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It is important that rhGM-LIF can inhibit the growth of human liver cancer cell.

值得重视的是,融合蛋白对人肝癌细胞的生长也有明显的抑制作用。

A rightward shift of the signal was observed signifying a reduction of the uptake of the fusion protein GST-GFP-Tat at 4℃compared to its cellular uptake at 37℃.

其三,温度对跨膜递送作用影响较大,37℃条件下有高跨膜效率的融合蛋白 4 ℃时跨膜递送作用几乎完全被抑制。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的:从红花五味子果实中寻找活性代谢产物,研究其抗HIV—1活性和作用机制方法:通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验,检测分离化合物的抗HIV—1活性并探讨其作用机制一结果:红花五味子甲素能够抑制病毒诱导的合胞体形成,保护病毒感染细胞,抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^+细胞间的融合,逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制,也不抑制蛋白酶的活性、结论:首次从天然产物中分离得到红花五味子甲素,它对HIV—1病毒复制早期具有抑制作用

3Goal setting increased the occurrence of flow experience,especially on thedimensions of Action-awareness merging,Sense of control,Loss of self-consciousness,Autotelic experience,and the total for the dispositional flow,while onthe dimensions of Challenge-skills balance,Sense of control,Loss of self-consciousness,Autotelic experience,and the total for the state flow.

3目标设置能促进体育运动中流畅状态的发生,其对特质流畅中&行动-意识融合&、&控制感&、&自我意识的丧失&和&享受的体验&四个维度及总分有显著促进作用;对状态流畅中&技能-挑战平衡&、&控制感&、&自我意识的丧失&和&享受的体验&四个维度及总分有显著促进作用。

Specifically, itcontains 8 chapters.In chapter 1, the formation, structures, properties and the futureprospect of liposome were thoroughly reviewed;In chapter 2, the stibility and permeability of phopholipid -eleostericacid liposome were studied together with the effect of polymerizationof eleostearic acid. This membrane system was very sensitive to 〓,the effect of 〓 was clarified to increase the aggregation/fusion ofliposomes and made the permeability of mixed liposomes much higher;In chapter 3, two polymerizable conjugated diyne bolaamphiphiles were synthesized. They could form very stable mixed liposome, andthe diyne could be polymerized by UV light in bilayer liposomes, as aresult, the stability of mixed liposome against solvent or surfactantafter polymerization were enhanced. In chapter 4, two kinds of amphiphilic amino acids were synthesized andstable liposomes were formed therefrom After the condensationpolymerization of amino acid in bilayer liposomes, stable polypeptide liposomes were obtained, which had lower phase transition temperatureand higher permeability.In chapter 5, four kinds of glycolipids were synthesized and theiraggregation behavior in water was comparied. When incorporated intophospholipid bilayer membranes, they could increase the phase transitiontemperatures and inhibit the aggregation and fusion of mixedliposomesat lower temperature.In chapter 6 and 7, three kinds of steroidal bolaamphiphiles withdifferent chain lengths were synthesized. Incorporation of steroidalmoiety to the center of lipid bilayer membrane obviously increased themobility of lipid membrane and shifted Tc to lower temperature side incomparasion with cholesterol. The bolaamphiphile which was shorter thanthe hosted lipid bilayer membrane thickness influenced the lipid packingmore obviously.

全文共分8章:第一章对脂质体的形成、结构、性质及展望进行了较为详细的文献综述;第二章研究了磷脂-桐酸脂质体的稳定性,通透能力及桐酸的聚合对这些性质的影响;磷脂-桐酸混合脂质体为一类对〓灵敏的脂质体,〓的作用首先是使脂质体集聚然后使脂质体融合,并加速内包荧光物的释放;第三章通过合成两种可聚合共轭双炔双极性双亲分子DDCA,DDOL,研究了共双炔分子在双分子层脂质体膜上的聚合及对脂质体性质的影响,聚合可以提高脂质体相对于溶剂及表面活性剂的稳定性;第四章合成了两类氨基酸为极性基团的双亲分子,它们均可以在超声下形成稳定的脂质体结构;氨基酸基团可以在脂质体上进行缩聚反应,若聚合后脂质体表面仍有足够的亲水能力,则可得到稳定的多肽型脂质体;聚合后脂质体的相变温度降低,通透能力增加;第五章合成了四种亲水基团为单糖基的双亲分子GL-l,GL-2,GL-3, GL-4,研究了它们在水中的分散情况、集合体形态与分子结构的关系;在DMPC双分子层膜中加入糖脂分子可以使脂质体的相变温度提高,阻止脂质体在低温放置时的集聚与融合;第六章-第七章合成了三种不同碳链长度的双极性含胆甾环双亲分子 CL-1,CL-2,CL-3;它们可以象胆固醇一样与磷脂混合形成稳定脂质体,胆甾环基团位于脂质体双分子层膜的中间;与胆固醇的作用相反,它们可以增加磷脂双分子层膜的流动性,降低混合脂质体的相变温度;三种分子的作用与其碳链长度和磷脂双分子层膜的厚度有关,比膜厚度短的分子影响最大。

Myoblastic cells from patients with PM had a weaker ability of developing into the myotubules, thus they were unable to perform effective regeneration, which resulted in a progressive necrosis. The exogenous bFGF could promote the division and proliferation of the muscle cells in vitro . These results shield a light on bFGF's potential role in preventing and treating PM.

