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Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用,并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1),另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死,取出损伤节段脊髓用来组织病理学检查,同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过,到损伤后28天,我们观察发现,损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18,group 1∶9.89±1.29;p.05),光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

As with rhIL-1β, rhTNFα(100ng/ ml) can also provoked ICAM-1 surface expression by HMC. We also found VCAM-1 mRNA expression by HMC in normal culture condition and exposure of mesangial cells to rhTNFαis associated with increased VCAM-1 mRNA lavels rapidly (4 hours).These exploration indicate (1) glomerular and tubular ICAM-1 expression level correlate with inflammatory degree of glomerulus and TNF-αexpression level (2) VCAM-1 may have certain role in patients of lupus nephritis and crescentic nephritis (3) Expression of ICAM-1 and VCAM-1 may be regulated in vivo by cytokines (TNFα and IL-1β).

与轻度系膜增生性肾炎病人血清中的VCAM-1水平比较狼疮肾炎病人血清中VCAM-1的水平显著增加;(3)rhIL-1β(25ng/ml)不但能增加ICAM-1 mRNA的表达,还增加ICAM-1蛋白的表达;rhTNFα(100ng/ml)对ICAM-1蛋白表达和VCAM-1 mRNA基因表达均有明显的上调作用,结果提示:肾小球和肾小管ICAM-1表达水平与肾小球炎症病变程度有关;VCAM-1在狼疮肾炎和新月体肾炎的发生发展中可能有重要作用;体内ICAM-1和VCAM-1的表达可能受TNFα和IL-1β的调节。

Then we elementarily find that the assimilation of the real-time data in high layer and surface i...

初步的结果显示,同化高空和地面实况资料后对模式的初始场有明显的改进、对暴雨过程的降水落区和强度有不同程度的正反馈,更接近实况的降水。

Using assay of 21 Wistar rats from diarrhea intestine separation, pure culture of bacteria in the intestine containing 1% were small thorn Hericium erinaceus polysaccharide fermentation broth and 1% fermentative polysaccharide liquid medium 37 ° C for 18 h after the OD value, Statistical analysis showed Hericium and see 21 species of Ganoderma intestinal bacteria strains of the five species of a clear inhibitory effect other strains of the role is not obvious, Ganoderma and Hericium compared Hericium no significant role in the strain Ganoderma and there are obvious inhibition, Hericium and prominent role in the strain.

采用比浊法测定了21种从腹泻Wistar大鼠肠道内分离、纯培养的肠道细菌在分别含有1%小刺猴头菌发酵液多糖和1%灵芝发酵液多糖的培养基中37℃培养18h后的OD值,经统计分析结果表明见猴头和灵芝对21种肠道菌中的5种菌株有明显的抑菌作用,而对其他菌株的作用并不明显,灵芝和猴头相比较,猴头没有显著作用的菌株,而灵芝却有显的抑制作用,而猴头具有显著作用的菌株。

Chemical analyses of ores sampled from different elevation at the representative Zoujiashan uranium deposit indicate that Fe〓O〓, FeO, MgO and P〓O〓 increase evidently with depth which is related to alteration of haematite, chlorite and apatite, Na〓O and Na〓O+ K〓O also increase with depth, reaching a maximum of Na〓O at 911 meters below the surface, which suggests alkaline alteration increases downwards. At depth of 90 meters below sea level, where the boundary of the two cycles of the volcanic rocks is located, CaO reaches the maximum, representing maximum of alteration of fluorite.

对典型矿床不同富集标高矿体的化学全分析结果表明:随深度增加,Fe〓O〓、FeO、MgO及P〓O〓的含量有明显增高的趋势,这与赤铁矿化、绿泥石化及磷灰石化有关;-90m标高的CaO含量最高,达17.67%,反映这一部位的萤石化最发育;Na〓O及Na〓O+K〓O的含量由浅入深也存在明显的增高趋势,且Na〓O的含量在911m深处为最高,这充分显示碱交代作用从上到下有增强的趋势,而钠长石化在911m处最强烈。

There are many choices we have to make through our life, different decision brings different result, perhaps it means success or failure, Choice is a headachy thing, a lot of people hate to choose, they want to gain but fear to lose, there is an old saying: Look before you leap, yes, that's right, sometimes choice isn't a simple action, it bears more responsibility, more expectation, more pursuit .

在我们的生活中有很多的选择,不同的选择会导致不同的结果,就像赌注,有成功和失败。选择往往是一件令人头痛的事,很多人都讨厌选择。他们都想得到但是却害怕失去,古人云:三思而后行,是啊,说得对极了。有时选择不仅仅是一个简单的行为,而是要在承受着更多的责任,期待和追求。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料,通过9~11 日龄非免疫鸡胚进行了病毒的实验室分离和传代,并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定,结果表明,4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞,标准阳性血清可特异性的抑制其凝集性,回归试验可复制出与自然发病相同的病例,病毒传代物有明显的致鸡胚矮小化作用,透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子,将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

The expression of VEGF was observed by using image analysis and the secretion of VEGF by macrophagus was tested by using immunohistochemistry method.

结果:在口腔鳞癌中,VEGF的表达和微血管计数、巨噬细胞计数呈现正相关(P.05);VEGF的表达、巨噬细胞的浸润和肿瘤的淋巴结转移有关(P.05),口腔癌浸润的巨噬细胞中有29%~10%的细胞内有VEGF的表达。

Items of pH, soil solution K, exchangeable k, nonexchangeable K and potassium fixation index were all determined after incubation. The results indicated similar extent and trends of transformation among three K forms, that is, decreasing content of soil solution K and exchangeable K but increasing nonexchangeable K level were observed in both CaCO3 and SrCO3 treatments of two soils. As to CaCl2.2H2O addition, pH increase was not found and the change of three K forms was not significant when application rate raised.

结果显示CaCO3的添加,除能提升土壤pH值外,亦能使土壤中三种型态钾(溶液性钾,交换性钾,非交换性钾)有显著的改变,且改变程度及趋势均甚为相似,而CaCl2.2H2O的添加不会造成土壤pH的改变,且三种型态钾并不随CaCl2.2H2O用量的增加而有显著改变的现象。

The results showed that the main pathogenic bacteria ledlocal cows to endometritis were streptococcus,the next,Escherichia coli,staphylococcus and pseudomonas aeruginosa.NFPL could quickly reduce thenumber of the bacteria in uterus,and strongerly inhibit these bacteria in vitro.NFPL could excit the uterus,which occurred in enlarging contractionamplitude.NFPL could lower abnormal high content of progesterone and raiseabnormal low oestradiol.

结果表明,引起本地奶牛子宫内膜炎的主要致病菌是链球菌,其次是大肠埃希氏杆菌,葡萄球菌,绿脓杆菌;新促孕液能能迅速减少病牛子宫内细菌的数量,在体外对这些细菌也有较强的抑制作用;新促孕液对兔子宫有明显的兴奋性作用,主要表现在收缩波幅的加大;新促孕液能降低病牛血清异常升高的孕酮含量,升高异常降低的雌二醇含量。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。