有生物的

Totally, these results indicated that tailing pile could be the new source for finding new heavy-metal-tolerant microorganisms. Two morphologically distinct types of colonies investigated in this study may include more than 4 undescribed bacterial species.

同时还初步发现几个有进化生态学意义的现象：(1)矿渣极强的重金属选择作用和环境隔离条件可能导致新物种在短期内形成；(2)自然选择可能在物种以上层次发挥作用；(3)不同环境因子对微生物生物量、抗性细菌比例、物种和遗传多样性等生物指征的作用不同；(4)不同的生物特征对环境因子修饰作用的敏感程度也不一样。

In this thesis, we did modifications of alkyl groups upon benzyl fluorophosphonate. In order to study the influence of alkyl length and steric effect on activity and selectivity of probes, we synthesized benzyl fluorophosphonate recognition heads with modifications including ethyl, butyl, octyl and cyclohexyl. By way of connecting different kinds of tag like azido group, biotin and rhodamine with recognition heads separately through a higher hydrophilic ethylene glycol derived linker, a series of benzyl fluorophosphonate probes were made up. And they will apply to various biological testing strategies.

在本论文中，我们以苯甲基氟化磷酸酯为基础，於其上进行一系列烷基之修饰，为了探讨烷基长度与空间立体性质对於探针活性及选择性的影响，我们合成了包含乙烷基、丁烷基、辛烷基以及环己烷基修饰的苯甲基氟化磷酸酯辨识端，并藉由具较高亲水性的聚乙二醇衍生连接桥结合三种不同的发报端，分别有叠氮基团、生物素及萤光基团来构成不同的化学探针组合，这一系列苯甲基氟化磷酸酯类化学探针将可被应用於不同的生物测试策略。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

With the seismic profile and the comparison between the eustatic sea level change and sequence, the authors find that there developed different scales of reef and carbonate platforms in the sag. These reef and carbonate platforms have many developing stages which can be correlated with the eustatic sea level change and evolutionary stages, besides, they all have preferable hydrocarbon potential.

通过地震资料的解释及海平面变化曲线与层序的对比发现，琼东南盆地南部深水凹陷发育有规模大小不等的生物礁和碳酸盐岩台地，而且这些生物礁和碳酸盐岩台地的发育与海平面变化曲线和构造演化的阶段可以对应起来，应具有较好的油气勘探潜力。

The strata between the carbonate rocks of the Chaguoluoma Formation (Devonian-Early Carboniferous) and carbonate rocks of the Xiala Formation consist mainly of clastic rocks with limestone, which are marked by glaciomarine deposits in terms of sedimentation and mixing of Gondwanan and Tethyan faunas in terms of fossils.

古生物群混生的现象为石炭纪—二叠纪的特提斯生物区与冈瓦纳生物区之间的地层和古生物对比建立了一座桥梁。永珠组中上部地层中同时产有牙形石和腕足类，牙形石的研究表明其时代为晚石炭世莫斯科期，而腕足类的研究则表明其为早二叠世萨克马尔期。

Histological observation showed no vital osteocytes were found in bone lacuna and vessels perforating into Haversian canals disappeared in control group after operation.

细胞和矿化的细胞间质组成的骨组织有其独特的生物组织学特性，为人体最坚硬的结缔组织，即使其细胞成分全部坏死，仍可在一定的时期内保持其组织学结构形态，且仍具有一定的生物力学功能。

This paper focuses on the stability study of Rhizopus oryzae IFO4697 when used for the methanolysis of renewable oils for biodiesel production. The results showed that water content was important for achieving high catalytic activity and good stability of the biocatalyst. The optimum water content was found to be 5%-15%. Both particle size and desiccation methods showed no obvious effect on the stability of the biocatalyst. With GA cross-linking pretreatment, the stability of the biocatalyst could be improved significantly. When Rhizopus oryzae IFO4697 repeatedly used for next batch reaction, direct vacuum filtration was found to be a good way for the maintenance of good stability of the biocatalyst.

本实验对固定化全细胞Rhizopus oryzae IFO4697催化植物油脂合成生物柴油的稳定性进行了系统地研究，结果表明：反应体系水含量对于全细胞催化剂的反应活性和催化稳定性有重要影响， 5%~15%含水量适宜；研究范围内，载体粒度及干燥方式对稳定性影响不显著；经过戊二醛交联后，全细胞催化油脂甲醇解反应的稳定性显著提高， 1200 h反应后，仍然可以保持75%的生物柴油得率；真空抽滤直接回用的方式有利于稳定性的保持。

The increasing of soil Bray-1 phosphorus was significantly correlated to the dissolved labile phosphorus (p 0.001), and the phosphorus leaching loss under the precipitation of two-times of normal precipitation is more than 3.6 times than that of normal precipitation.

生物固形物的施用会造成表层0-10 cm土壤Bray-1磷的累积，由於土壤中Bray-1磷浓度与洗出液中溶解性反应磷的流失量有显著相关(p 0.001)，且两倍雨量淋洗下洗出液中磷的流失量是正常雨量下的3.6倍，因此，生物固形物的施用仍须考量磷流失的风险。

This particular Chinese article is an extensive account of Ginger as a medicinal plant and its biomedical research results, and is full of classical Chinese medical quotes from doctors who lived in eras from the Tang Dynasty (more than 1,000 years ago) to the Qing Dynasty. Several native English speaking translators turned down the job because of the difficulty of the archaic language and the frontier biomedical research.

这是一篇中医百科全书中关于生姜的文章，是对生姜作为中药及其现代生物医学研究成果的详细描述，其中包括从唐朝以来著名中医对生姜的论述，由于涉及到医学古文和生物医学最新研究的内容，几位母语为英语的美国翻译都觉得太难而推辞了，其中有一位接了下来，但是翻译了8000多字后，由于做不下去而不做了。

Guangzhou-yan-Biotechnology Co., Ltd. is a set of health care products beauty technology research, development, production, marketing, legal advice, professional training is one of the limited liability company, located in the scenic mountain clouds, a very good academic research Atmosphere and the well-advanced information network and a number of master's degree, graduate students graduated from China Medical University and the pharmaceutical engineer.

广州燕香生物科技有限公司是一间集生物保健品美容技术研究、开发、生产、销售、相关法律咨询、业务培训等于一体的有限责任公司，公司地址位于风景秀丽的白云山下，有很好的学术科研氛围及先进灵通的信息网络，并拥有多名硕士、研究生及毕业于中国医科大学的制药工程师。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。