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Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its

在进行了重组质粒的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后,实验取得了令人满意的结果,证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白,同时也说明pcDNA3-VEGF165重组真核表达质粒是有效的。

This article precedes with the general methods of the validity of policy enforcement, summarizing the means of policy enforcement, the criteria of the validity of policy enforcement, and the factors which prevent the policy enforcement.

文章总结了公共政策的执行手段、公共政策有效执行的标准和影响公共政策有效执行的因素。

By analyzing the bode diagram of HAPF system and minimizing the capacity of HAPF, the paper presents a frequency dividing control method based on fuzzy-PI. In this mean generalized integrator is used for frequency dividing integral and fuzzy arithmetic is for adjusting PI coefficients timely. Compare with other kinds of control methods, the proposed one is more simple and easier realization.

该文由常用的并联型HAPF的结构特点分析出发,从容量和幅频特性2个方面探讨HAPF控制中进行有效分频的必要性,根据HAPF对控制方法的要求,文章提出一种基于广义积分的模糊自整定PI控制方法,即通过广义积分实施对周期量的分频积分,同时由模糊算法进行比例和积分系数的在线调整,从而有效实现对HAPF的分频控制。

MJ and geraniol all could inhibit the PLBs growth. With the raising of their concentration , their inhibition inhanced. MJ 100mol L-1 could inhance the accumulation of effective components in PLBs, but geraniol at different concentration inhibited the accumulation of effective components in PLBs.

MJ和牻牛儿醇均会抑制拟原球茎的生长,随着浓度的增加,这种抑制作用增强。100μmol/L~(-1)MJ可以提高拟原球茎中有效成分的积累,而不同浓度的牻牛儿醇都抑制了有效成分的积累。

Say true and effective, exact website content is the base that Lai Yisheng of a website retains so, contain the literal information of keyword of true, accurate core especially, it is the soul of website content strategy more, a true and effective keyword, surpass those gewgaw picture news far, can be the food that your website survives and source motivation, because this pair of websites extend operation,having the action that hold the balance and enormous sense.

所以说有效、真实准确的网站内容是一个网站赖以生存的基础,尤其是含有真实、准确核心关键词的文字信息,更是是网站内容策略的灵魂,一个真实有效的关键词,远远胜过那些华而不实的图片信息,会是你网站存活的食粮和源动力,因此这对网站推广运营有着举足轻重的作用和巨大的意义。

There is no significouldt variation between the function of Method 1 and 3 in the indexes of digestive and absorptive function,except the effect on body weight and food intake;as well,model 2 shows no significouldt influence on the indexes besides body weight lose.1.4 ConclusionsContrast the three modeling methods,model 3 is the most obvious and steady method for the establishment of deficiency of spleen YANG.2 comparison on the efficacy of Lizhong Pills influenced by dried ginger and its different components on digestive and absorptive functions and energy metabolism2.1 ObjectivesThis research was designed to observed the different influences of Lizhong Pills formula on the models,which contains dried ginger or its main group of components(volatile oil and water solubles).It would be explored from two levels of material base,single herb dried ginger or its different components,to confirm that the whole effects of Lizhong Pills do mainly depend on its main herb dried ginger in the formula just as the traditional acknowledgement in TCM.Further more,the connotative meaning of dried ginger used as the main herb in Lizhong Pills egthe aspect of pharmacology and chemistry would be preliminary revealed.2.2 MethodsEach group of model rats of deficiency of spleen-YANG were to be duplicated by method 3 for 14 days,and be treated by corresponding drug samples for 8 days.

造模方法1对消化吸收相关药理指标的改变与造模方法3的影响相似,但对模型动物的体重和食量的影响不明显;造模方法2除能使模型动物的体重减轻外,对其他各指标均无显著影响。1.4结论对比实验采用的三种石膏-知母的给药方法,方法3的造模效果最为显著和稳定,即以每日灌胃给药,连续12天,恢复9天,是本实验证实的最有效的脾阳虚证候模型制备方法。2干姜及其不同有效部位对理中丸消化吸收功能及代谢的影响2.1目的通过对理中丸中君药干姜,以及干姜中挥发性成分和水溶性成分对理中丸主要功效影响的研究,从药味和化学成分群两个层次,证实君药干姜及干姜中的主要活性成分群对理中丸整体功效的决定性影响,以初步揭示理中丸中君药核心地位的药理学和化学内涵。2.2方法采用石膏-知母复合造模法制备大鼠脾阳虚证候模型,造模期为14天1;治疗期间各给药组分别给予治疗药物,连续给药8天。

On the basis of questionnaire of Garvin, Edmondson and Gino (2008), we tailored the measurement of this study. Using exploratory factor analysis, we analysis the data of 215 persons in 95 family business firms. Using confirmatory factor analysis, we analysis the data of 377 persons in 151 family business.

在Garyin和Edmondson等的组织学习问卷的基础上,结合本研究目的,形成家族企业的组织学习问卷,对95家家族企业的215份有效问卷进行探索性因素分析,对151家家族企业的377份有效问卷进行验证性因素分析。

These include the department of the amount of wrinkles, yu weiwen, glabella wrinkles, lower profile, nasolabial fold pattern.results300 cases of patients with botulinum toxin type a treatment effect were 234 cases (78%), 66 cases were effective (22%), 0 case was null and void.conclusionsa-botox in the treatment of facial wrinkles precise and effective,simple,easy,painless,non-impact,easy-to-promotion.

其中包括额部皱纹、鱼尾纹、眉间皱纹、下睑纹、鼻唇沟纹。结果:300例患者行a型肉毒毒素治疗后显效者234例(占78%),有效者66例(占22%),无效者为0例。结论:a型肉毒毒素在治疗面部皱纹确切有效,操作简便、易行,无痛苦,不影响工作,易于推广。

This method is very effective, almost all brands of machines such treatment, can effectively prevent oxidation Goldfinger memory.

这种方法很有效,几乎所有的品牌机都如此处理,可以有效的防止内存的金手指氧化。

To develop the most efficient system the golfer needs to understand how to most efficiently use their time.

为了发展最有效的系统的球员需要了解如何最有效地利用时间。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力