阻肺病人食管切除术围手术期常见并发症及其处理。方法 45例重度慢阻肺的病人在全麻联合硬膜外阻滞下进行开胸食管切除手术，围手术期处理包括术前戒烟、胸部理疗、预防和控制呼吸道感染、解痉化痰、呼吸功能锻炼、营养支持和氧疗；术后硬膜外镇痛、早期锻炼、保持呼吸道通畅，部分病人予以呼吸支持。结果术中3例出现低氧血症。术后所有病人pao2均有不同程度的下降，6例出现肺部感染，6例行纤维支气管镜吸痰，2例通过气管插管给予呼吸机支持，2例行气管切开术，1例酸碱平衡紊乱使用盐酸精氨酸治疗。所有病人均痊愈出院。结论重度慢阻肺病人并非开胸食管切除手术的绝对禁忌证，积极的术前准备和严格的术后管理可减少和控制术后急性发作，有助于确保此类病人的围手术期安全和康复。

Methods 75 patients received the examinations of nasality and NAR using Nasometer and Atmos Rhinomanometer before and after ESS, separately.

对资料记录完整的75例成人慢性鼻窦炎Ⅰ型患者（1期38例，2期巧例，3期22例），ESS前后使用鼻声图仪和鼻压计分别对鼻音和鼻阻力进行检测。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。