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In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素,该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分;对毒素蛋白成分鉴定结果表明蛋白条带多,每种蛋白的含量少,小分子量蛋白含量多,糖蛋白染色结果显示,毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白,分子量小于35.8kDa的蛋白多为非糖蛋白;性质鉴定结果显示,毒素中有活性的蛋白成分具有部分可耐高温高压,且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

Because of the crustal structure in the Central US which efficiently propagates seismic energy, shaking from earthquakes in this part of the country are felt at a much greater distance from the epicenters than similar size quakes in the Western US.

由于地壳结构,在美国中部有效地宣传地震能量,摇头,从地震在这部分的国家认为,在一个更大的距离震中较类似规模的地震是在美国西部。

The most effective solution would be to issue jointly and severally guaranteed eurobonds to refinance, say, 75 per cent of the maturing debt as long as Greece meets its targets, leaving Athens to finance the rest of its needs as best it can.

最有效的解决办法应该是:只要希腊实现了指标,就发行共同及各自担保的欧元债券,为75%的到期债务再融资,再由希腊政府尽其所能完成其余部分的融资。

The results were as follows:the mass fractions of the mineral nutrient elements in a part of the orchard plants were out of balance,especially the mass fractions of some trace elements such as B, Cu, Fe in the plants were higher; in the soilsof most orchards, the problems such as stronger acidity, higher mass fractions of readily available P, lowermass fractions of exchangeable base and lower mass fractions of available trace elements werefound.

结果表明,部分果园植株矿质营养元素质量分数存在失衡现象,尤其是植株的B、Cu、Fe等微量元素质量分数偏高。大多数果园土壤存在土壤酸性强、速效P质量分数高、交换性盐基质量分数低的问题,较多的果园土壤微量元素有效态质量分数偏低。

The findings indicate that the training method of trunk flexors and trunk extensors combined trunk rotators is more effective in stroke patients.

依据脑卒中患者躯干肌力低下的特点,选择性地强化对部分躯干肌的训练,即在加强前屈和后伸肌群训练的同时,增加躯干旋转肌群的训练,可以更加有效地提高对脑卒中患者平衡功能障碍的训练效果。

Q3 and the PNP BJT (bipolar-junction transistor), Q1, effectively buffer the two SDA/SCL lines so that no extra current flows into the open-collector and -drain stages of the I2C devices that connect to the bus when they hold the lines down.

Q3、PNP BJT和Q1有效的缓冲SDA/SCL两条线,所以当连接总线的开集和开漏部分I2C设备的线持续为低时,没有额外的电流流进。

Results are as follows:(1)To develop high yield green grass and to change grass for fish is a beneficial way to deal with the shortages of grains and concentratedfeed.

本研究根据上海精养鱼塘的生产现状,经3年(1992~1995年)大面积试验实施,指出种植高产饲草代替部分精料,以草换鱼是解决饲料不足和我国粮食短缺的有效途径。

At the theories part, this paper studies the flange plate effective width of composite truss.

在理论部分,研究了组合桁架的翼缘有效宽度。

The problem of component in heating furnace and improvement measure in detail is introduced.

介绍了加热炉部分构件存在的问题及改进措施,改进后有效地满足了生产的需求。

The heat conductivity calculation has been divided into two parts, form the borehole to the exposure of cement mantle and in the formations, as for corresponding to different borehole structures, different methods have been adopted to sum the overall heat transfer coefficients and numerical method is used to calculate the heat loss on each nodal point, furthermore, through the model proposed herein, the degrees of influences of different borehole structures on heat loss could be calculated in order to get the most economic and efficient borehole structure.

把导热分为井筒到水泥环外壁和地层导热两部分计算,对不同的井筒结构采用不同方法计算总传热系数,数值法求解每个节点处的热损失,并通过模型对比计算了不同井筒结构对井筒热损失的影响程度,得到最经济有效的井筒结构。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特,那个是爱朗。

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明确的宗旨,目标和重点战略业务计划。