有斑点

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Evergreen shrub or small tree whose foliage is conspicuously blotched with red and yellow and having small black fruits.

常青灌木或小树，它的叶子带着明显的红色和黄色的斑点，有小黑果实。

Corolla campanulate; petals 5, yellow , with or without dark purple basal spot.

花冠钟状；花瓣5，黄，有或没有基部的深紫色斑点。

A brightly colored fish of the family Chaetodontidae of warm seas, having a laterally compressed body.

豹纹蝶蛱蝶科的各种蝴蝶，尤指史培蝶属和boloria，褐色翅膀背侧有黑或银色斑点

In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV. The results showed that WSSV positive cases were detected in copepoda from egg to adult, and the positive copepoda appeared earlier than cultural shrimp in ponds.

1。本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵，以及由这些休眠卵孵化出的幼体携带病毒情况，发现桡足类由卵到成体各阶段中均有阳性个体，而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。

Any of various birds of the family Corvidae found worldwide , having a long graduated tail and black , blue , or green plumage with white markings and noted for their chattering call .

喜鹊遍布世界各地的属于鸦科的一种鸟，长有分层的长尾，羽毛呈黑色，蓝色或绿色并带有白色斑点，以其叽叽喳喳的独特叫声而著称。

As he went lower, he saw that it spread among small promontoriesand inlets of rough, sharp rock, and the crisping, lapping surface showed stains斑點 of purple and darker blue.

越往下走，他发现它的四周有很多小岬和满是又尖又粗糙的岩石的水湾，被波涛拍打的易碎的表面上显出紫色和深蓝色的斑点。

Thepatientsshouldbegivensynthesizedtherapyincludinginvadedmechanicalventilate,correctedhypoxidosis,high-dosehormoneandantibioticsandcomplications,whenthepatientswithseverechesttraumaappearprogressiveemptysis,dyspnea,cyanopathy,andstainsorpiecesofhigh-densityshadowinchestX-ray.

严重胸外伤患者如出现进行性咯血、呼吸困难、发绀及肺部斑点状或片状高密度阴影要考虑到严重肺挫伤并发急性呼吸窘迫综合征的可能，应早期进行有创机械通气、纠正低氧血症、大剂量激素和抗生素及并发症治疗等综合治疗措施。

Any of numerous tropical marine gastropods of the genus Cypraea having highly polished usually brightly marked shells.

宝贝属的多种热带海生腹足动物，壳非常亮且通常有鲜艳的斑点。

Leaves simple, rosulate, margin roundly dentate; raceme terminal; flower ca. 1 cm long, light purple, the upper lip 2-lobed, the lower lip 3-lobed, larger and yellow spotted; stamens 4, in two pairs of two different length (= didynamous); fruit a capsule.

单叶大多於基部丛生，叶缘钝锯齿状；总状花序顶生；两性花约1公分长，淡紫色，上唇瓣二裂，下唇瓣3裂且较大，内侧有黄色斑点；雄蕊4枚，2常2短；蒴果。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。