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In winter,nuclear of GTH cells were elliptical and a small number of secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季,GTH细胞核为椭圆形,有少量的分泌颗粒散布在细胞核的周围;STH细胞分泌颗粒数量较少,胞核变形,合成和分泌活动比较微弱;TSH细胞胞质中的分泌颗粒相当多。

In winter,nuclear of GTH cells were elliptical and a small number o f secretory granules scattered around nucleolus. Number of secretory granules in STH cells were relatively small, nucleolus distorted, activity of synthesis and secretion decreased. Secretory granules in TSH cells cytoplast were relatively small.

冬季,GTH细胞核为椭圆形,有少量的分泌颗粒散布在细胞核的周围;STH细胞分泌颗粒数量较少,胞核变形,合成和分泌活动比较微弱;TSH细胞胞质中的分泌颗粒相当多。

It turned out that many inclusions were found in the nucleolus and the cytoplasm of PK15 cell. The paracrystalline arrays was found in the inclusion in cytoplasm whose electron dense is deep; The nuclear of PK15 cell dissolved and there were margination of multiple chromatin aggregates and suspected intranuclear viral inclusion in PK15 cell.

细胞质中包涵体电子密度深,位于核膜周围,内有呈晶格状排列的病毒粒子,直径12±2nm;细胞核内染色质消散,在细胞核内发现多个电子密度深的、圆形或环状、由大量异染色质组成的包涵体,内有细微的粒状物质。

OBJECTIVE: To investigate a simple easy method for isolation of cell nuclei, and to provide a great quantity of active cell nuclei for studying neuronal nucleus transplantation, cell isolation-fusion and nucleus composition.

目的:探索简单易行的分离细胞核的方法,为神经元细胞核移植、细胞拆合以及细胞核成分的研究提供大量完好有活性的细胞核。

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明:百合花粉母细胞中有类似核纤层的结构存在;在染色质穿壁运动过程中,首先是细胞核向细胞壁靠拢,并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域;其后是染色质开始穿壁,在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域,到了染色质穿壁后期,大部分核物质都已穿至相邻细胞,并彼此融合,而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

Cells of which DNA contents were more than 4C were 58.4%,which indicated the existence of proliferative vigorousness.

TM上皮细胞核面积及核周长介于正常上皮细胞与癌细胞之间,与后两者相比均有显著性差异(P<0.01),反映TM上皮细胞核有一定的异型性。

The morphologic change of muscle fibers: At the early stage of denervation, the area of cross sections decreased, some muscle fibers turned into angular fibers, nuclei congregated around the center of the fibers. After 8 weeks of denervation, atrophic and hypertrophic fibers coexist. Collagen increases under the endomysium and between the fibers.

肌纤维的组织形态学变化:面部肌肉失神经支配早期,肌纤维的横截面面积变小,出现角状纤维,肌细胞核聚集,细胞核从肌纤维边缘移至肌纤维中央附近;失神经支配后期,肿胀与萎缩的肌纤维并存,肿胀的肌纤维的横截面成倍大于正常的肌纤维,而萎缩的肌纤维的横截面则成倍小于正常的肌纤维;有大量的角纤维存在,肌纤维之间有较多的结缔组织增生。

The Ca〓 in nuclear oscillated before GVBD and became stronger when Ca〓 transferred from extranuclear into the nuclear.

在GVBD发生前,细胞核内出现有规律的强度越来越大的波动现象,细胞核周围的Ca〓不断向细胞核内波动传递。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡,原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化:细胞核固缩,核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘,并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用,与对照组相比有显著性差异(p<0.01),在剂量为0.8-8μmol/L范围内,抑制率随剂量的增加而增加,当剂量超过8μmol/L时,抑制率反而下降;3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性,引起DNA降解损伤,反向调节细胞周期活动,促使细胞从G0期进入G1期,抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核,可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

Including has had the cell nucleus biological -- genome team since the first eukaryon biology from the yeast to the humanity to break the code, the scientist has wanted to know why biological majority DNA haven't formed the useful gene.

自从第一个真核生物——包括从酵母到人类的有细胞核的生物——的基因组被破译以来,科学家一直想知道,为什么生物的大多数DNA并没有形成有用的基因。

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