有生物的

There is a secret in the well-growing, the master of tea plant Lijiang Sun said that last year 4000 chicken were raised on the hillside next to tea plant, so all the fertilizer used in the plant was dejecta of the chicken, which is a kind of organic fertilizer, containing many necessary nutritious elements for plants growing.

茶叶特别好是有奥秘的，茶园的主人孙利江说，去年茶园边上的山坡上养了4000余只本鸡，而茶园里所有的肥料都来自本鸡的粪便，鸡粪是有机肥料之一，含有多种作物生物必需的营养成分，所以，这里的茶叶可是实打实的生态茶叶。

And you're responsible for it, what of your MAN, who is always the erected, the vitalized inorganic, the latest of the ephemera, the creature of temperature strutting his little space on the thermometer.

是从你那里来的，就从你的那个'人'来的，那个永远直立的、被激活了的无机物，蜉蝣的最新形式，在温度计那小小的天地望高视阔步的有体温的生物。

My Office is located in Zhejiang Province Tianmushan University, has rich forest resources, particularly resources are particularly rich pecan, pecan is a kind of epicarp environmental protection, low-cost coverage of a new type of garden materials, and its contains a biochemical factor that can effective in the soil to kill pests; and can increase the green color, rich green texture, landscaping, green landscape and green land; to prevent dust and soil erosion, improve air quality, so that a more pleasant environment; reduce transpiration, especially in summer, be conducive to maintaining soil moisture, prevent soil knot board, to maintain temperature, saving water, at the same time to effectively control weeds.

我处位于浙江省的天目山系，有丰富的森林资源，特别是山核桃资源尤为丰富，山核桃的外果皮是一种环保、价廉的新型园林覆盖资材，其含有一种生物化学因子，能有效的杀死土壤中的害虫；并且能增加绿地色彩，丰富绿地质感，美化、绿化园林绿地；防止扬尘和水土流失，提高空气质量，使环境更优美；减少蒸腾，特别是夏天，有利于保持土壤水份，防止土壤结板，保持温度，节约水源，同时能有效的控制杂草生长。

Another matter has something to do with the genetical theory of hair loss attaching a rumor that it is just enough to have a look at a father and you'll see his son when he gets older, which is not biologically correct as assumes the sole influence of father's genes in forming son's cells.

另一个问题是有可能与遗传理论脱发附上一份传闻，这只不过是足以看看父亲，你会看到他的儿子当他变老，这是不正确的假设生物的唯一的影响的父亲的基因在形成儿子的细胞。

Pine wilt disease is one of the most important pine tree diseases, and its causal agent is Bursaphelenchus xylophilus, which is a great threat to the pine forestry in many countries such as China Japan, Korea, the US, Canada, Mexico, and Nigeria, etc. It is one of the most important quarantine organisms, and also listed on A2 in China, which lead to imponderable loss every year.

松材线虫病是一种重要的林业病害，向来有"松树的癌症"的称号，对松树的危害十分严重，它的病原是松材线虫，它是包括日、韩、美、加拿大、墨西哥、尼日利亚和我国等许多国家重要的林业病害，也是许多国家的检疫对象，在我国属于二级检疫危害生物，造成的损失难于估计。

The analysis, led by Tim Berra, professor emeritus in the Department of Evolution, Ecology and Organismal Biology at the Ohio State University in Mansfield, found that Darwin's kids did have "a moderate level of inbreeding" and in the family's children, there was "a significant positive association between child mortality and inbreeding."

分析工作由位于Mansfield的俄亥俄州立大学进化、生态和有机生物部门的荣誉退休教授Tim Berra主持，分析发现达尔文的孩子们的确受"中等程度近亲繁殖的影响"，并且在家族的儿童中"儿童死亡率和近亲繁殖有显著的、确定的联系。"

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

So far, the cancerogenic mechanism of lung cancer is not well elucidated. Some growth factors and growth factor receptors have been identified to participate in cancerogenic processes, but the downstream pathways regulated by those growth factor receptors are uncleared.

目前已知的癌症发病机理多是生长因子和生长因子的表面受体发生异常所致，因此，进一步阐明受体下游的调控基因对于阐明癌症的发生机理和进一步设计药物靶位点都是十分有意义的。forkhead box P4(FoxP4)就是本文作者在利用生物信息学方法筛选人类心脏发育候选基因的过程中发现的一个人类新基因，目前发现三种转录本。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。