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Wilms tumors with unfaorable histology contain anaplasia – defined as the presence of hyperchromatic, enlarged nuclei (at least 3 times as large as blastemal nuclei), and multipolar mitotic figures.

如有细胞间变则提示Wilms'瘤预后不良,所谓细胞间变,表现为染色质粗糙,胞核增大(至少是后肾胚芽细胞核的3倍大小)以及多极性有丝分裂像。

Among these features are: absence of the nuclear rotation and development of the centriolar complex and the initial region of the axoneme in a position directly perpendicular to the basal pole of the nucleus and presence of numerous thick anchoring fibrils connecting the proximal centriole and the proximal part of the distal centriole to the nucleus.

其特点主要是:生精过程中不发生细胞核的旋转,中心粒复合体和轴丝起始段直接发生在核的基底面垂直线上,有无数的粗的固定纤维将近端中心粒和远端中心粒的近侧部连接到细胞核上。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔,然后一边缓慢地将注射针回拔至卵周隙中,一边逐渐增加持卵针中的负压,直至极体与目标核质被完整吸入持卵针中而完成去核,最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

Observ ed with transmission electron microscope and scanning electron microscope, t he apoptotic nuclei showed the typical morphological feathers of apoptosis: cond ensation and margination of chromatin, bubbling of nuclear membrane and apoptoti c bodies.

在透射电子显微镜和扫描电子显微镜下都观察到凋亡的细胞核呈现典型的凋亡形态学特征,例如染色质的凝集及趋边化,细胞核表面形成空泡化结构并有大量的凋亡小体结构出现。

Methods: Immunohistochemistry and image analysis techniques are applied to examine the phosphorylated extracellular signal-regulated kinase in epidermis of non-expanded skin and expanded skin. Results:Phosphorylated ERK expressed in basal cell layer of epiderm including non-expanded skin and expanded skin are expressed. But in expanded skin they are expressed significantly and densely compared to non-expanded skin. There are significant difference in relative to location of expanded skin and regions of expanded skin.

结果: 1扩张皮肤和正常皮肤表皮基底层都有磷酸化ERK 的表达和分布,但扩张皮肤中的磷酸化ERK的分布和表达较明显,染色较深且密,部分阳性细胞呈多层排列,有散在的增殖团区;2扩张皮肤的顶部和侧部差别不十分明显,而侧部和顶部与基底部有一定的差别,基底部染色较深,多为细胞核着色;3 经图像分析不同部位的正常皮肤和扩张皮肤以及扩张皮肤的不同部分的相对灰度值和阳性细胞密度,进一步证实了上述观察结果。

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明,枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成,是位于背部的一个囊状结构,它由两部分构成,前部的方形囊状体和后部的三角形囊状体,二者之间有一突起的脊;雌蛾静止时,腺体随第八和第九腹节一起嵌缩于第七腹节内,求偶时,腹部末端外伸,腺体细胞表皮外露,释放性信息素;腺体细胞呈方形,其表面覆盖几丁质表皮,表皮顶端无孔;细胞核很大,呈椭圆形,由双层核膜包被,位于细胞中下部,内有丰富的染色质;细胞质内有丰富的内质网,其内还有大量的大小不等的脂肪滴,以及线粒体和溶酶体等细胞器。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果:倒置显微镜和透射电镜观察显示,经SAHA处理的细胞增殖速度显著减慢,细胞体积增大,细胞核较小,形状较为规则,核仁数量减少、体积变小,核内常染色质增多而异染色质减少,核质比例减小,细胞质内线粒体数量增多、线粒体嵴发达,高尔基体较为典型,粗糙型内质网增多,呈现出与正常上皮细胞相似的形态变化;MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用,并有明显的剂量依赖和时间依赖关系;免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达;流式细胞仪检测结果显示,SMMC-7721细胞经SAHA处理后,G0/G1期细胞明显增加,S期细胞则明显减少,细胞被阻滞于G0/G1期;RT-PCR检测结果表明,SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加,24h后更为明显。

The nuclei of the Paget's cells are atypical and, though not seen here, often have prominent nucleoli.

Paget氏细胞的细胞核有异型性而且常有显而易见的核仁。

At the age of 19 weeks, we have observed ciliated-mucous cells, some cells presented with mucous granules in the supranuclear cytoplasm and a ciliating border with short growing shafts, while the other cells presented with large mucous granules as well as compound cilia.

小粘液颗粒细胞呈柱状,胞浆顶部含有少量球形的粘液颗粒,细胞表面有短小的微绒毛,细胞核大呈椭圆形,胞浆电子密度高,胞浆下部有粗面内质网和线粒体,核上部有高尔基体。

Diseased cobias were collected from some net-cage farms at Hsiqu Liouchiou Island, next to Dunggang, Taiwan. Some little nodes appeared on surfaces of their fins and skins. When the symptom became serious, they looked like sarcomas. Almost every organ, including liver, spleen, kidney, fin, heart, stomach, intestine of a hard-sick cobia were dealt through the following processes:fixation, section and staining. Only at the connective tissues of fins and epithelium did we find hypertrophy cells which own big nuclei and inclusion bodies around the nuclei.

中文摘要本实验自屏东小琉球采集某家养殖业者箱网网中的罹病海鲡,外观上,鳍与部分体表出现结节状之小颗粒,严重时,呈现瘤状肉柄,取其各部位组织包括:肝脏、脾脏、肾脏、鳍、心脏、胃、肠等进行固定、切片及染色,发现唯有於鳍与皮肤部位之结缔组织细胞,明显可见肥大之现象,肥大之细胞拥有巨大细胞核与围绕著细胞核的包涵体网状结构,由於肥大细胞之堆叠,导致鱼体之鳍与一些表皮部位有颗粒状的「结节」存在。

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