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The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法,制备出具有生物活性的蛋白质毒素,构建了白喉毒素表达载体pET22b-DT,并转入大肠杆菌BL21(DE3)中诱导表达,以融合表达的His-6作为标签通过亲和层析纯化;表达毒素经蛋白酶酶切后,形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素,并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Encephalopathia alco-holica may have 5 patterns in imageology. Brain MRI has a high diagnostic value in encephalopathia alcoholica.

酒精中毒性脑病在影像学上可以有5种不同的表现形式,颅脑MRI检查对酒精中毒性脑病具有较高的诊断价值,B族维生素及营养支持对治疗有效。

Results show that, following the leaching time extending, the leaching concentrations of heavy metals and lixiviate toxicity of heavy metals increase in the fly ash. When the ratio of liquid and solid add, the leaching concentrations of heavy metals obviously become big, and the Cd's leaching concentration is the biggest, when the ratio of liquid and solid is 90, the Cd's leaching ratio exceed the percent of 70. At the time pH is about 7, the leaching concentrations of heavy metals are the lowest. When the grain size is the bigger or little, the leaching concentrations of heavy metals are lower, however when the grain size is 250-900 μm, the concentrations are higher. After fly ash melt in the high temperature, the lixiviate toxicity of heavy metals greatly reduce, which explains that the effect of melting on heavy metals' stabilization is very good.

结果表明,随着浸出时间的增加,飞灰绝大部分重金属的浸出浓度增高,浸出毒性增强;液固比增加,大部分重金属的浸出率大大提高,其中重金属Cd的浸出率最大,在液固比为90时,飞灰中的Cd的浸出率达到70%以上;pH为中性左右的时候,医疗垃圾飞灰中的大部分重金属的浸出液浓度最低;颗粒尺寸在较大或者较小时,医疗垃圾飞灰中的大部分重金属浸出浓度较小,大部分重金属在颗粒尺寸250~900 μm时,重金属的浸出浓度较高;飞灰高温熔融后,重金属的浸出毒性大大降低,说明熔融对重金属有很好的固化效果。

The results are: 1 the stricture of the capillaries of stria vascularis was observed in most cochleae of rickety rats and, segmental deletions of the outer hair cells on the first turn of cochlea were found in a small number of rickety rats; 2 the perilymphatic ionized calcium concentration significantly re- duced and the threshold of CAP (Compound Action Poten- tials of the eighth nerve) significantly rose in rickety rats in comparison with those of normal control; 3 gentamicin results in an increase of the perilymphatic ionized calcium concentra- tion in rickety rats; 4 the cochleae of the rickety rats appeared to be less vulnerable to the ototoxicity of gentamicin than the normal one.

研究包括两个方面,其一是&佝偻病的感觉神经性耳聋及其对庆大霉素耳毒性作用的耐受性&,系用大白鼠建立维生素D缺乏性佝偻病动物模型,发现:(1)佝偻病大白鼠耳蜗血管纹毛细血管狭窄,少数动物耳蜗底回外毛细胞有节段性缺失;(2)佝偻病大白鼠的耳蜗鼓阶外淋巴钙离子浓度降低,CAP(听神经复合动作电位,以下简称CAP)听阈提高;(3)庆大霉素可使佝偻病大白鼠耳蜗外淋巴钙离子浓度上升;(4)佝偻病大白鼠耳蜗在功能和形态上都比正常者更能耐受庆大霉素的耳毒性损害。

objective to search the factors of liver injury via the anit-induced liver injury in rats.methods male sprague-dawley rats,were divided into 4 groups,data were analyzed using a completely randmized factorial anova and linear correlation.results after anit intoxication,the necrosis of hepatic cell and neutrophil infiltration im periportal region of the liver were observed in liver setions.jinshisan can reduce the alt,the tbil.pla2 correlated positively with alt、akp and tbil.conclusion the severity of anit-induced liver injury correlated positively with the pla2.jinshisan can protect against the rat anit-induced liver injury.

目的 拟通过对α-萘异硫氰酸酯诱导大鼠肝损伤发病机理的探讨,对引起肝损伤的因素进行系统的研究。方法选择s-d大鼠,雄性,随机分4组,检测数据以平均值±标准差表示,采用方差分析,相关分析进行统计学处理。结果s-d大鼠经anit染毒后,病理切片显示肝损伤明显,汇管区有大量中性粒细胞浸润。经金石散及地塞米松治疗后,生化指标均有所下降,中药金石散优于地塞米松,血生化指标与pla2活性相关分析显示,呈正相关。结论 anit诱导的肝毒性损伤程度与肝组织匀浆pla2活力呈正相关,应用抑制剂降低pla2活力,肝损伤程度也减轻,中药金石散具有降低pla2活性,减轻肝毒性损伤。

T show evidence of toxicity between the verapamil treated animals and control animals.conclusionsubconjunctival verapamil decreased the incidence of tractional retinal detachment due to traumatic proliferative vetreoretinopathy in this rabbit model.verapamil at the dose used in this model has no evident toxicity on rabbit eyes.further studies are needed to determine the doseresponse and efficacy of the drug.

结果在模型制备后第35天,治疗组18只眼发生牵引性视网膜脱离,占56.3%,对照组26只眼发生,占81.3%(χ2=4.655,p=0.031);玻璃体视网膜增生程度治疗组较对照组明显减轻(秩和检验,u=3.5419,p<0.001);未发现ver对角膜上皮有明显毒性反应。结论结膜下注射ver能有效抑制外伤性pvr的发展,减少牵引性视网膜脱离的发生率;ver在实验所用剂量对角膜上皮无明显毒性反应。

Ligustrazine hasn't significant toxicity effect on the nasal mucosa when perfusing in nasal cavity, but synthetic borneol has.

川芎嗪在鼻腔灌流时对鼻黏膜无明显的毒性影响,而冰片在鼻腔灌流时对鼻黏膜则有显著性的毒性影响。

The toxicity to the liver were found in 27.8% and skin rashes appeared also in 27.8% of the patients. Eleven point one percent of the patients had different degrees of alopecia and toxicity of oculi, while the toxicity to the heart was only 2.78%.

结果27.8%的出现肝损害,2.78%的患者出现心脏毒性,11.1%的患者出现眼毒性,27.8%的患者出现皮肤过敏,69.4%的患者出现消化道反应,11.1%的患者有不同程度的脱发,36例患者均无肾功损害。

Eleven point one percent of the patients had different degrees of alopecia and toxicity of oculi, while the toxicity to the heart was only 2.78%. No toxicity to kindey appeared in all the patients involved.

结果27.8%的出现肝损害,2.78%的患者出现心脏毒性,11.1%的患者出现眼毒性,27.8%的患者出现皮肤过敏,69.4%的患者出现消化道反应,11.1%的患者有不同程度的脱发,36例患者均无肾功损害。

Results Reactions of digestive tract such as nausea and vomiting occurred in 69.4% of the patients. The toxicity to the liver were found in 27.8% and skin rashes appeared also in 27.8% of the patients. Eleven point one percent of the patients had different degrees of alopecia and toxicity of oculi, while the toxicity to the heart was only 2.78%.

结果27.8%的患者出现肝损害,2.78%的患者出现心脏毒性,11.1%的患者出现眼毒性,27.8%的患者出现皮肤过敏,69.4%的患者出现消化道反应,11.1%的患者有不同程度的脱发,36例患者均无肾功损害。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。