有细胞核的

Results By using optical microscope,the neurocytes of hippocampus in experimental group were observed to denaturalize,tumefy,destroy and disappear.And under electron microscope,the karyon was irregular,nucleoles deviated,chondriosome tumefied,crista collapsed,membrane was damaged,substance cavitated.Such changes were more evident with the increased paroxysm of epilepsy,the parameters between control group and operation group had no difference（P＞0.01）.But make a monofactorial variance analysis among cells' number,area,circumference and integral optical density in different groups,it showed that there were significant differences between optional two groups（P.01;at the same time,the more serious injury in hippocampus,the more powerful GFAP immunoreaction was.

结果： 实验组海马区光镜下可见神经细胞变性、肿胀、坏死及神经细胞脱失改变，电镜可见细胞核形态不规则、有切迹、核仁偏位、线粒体肿大、嵴断裂、膜破损和基质空化等改变，随癫痫发作次数增加上述改变越明显，对照组与手术对照组比较，各参数差异无显著性（P＞0.05），将不同组别大鼠细胞数、面积、周长、积分光密度分别作单因素方差分析，不同组别间均差异有显著性（P.01），同时观察到海马区GFAP免疫反应阳性的胶质细胞随海马损伤的加重而GFAP免疫反应增强。

Endomitosis The duplication of chromosomes without division of the nucleus, causing polyploidy and leading to an increase in nuclear and cytoplasmic volume.

核内有丝分裂：在细胞核不分裂的情况下进行染色体复制，产生多倍体并使细胞体积增大的一种有丝分裂形式。

The morphological and anatomical characteristics of"Zha′ai 76"Comparing with the plants of normal M.baccata and ordinary hybrid,"Zha′ai76"and other plants with Dw major gene showed that:the length of springgrowth or main shoot is shorter by 59.6-66.2%;the ratio of length:diame-ter of new shoot smaller by 33.2-58.4%;the length of internee shorter by14.0-42.7%:the node number of spring growth lesser by 40.7%;the cauli-cle height under the cotyledons of hybrid seedlings shorter by 65.0%;the in-dex of leaf stalk(stipe length:stipe thiek ness)on the spring growth smaller by34.5-50.2%;the length of leaf stalk shorter by 25.2-45.7%;the boundarybelt between spring and autumn growth has knob-like nodes;the margin of leafappears wave-like;the sepals with spinous proeessuses are massive and small;the eonstriction at calyx is not obvious;the trace of abscisic sepals is big;the ca-lyx end of fruit is plan or convex;the shape of shoot tip(of"Zha′ai 76")iswider and shorter;and in 1.0mm part from the top of shoot tip,there are manyspecialized cells which are larger in volarae,but with lesser inner material andwith smaller or no nucleus.

"扎矮76"的形态解剖特征与普通山定子和杂交后代中普通型植株对比，"扎矮76"和其它携带Dw主基因植株的：春梢短59.6-66.2％，梢长与梢粗比值小33.2-58.4％，节间短14.0-42.7％，春梢节数少40.7％，杂种苗下胚轴短65.0％，春梢叶柄形指数小34.5-50.2％，叶柄短25.2-45.7％，春秋梢交界处有瘤状突起，叶缘波状，萼片肥厚有刺状突起，萼部缢痕不明显，脱萼后萼痕较大、萼洼平或高于果面；新梢生长点宽而短，在距顶端1.0gmm处有较多体积较大、内容物较少、细胞核小或无的待化细胞。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩，管壁增厚呈空虚网状，生精细胞层次减少，间质细胞增生，但仍见有精子生成；高剂量组可见到精子呈异型性改变，细胞核浓缩深染，且随着摄入时间延长改变愈趋明显；F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩，间质增生明显，20个月时肾小球萎缩纤维化；F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生，淋巴母细胞增生活跃，20个月时脾小体减少，生发中心稀疏；F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润，晚期骨髓有核细胞减少，脂肪细胞增加。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

In male, FSH and LH immunopositive substance was distributed in the right and left part of procerebrum at flying stage.

本研究首次证实白蚁婚飞行为与其前脑部神经活动有密切关系；原癌基因c一fos在调节与性行为有关的前脑神经细胞核内基因表达方面有重要作用。

At last instar nymph and after flying stage, c-fos immunoreactivity of procerebrum was weak, but the female and male termites displayed significantly increased number of c-fos labeled cells in the protocerebrum at flying stage.

本研究首次证实白蚁婚飞行为与其前脑部神经活动有密切关系；原癌基因c-fos在调节与性行为有关的前脑神经细胞核内基因表达方面有重要作用。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下，卵母细胞外的卵丘细胞紧紧包裹卵母细胞，且与卵母细胞之间有大量细长的微绒毛相联系；卵丘细胞核呈卵圆形，其长径为4250±0.042μm，短径为2.750±0.071μm；透明带均匀，与卵母细胞结合紧密；在皮质区集中分布大量的多峰型线粗体、脂滴和空泡，线拉体长径为0.600±0.106μm，短径为0.490±0.117μm；细胞核中颗粒状的核质分布非常均匀。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。