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RESULTS: hMSC had good homogeneousness and immunological reaction after implantation.

结果: 第5代呈集落生长的hMSC,均一性好。hMSC可在脑内存活6周以上;随着时间的延长,hMSC在脑内迁移的范围越来越大;间接免疫荧光表明hMSC在大鼠脑内表达人神经元特异性烯醇化酶、神经丝蛋白和胶质纤维酸性蛋白;模型鼠的肢体运动功能有明显改善。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别,采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞,经7天G418(600μg/ml)药物筛选,共获得30个neo阳性细胞克隆,其中12个为非绿色细胞克隆,仅有7个可以扩大培养。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型,出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点,1,2周后处死大鼠,相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型,出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点,1,2周后处死大鼠,相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚。

Results: The degenerative changes were demonstrated in the knee joints injected with papain and L-cysteine, such as the lower activity scope of knee joint, thinner and worse articular cartilage, fissuration, fibrous degeneration, fibrillation and destroyed cartilage matrix.

结果:实验侧关节注射木瓜蛋白酶后,活动范围明显低于对照侧,关节软骨变薄且有裂隙形成、纤维变性、基质分解破坏等退行性改变,并随观察时间的延长而进一步发展。

There has been the report such as the male agaric fruiting body can restrain the tumour, gallbladder fibrosis, lipide overoxidation and so on. But both here ahd abroad have no report about male agaroc mycelium amylose could decrease bllod pressure and protect liver fuction.

有关桑黄子实体多糖抗肿瘤、抗肝纤维化与抗脂质过氧化作用活性及桑黄菌丝体多糖抗肿瘤活性的研究已有报导,但国内、外有关桑黄菌丝体多糖的降血糖、保护肝功能作用等生物活性的研究至今尚未见报告。

Protophase empirical study confirmed that SLT can treat nephritis model of immunity and mesenterium hyperplasy, and can obviously lighten interstitial fibrosis of nephric tubule induced by Adriamycin.

前期实验研究证实,SLT 对大鼠免疫性系膜增生性肾炎模型有治疗作用,能明显减轻阿霉素所诱导的肾小管间质纤维化。

Eighteen (72%) chronic cases showed a pattern resembling usual interstitial pneumonia, but, in most cases, with more peribronchiolar fibrosis than one would expect in UIP.

慢性患者中有18例(72%)的组织结构类似寻常型间质性肺炎,但与UIP不同的是,绝大多数病例细支气管周纤维化病灶不只一处。

In the SMC of the normal rabbits, myofilaments and macula densa were abundant, few mitochondrla and endoplasmic reticulums were found around the nuclear, and the SMC was in the contractive stage. In the SMC of the model rabbits, few myofilaments and macula densa were found, rough surfaced endoplasmic reticulums and mitochondria were abundant, some phagocytosed much lipid and became foam cells, the SMC was in the synthetic stage.

实验12周,高脂组家兔主动脉斑块面积百分比为47.69±12.84%,内膜斑块厚度为0.68±0.08mm,内膜斑块横断面积与中膜面积比值为1.28±0.35;内膜脂质斑块弥漫且较厚,有大量的泡沫细胞堆积,可达8~10层,管腔直径变小,内膜不光滑;泡沫细胞内富含脂滴,呈椭圆形气球样,有的斑块中可见显著的平滑肌细胞增生及大量的弹力纤维形成。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。