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Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16~23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16~23周有产前诊断指征者经腹部抽取羊水,用Chang培养液传代培养,常规制备羊水细胞分裂中期染色体,应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针,与培养的羊水细胞分裂中期染色体DNA进行原位杂交,杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16~23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16~23周有产前诊断指征者经腹部抽取羊水,用Chang培养液传代培养,常规制备羊水细胞分裂中期染色体,应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针,与培养的羊水细胞分裂中期染色体DNA进行原位杂交,杂交后用荧光显微镜观察玻片并摄像。

By 666 B.C. the process of casehardening was in use for the edges of iron tools, but the story that the Egyptians had some secret means of hardening copper and bronze that has since been lost is probably without foundation.

大致公元前666年。表面硬化方法用于铁工具刃口,但是埃及人的传说有部分秘密,意指铜和青铜的淬火,没有根据可能工艺已经失传了。

"Our scouts have managed to destroy their catapults," one soldier said, pointing at a map."So we have at least one advantage."

&我们的侦察兵已经设法破坏了它们的投石机,&一名士兵指着地图说,&至少我们现在有一个优势。&

There are certain types of diamond cuts which refer to its shape.

有某些类型的钻石削减,是指它的形状。

He demolishes the theory that we have reached 1)the "end of history", 2)the dogmas of secular liberalism, 3)the weaknesses of financial casino capitalism and 4)the limits of energy-intensive economic growth.

我之前考虑过这种断句,但是忘了reach有发现答案的意思,所以没想明白;此处可能是指Gray认为人们并没有真正明白&缺点&和&极限&,这就与他的思想比较吻合了

To be shaken by an earthquake denotes that you will have some serious business challanges.

以不可动摇的一次地震是指你将有一些严重的业务

Legitimating tactics involve efforts to verify the legitimacy of a request and the agent's authority or right to make it.

合法的策略是指:努力去证实代理人当局的要求之合法性或者证实他有权利去这样做。

The process of intentionally mismatching the maturities of assets and liabilities by borrowing short and lending long.

指根据对利率未来趋势的预期,刻意使资产与负债的长短期限有明显差距,借此牟取利益。

According to article 25 of the criminal law of China:A joint crime is an intentional crime committed by two or more persons jointly;A negligent crime committed by two or more persons jointly is not to be punished as a joint crime;those who should bear criminal responsibility are to be punished separately according to the crimes they have committed.

我国刑法明文规定:&共同犯罪是指二人以上的共同故意犯罪;共同过失犯罪,不以共同犯罪论处。&这一方面在立法上承认有共同过失犯罪行为,另一方面又不确认其为共同犯罪,这与现实情况及共同犯罪的法理极不相称。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了

The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.

世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。