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Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示:脾脏PCR检测结果表明1、2两实验组均有阳性病例出现,而对照组全部为阴性,说明实验鸡体内已有ALV-J内蒙株感染;接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化:肝脏、脾脏有程度不同的肿大,镜下观察,骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生,随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶,而对照组没有出现明显病变;免疫状态方面:以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强,ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

In DM group, decreasing of density of axoplasm, deposition of fat droplets, swelling and vacuolization of mitochondria and separation or disintegration in lamellar of myelin sheath of the sciatic nerve fiber could be observed, which could be partially improved by EA and KBK.

电镜形态学分析见造模两个月的糖尿病大鼠坐骨神经髓鞘板层有分离现象,线粒体嵴紊乱,有的线粒体嵴完全消失形成空泡线粒体,轴浆内有大量脂肪颗粒沉积;经电针和渴痹康治疗后能减轻这些病理改变。4。无论是电针治疗还是渴痹康治疗均无明显降血糖作用。

Results: In the 23 cases of disease group, 16 cases were unilateral and 7 cases were bilateral. X-ray examination showed local prominence between ischium and pubis in all cases. Lamellar changes of the bone inhomogeneous in density with round translucence were found in 8 cases accompanied with intact cortex. Mild osseous hyperplasia was found in 8 cases without changes of periosteum and soft tissues. CT examination of 8 cases presented with local prominence between iscbium and pubis, translucence, cataclasm, discontiguous osteal cortex with irregular ends, sclerotic medullary cavity of honey surroundings, but no sequestration and changes of periosteum and soft tissues. The above-mentiooed X-ray features appeared in 14 cases among normal group, bilateral in 3 cases.

结果:病变组23例中,16例为单侧,7例为双侧。X线表现为坐骨耻骨结合处局限性骨膨隆,8例有层状改变,病变区内密度不均,有类圆形低密度透光影,骨皮质完整,8例周围骨质轻度增生,未见骨膜反应和软组织改变。8例CT表现为坐骨耻骨结合处局部膨大,其内有低密度透光区,有碎裂,骨皮质连续性中断,断端边缘欠整齐,邻近的髓腔轻度硬化,无明显的死骨和骨膜反应,邻近的软组织正常;正常组中14例出现上述X线改变,其中3例为双侧性。

Results We found GM1 antibody in 36% and cephalin antibody in 43% of MS patients in serum,GM1 antibody in 11% and cephalin antibody 18% in cerebrospinal fluid.The levels of antibody in serum of treated_MS patients were significantly lower than those in active MS patients.The levels of myelin basic protein in serum and cerebrospinal fluid in MS were significantly higher than those in control group.

结果 多发性硬化患者血清GM1抗体阳性率 36%,脑磷脂抗体为43%;脑脊液GM1抗体为11%,脑磷脂抗体为18%,与对照组比较差异均有显著性;血清和脑脊液的髓鞘碱性蛋白增高亦有意义。

Results We found GM1 antibody in 36%and cephalin antibody in 43%of MS patients in serum,GM1 antibody in 11%and cephalin antibody 18%in cerebrospinal fluid.The levels of antibody in serum of treated_MS patients were significantly lower than those in active MS patients.

结果 多发性硬化患者血清GM1抗体阳性率为36%,脑磷脂抗体为43%;脑脊液GM1抗体为11%,脑磷脂抗体为18%,与对照组比较差异均有显著性;血清和脑脊液的髓鞘碱性蛋白增高亦有意义。

Results: electronic microscope showed that in prednisone group heterochromatin increased in some cells, entoblast was not obvious in some cells, fat drops were small and few, mitochondria vacuole were common, and myelin figure was found in smooth endoplamic reticulum. the ultrastructure in yc group was almost normal and megamitochondrion formation was common. a large amount of fat drops were found in cytoplasm in gw group and little improvement was found compared with that in prednisone group.

结果: 电镜结果显示:激素组个别细胞异染色质增多,核仁不明显,脂滴显著变小变少,线粒体空泡化常见,有滑面内质网髓鞘样结构形成;养阴组电镜结构基本正常,其特征是细胞内有较多的巨大线粒体形成;桂附组较激素组改善不明显,其特征是胞质内可见大量脂滴。

The same results were observed in the brain of the unilateral pyramidotomy group above the level of the transection site. Below the level of the transection site, the BDA positive fibers were seen in the right pyramid of medulla oblongata and the left dorsal funiculus of spinal cord, but the BDA positive fibers in the left pyramid of medulla oblongata and the right dorsal funiculus of spinal cord were not seen.

锥体束横断损伤大鼠在损伤部位以上可见双侧对称的BDA阳性纤维束,损伤平面以下在右侧延髓锥体和左侧脊髓后索中见有BDA阳性纤维束,损伤侧锥体和右侧脊髓后索中未见有BDA阳性纤维束。

Results there were unconsciousness, respiratory rhythm disturbance and hyporeflexia of pupil light reflex immediately after injury, and reactiveness decrease and activity retardation still existed even after resuming consciousness. at anatomical scene, subarachnoid hemorrhage or cerebroventricular haemorrhage were widespread. at an early stage, there were swelling, collapse, and axonal retraction ball formation at cortico-medulla junction, callosum, brainstem, and cerebellar white matter under microscope. but at the later stage, gitter cell proliferation and nest-like aggregation were major pathophysiological changes at focal brain tissue.

结果 伤后大鼠均即刻出现原发昏迷,其中2只于损伤后20 min内死亡,余持续时间1-30 min不等;伤后大鼠呼吸节律紊乱,瞳孔对光反射减弱或消失,醒后均有程度不等的反应性下降,肢体活动迟缓;肉眼可见广泛蛛网膜下腔出血或脑室出血;光镜下可见皮髓交界区、胼胝体区、脑干、小脑白质等部位的神经轴索有不同程度的肿胀、断裂、轴索球形成,后期有小胶质细胞增生,局部呈巢样聚集。

Histology and structrure of uteruses were not significantly different either, andendometrium, myometrium, perimetrium included.

皮质内含有黄体和不同发育阶段的卵泡等,髓质有疏松结缔组织构成,含有血管、淋巴管和神经等。

Results: group A( diskectomy, 160 cases) were followed up and excellent: short-term 90%,in the near future 88%, long-term 84%;36 cases were accepted second operation during 0.5 year to 5 year and 28 cases had nerve root canal stenosis.

结果:A组随访总有效率分别为:短期90%,近期88%,远期84%;有36例(22.5%)患者在0.5-5年间接受了二次椎间盘手术,其中28例原因为神经根管狭窄。B组(髓核摘除并神经根管探查、扩大术组)168例病例术中发现88例(52.4%)有不同程度神经根管狭窄,且均为年龄40岁以上、平均病程5.8年的患者;随访总有效率分别为:短期98%,近期95%,远期92%。

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