PM患者受损腓肠肌在体外有再生能力,但融合成肌管能力极弱,存在再生障碍,不能对变性、坏死的肌纤维形成有效再生,故呈进行性肌纤维耗竭;外源性bFGF可促进PM患者体外培养骨骼肌细胞增殖分裂,并可促进肌管成熟,对进展性肌病应具有防治作用。

But science neither simply opposes against metaphysics nor simply merges with it, their relationship is complex: they are interdependent, interpenetrated, mutually conditional, mutually conductive, and work in coordination, at the same time, they are mutually exclusive, and hold each other back. Besides these harmful influence and positive contribution, there're more other interactions in varied dimensions, which are determined by specific scientific theory and specific metaphysical one.

本文认为,形而上学是科学之所以可能的一个主体条件,同时又是科学发展的一个限制性条件,但是,科学和形而上学之间既不是简单的融合也不是简单的对立,它们的关系具有复杂性:科学和形而上学既相互渗透又互相反对,既相互制约又相互诱导,其作用方式和作用效果又是多重的、有差别的、复杂的,这既取决于具体的科学理论又取决于具体的形而上学学说。

But scienceneither simply opposes against metaphysics nor simply merges with it, their relationship is complex: they are interdependent, interpenetrated, mutually conditional, mutually conductive, and work in coordination, at the same time, they are mutually exclusive, and hold each other back. Besides these harmful influence and positive contribution, therere more other interactions in varied dimensions, which are determined by specific scientific theory and specific metaphysical one.

本文认为,形而上学是科学之所以可能的一个主体条件,同时又是科学发展的一个限制性条件,但是,科学和形而上学之间既不是简单的融合也不是简单的对立,它们的关系具有复杂性:科学和形而上学既相互渗透又互相反对,既相互制约又相互诱导,其作用方式和作用效果又是多重的、有差别的、复杂的,这既取决于具体的科学理论又取决于具体的形而上学学说。

Jilin City in stone wine accessories limited liability company is engaged in research and development, production and operation of the wine accessories business, the company's production base is located in Jilin City of Jilin Province of China scenic Lake Song-Hua, followed by the Changbai Mountains Forest wine accessories with production of precious trees Changbai Mountain - oak, with good commercial credit, high quality customer service and professional marketing and technical services, in-market companies in China established an extensive network of customers, and has achieved rapid development, the Company's production Oak accessories, wine can improve the color and enhance the stability of color and improve the structure of wine, liquor enhance the level of flu, make the wine taste more gentle, mellow flavor after long recollection of fullness, but also so that the aroma of wine integration Coordination of grape wine in the minor Green flavor of Health, such as sulfur smell a bad odor masking effect.

吉林市显石酿酒辅料有限责任公司是从事研发、生产、经营葡萄酒辅料的企业,公司的生产基地位于中国吉林省吉林市风景秀丽的松花湖畔,绵绵的长白山林区拥有生产葡萄酒辅料的珍贵树种---长白山橡木,凭借良好的商业信用,优质的客户服务以及专业的市场销售和技术服务,显石公司在中国市场建立了广泛的客户网络,并取得了快速的发展,本公司生产的橡木辅料,可以提高葡萄酒的色泽,增强色泽的稳定性,改善葡萄酒的结构,增强酒的层次感,能使葡萄酒的味觉更加柔和,后味醇厚丰满回味悠长,还能使葡萄酒的香气融合协调,对葡萄原酒中轻微的生青味、硫臭味等不良气味有掩蔽作用。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后,先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时,此时卵母细胞已进入第一次减数分裂的前中期,并且在DIC倒置显微镜下可以看到浓缩的染色体,用Polscope可以发现明显的纺锤体,借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换,经过三次直流电脉冲作用后,分别有91.6%的胞质—MI核质体对融合,经过进一步的培养后,超过80%的重组卵母细胞排出第一极体,体外受精后分别有79%和77%的重组卵形成双原核,受精后的胚胎在KSOM胚胎培养液中体外培养4天后,超过60%的胚胎发育至囊胚。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。