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Some investigations suggested that cyclins and CDK played an important role in the regulation of endomitosis of megakaryocyte. However, the mechanism of cyclins and CDK in the regulation of endomitosis of fetal megakaryocytes during ontogenesis and the relationship between cyclins/CDK and retardation of endomitosis of fetal mgakaryocyte is totally unknown.

一些研究发现周期蛋白和CDK在巨核细胞核内有丝分裂的调控上起重要作用,然而,周期蛋白和 CDK在胎儿巨核细胞核内有丝分裂调控上所起的作用,及其与胎儿巨核细胞核内复制延迟的关系目前全然不知。

Nuclei are quite scattered and have only small, granular clumps of blue heterochromatin.

高倍镜显示平滑肌的胞浆嗜酸性,细胞核散在,核内有细小的颗粒状的蓝色的异染色质。

(1) In our research,frequent expression of BSAP in H/RS cell of classical Hodgkins disease provides further evidence for its B-cell origin,and the expression of BSAP on H/RS cells also can be in favor of the identification of H/RS cells and can distinguish HL from ALCL.(2) Expression of BSAP is located in nucleoli of cells,while that of CD20 in membrane.

结果 (1)33例cHL中,30/33(90.91%)H/RS细胞和33/33(100%)背景淋巴细胞BSAP表达阳性,而H/RS细胞10/33(30.30%)CD20阳性,1/33(3.03%)CD45RO阳性,31/33(93.94%)CD30阳性,25/33(75.75%)CD15阳性;(2)33例HL石蜡刮片组织中有16例(48.48%)出现IgH基因重排阳性,显微切割的19管H/RS细胞有14管(73.68%)扩增阳性,而12管背景淋巴细胞有2管(16.67%)扩增阳性;(3)测序分析周新华第一军医大学病理学教研室获得了一段免疫球蛋白重链可变区的重排片断;(4)原位PCR结果发现杂交的阳性颗粒不仅出现在探针来源的同一病例也出现在不同病例的HillS细胞核上,同时在部分周围背景淋巴细胞核上也见到阳性颗粒。

The nuclear image messages were digitalized and stored in the computer to form a database available for further study in this field. Results (1) In the EL, the majority were the big global nuclei of the mature ependymal cells. A few bigger nuclei were located deep to the ependymal cells, and some small irregular-shape, nuclei were scattered and wedged between ependymal cells.

结果 ①在EL,主要是体积较大且呈球形的成熟室管膜细胞核;另有体积更大的球形细胞核贴近室管膜细胞深方;此外,还有体积较小而形状不规则的细胞核散在镶嵌于室管膜细胞之间。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分,减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常,使多种脏器组织的细胞浆内核糖体丰富,线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

In the past twelve years there have been hundreds of studies showing these nonthermal effects--- such as DNA damage and nonrepair, opening of the blood-brain barrier (allowing toxins and pathogens to reach the brain), lowered immunity, decreased melatonin levels, effects on stress proteins, formation of micronuclei (aberrations in cell nuclei which are often markers for cancer), changes in calcium metabolism affecting communication between cells, changes in brainwave patterns as seen on EEG's, plus effects observed on many different systems of the body.

在过去十二个月里,已有数以百计的研究表明这些非热效应---如DNA损伤和nonrepair ,开放血脑屏障(允许毒素和病原体到达大脑),降低免疫力,降低褪黑激素水平,对应激蛋白,形成的微(畸变细胞核往往是癌症的标志),改变钙代谢的影响细胞之间的沟通,改变脑电波模式脑电图上看到的,加上效果观察许多不同的系统的机构。

Several appear here as large cells with two or more nuclei that have prominent nucleoli.

图示可见有几个细胞内有2个或2个以上的细胞核形成了巨大的细胞核。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明,AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化;TUNEL检测呈阳性,说明有染色体DNA出现断裂;Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露;电镜观察的结果显示,转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

Every cell with a nucleus has chromosomes that carry genes.

每个细胞都有一个细胞核,细胞核中的染色体携带有遗产基因。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。