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ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果:hMSCs诱导前为纺锤形,诱导后第2天部分细胞即开始发生形变,呈球形或短棒状,1周后胞浆中颗粒增多,约20~30%细胞边缘呈毛刷样变化;hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义,CD90未诱导时表达呈弱阳性,诱导后明显增高(P.05);ANP和CX43在诱导前无表达,诱导后第2周开始表达且表达随时间逐渐增强,但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加,低浓度诱导组低于标准浓度诱导组,组间差异有统计学意义(P.05),G_0/G_1期细胞比例诱导后较对照组显著减少(P.05);β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强,在第6周时均达到高峰,第8周时表达开始衰减,Bcl-2、Bax基因表达呈时间依赖性变化,hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

Results IL-6R immunoreactive cells were observed in the anterior of optical area,the ventromedial nucleus,the periventricular nucleus and arcurate nucleus of the hypothalamus.The strongly stained cells are concentrated in the granule cell layer of the dentdate gyrus and the pyramidal cells of the CA1-CA2 fields of the hippocampus.IL-6R positive cells are also detected in the cortex, the ventromedial nucleus of the thalamus,bed nu stria,olfaltory tubercle.

结果 IL-6R 免疫反应性细胞主要分布在下丘脑的视前区、腹内侧核、室旁核和弓状核;在海马, IL-6R 阳性细胞呈强阳性,密集分布于齿状回颗粒细胞层和 CA1~ CA2锥体细胞层;在大脑皮层、嗅结节、丘脑腹内侧核、终纹床核等处也有 IL-6R 阳性细胞。

During the acclimating process, CODcr, DO, nitrite, UDMH should be measured.

培养的活性污泥颗粒大、沉积快、出水良好、生物相镜检有轮虫。

Leading products are: ZSG series efficient heavy screen, GQMS high rate of powerful special screening of coal, such as thick oval Shaker, YA type YK-round vibration sieve, SZR series of thermal mineral shaker, MZS series of pulverized coal shaker, ZWS vibration Network Screen, K-type reciprocating to the coal machine, ZFB series of anti-blocking, DKS granular urea screening machines, ZG series motor vibrating feeder, GZD electromagnetic vibrating feeder, the feeder disk, LS, GX-screw conveyor, PL, D-type bucket elevator, DJ, JB-steep, vertical belt with corrugated wall machines, mobile ZP-belt conveyor, Gechongfeibiao structural design and fabrication.

主导产品有:ZSG系列高效重型筛、GQMS高幅强力煤炭专用筛、椭圆等厚振动筛、YA型、YK型圆振筛、SZR系列热矿振动筛、MZS系列煤粉振动筛、ZWS振网筛、K型往复给煤机、ZFB系列防闭塞、DKS大颗粒尿素筛分机、ZG系列电机振动给料机、GZD电磁振动给料机、圆盘给料机、LS、GX型螺旋输送机、PL、D型斗式提升机、DJ、JB型大倾角、垂直波状挡边皮带机、ZP型移动式胶带输送机、各种非标结构件的设计及制造。业务覆盖全国各地,远销国外。

Leading products are: ZSG series efficient heavy screen, GQMS high rate of powerful special screening of coal, such as thick oval Shaker, YA type YK-round vibration sieve, SZR series of thermal mineral shaker, MZS series of pulverized coal shaker, ZWS vibration Network Screen, K-type reciprocating to the coal machine, ZFB series of anti-blocking, DKS granular urea screening machines, ZG series motor vibrating feeder, GZD electromagnetic vibrating feeder, the feeder disk, LS, GX-screw conveyor, PL, D-type bucket elevator, DJ, JB-steep, vertical belt with corrugated wall machines, mobile ZP-belt conveyor, Gechongfeibiao structural design and fabrication.

主导产品有:ZSG系列高效重型筛、GQMS高幅强力煤炭专用筛、椭圆等厚振动筛、YA型、YK型圆振筛、SZR系列热矿振动筛、MZS系列煤粉振动筛、ZWS振网筛、K型往复给煤机、ZFB系列防闭塞、DKS大颗粒尿素筛分机、ZG系列电机振动给料机、GZD电磁振动给料机、圆盘给料机、LS、GX型螺旋输送机、PL、D型斗式提升机、DJ、JB型大倾角、垂直波状挡边皮带机、ZP型移动式胶带输送机、各种非标结构件的设计及制造。

Many anaerobes were killed in sludge from UASB during the 6 months of acclimation, which resulted in decreased dechlorination rate and SMA.

对UASB反应器颗粒污泥的间歇驯化过程中厌氧微生物大量死亡,脱氯速率和比产甲烷活性比驯化前有明显下降。

Shanghai SAFRAN Packaging Machinery Ltd., founded in 2005 in Shanghai, the Secretary's main business is the manufacture and packaging machinery sales, the main products have pillow charter, angle packaging machine, large vertical packaging machine, granule packing machine, packaging paste machine, bag packaging machines, balers and other dozens of products.

上海赛峰包装机械有限公司于2005年成立于申城,本司主要业务是包装机械的制造与销售,主要产品有枕包机,折角包装机,大型立式包装机,颗粒包装机,膏体包装机,袋泡茶包装机,打包机等数十种产品。

Results:The low, middle and high doses of both the traditional slice and dispensing granule Si-Wu decoctions apparently increased the number of circulating blood cells.

结果:四物汤配方颗粒汤剂与传统饮片汤剂不同剂量组均有不同程度升高外周血细胞的作用,两者无显著性统计学差异。

One out of three leyers of all fields in the hippocampus cortex had marked distinction (0. 01<P<0.05), stratum multifrom cell>stratum molecule>stratum pyramidal cell of stratum granule.

比较各区域海马皮质的三层机构毛细血管比容积,也有明显差异(0.01<P<0.05),多形细胞层>分子层>锥体细胞层或颗粒层。

A grinding mill has a rotating container (40) into which particulate material is fed.

一种磨碎机具有一颗粒物料供入其中的容器(40)。

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