有液泡的

Calcium antimonite deposits, which indicates calcium distribution, were mainly localized in cellmembrane and vacuoles, as a storeroom of Ca2+, and smaller amounts of calcium p recip itates randomly resided in mitochon2dria, cytop lasm and nucleus.

结果表明，对照生长条件下(14 mmol·L-1 NO3-)，黄瓜根系分生区细胞内Ca2+主要出现在细胞膜和小液泡内，线粒体、细胞质和细胞核及核仁内也有少量的较小颗粒钙沉淀。

Under normal temperature.the Ca2+-ATPase activity was found on the innerside of the PM.on the vacuole envelope and the chloroplast envelope,and theenzyme activity declined upon heat stress.The Ca2+-ATPase activity on the vacuoleenvelope,PM.and the chloroplast envelope in both Ca2+-and oxalate-treated peppermesophyll cells was higher than that in the control under the same condition.TheCa2+-ATPase was also found at the granum lamella,and it was activatedsignificantly by exogenous Ca2+ and oxalate treatments.Under heat stress.the Ca2+-ATPase activity declined slowly in both Ca2+- and oxalate-treated mesophyll cells.But La3+ treatment inhibited the enzyme activity under both normal condition andheat stress.

常温下生长的叶肉细胞，在质膜、液泡膜、叶绿体被膜等处有Ca2+-ATPase活性，热胁迫后酶活性下降；外源Ca2+和草酸预处理对辣椒叶肉细胞各种膜上Ca2+-ATPase活性具有促进作用，特别是定位于液泡膜、质膜和叶绿体被膜上的酶活性明显比对照提高；在叶绿体基粒和基质片层上也有酶活性，并且Ca2+和草酸预处理对该部位上的酶活性激活作用更明显；La3+处理的作用与Ca2+和草酸处理的效果相反。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuo le membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中，质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物，线粒体和小泡上只有少量酶活性反应产物。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuole membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中，质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物，线粒体和小泡上只有少量酶活性反应产物。

We can see the cells whose cytoplasms are thick and have very distinct nucleus, but it have not the central vacuole.

这些细胞的细胞质稠密有明显的细胞核，但是没有中央液泡，其泌盐过程主要是靠细胞的破裂来完成。

" Vacuole: Space within a cell that is empty of cytoplasm, lined with a membrane , and filled with fluid."

液泡：细胞质内以膜包绕并充有液体的空间。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明，未经低温处理的董棕幼苗叶肉细胞，焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀；经2℃ 48 h低温处理后，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少，并且超微结构已初步显示出寒害的特徵，叶绿体外膜部分破损，类囊体片层稀疏且排列不规则，光合速率明显下降等；经2℃ 120 h低温处理后，细胞间隙内的焦锑酸钙沉淀极少，有的也紧贴在细胞外壁上，而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀，在核基质和液泡中也可见到少量的焦锑酸钙沉淀，并且超微结构遭到了显著破坏，叶绿体结构完全被破坏，核膜与液泡膜严重破损，内部结构模糊，细胞只表现为呼吸作用，不进行光合作用。

The situation of abnormal development of male cells is as follows:microspore mother cell can't enter into meiosis because of intense vacuolation,shrink and disintegration of its cytoplasm;although vacuolated microspore mother cell can enter into meiosis,it can't form normal dyad and degenerate in the middle process;dyad and tetrad become vacuolated and can't develop normally;cytoplasm of microspore shrinks around the nucleus at the stage of central nucleus microspore,the shape of microspore is twisted into crescent or irregular shape,at last its cytoplasm and nucleus are disintegrated and crescent vacant microspore presents;nutritive substances can't be accumulated at the stage of vacuolated microspore,cytoplasm is disintegrated,and microspore turns into a big vacant pollen.

雄性细胞异常发育有几种情况：小孢子母细胞强烈液泡化，细胞质收缩解体，不能进入减数分裂；小孢子母细胞液泡化，虽能进入减数分裂，但不能形成正常二分体而中途退化；二分体、四分体细胞液泡化，不能进行正常发育；单核小孢子中央期，细胞质收缩包围核，小孢子形状扭曲呈月牙形或不规则形，最终细胞质和核解体而呈月牙形的空壳小孢子；单核液泡期的小孢子不能积聚营养物质，细胞质解体而成为大的空壳花粉粒。

Using tamato seedlings as experimental material, we examined the adaptive changes of the proton translocating pumps and protein components in vacuolar membranes of plant during phosphate starvation.

本项目以番茄为材料，对磷饥饿时植物液泡膜质子ATP酶和焦磷酸酶活性以及液泡膜蛋白组分的适应性变化进行研究，以探明磷饥饿时植物液泡膜质子泵活性的变化与Pi跨液泡膜运转的关系，并了解磷饥饿时植物液泡膜是否产生有特异性诱导蛋白及其可能的功能。

Under optical microscope,deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue.However,there was no granular protein in the development season barks.

经汞—溴酚蓝染色后的石蜡切片显微观察表明：休眠期枝条细胞的液泡中有大量蓝色小球状蛋白颗粒，有的甚至充满整个液泡，而在生长期枝条细胞中则未发现这种蛋白颗粒的存在。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。