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Results :①The MVs were formed by osteoblast of the secret type、dentinoblast and process;② There was a mature process of the MVs in the extracellular matrix.The appearance of the hydroxyapatite was a major mature feature;③ HAP formed initially in the MVs entered into collage matrix and eventually mineralized;④ The shifting sign of HAP from MVs to the surface of collage fibril was observed.

结果:①MVs是由分泌型成骨细胞、成牙本质细胞及其突起芽生进入胶原基质;②MVs在细胞外胶原基质中有一个逐渐成熟的过程,并以羟基磷灰石(hydroxyapatite,HAP)晶体的出现为其主要特征;③HAP在MVs内形成、增多,最终进入胶原基质并进一步沉积、钙化;④部分胶原原纤维表面沉积的HAP 晶体与MVs分泌的晶体有明显的移形迹象。

There were more fibroblasts, the collagen increased in model control group. After 30 days, the fibroblasts of model control group were hyperemic, scattered red blood cells could be found, and capillaries were proliferated. Fibrous tissue arranged in rows in the acupotomology intervention group and the blockade control group.

造模后30 d模型对照组成纤维细胞增生,可见红细胞散在分布,有增生的毛细血管;针刀干预组与封闭对照组可见纤维组织排列成行;针刀干预组较其他各组胶原纤维形成减少,瘢痕面积小。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P<0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P<0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示:(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性,而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P<0.05);三组动物的支气管和肺组织常规细菌培养结果均为阴性;感染组动物透射电镜检查见肺泡间隔增宽,其中有较多胶原纤维堆积,其余两组则未见明显异常。

RESULTS The degenerative changes of nerve ending in Meissner's corpuscles were observed after 1 month of denervation, and the basic structure of the corpuscles had no obvious changes. After 3 months, the axons of corpuscles were disappeared, and the volume of corpuscles was shrunk. The basic structure of nerves was disappeared, and the lemmocyte and neurolemma plate were changed after 5 months.

结果 失神经1个月时触觉小体内的神经末梢已有溃变,触觉小体的基本结构无明显改变;3个月时轴突消失,触觉小体的体积开始萎缩;5个月时神经结构消失,膜细胞及其膜板亦开始改变;8个月时触觉小体内胶原纤维含量逐渐增多;12个月时触觉小体内膜结构与膜间基质完全消失,被胶原纤维代替。

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象,线粒体少,胞核畸形,胞内有吞噬颗粒,细胞周围胶原纤维较少TEM×5200

The degrees of fibrosis of sternomastoid muscle in CMT are related to the hyperplasy of collagen type IV.

病变组中纤维型组IV型胶原较肌肉型组有更多的表达,两者比较有显著性差异(P.01),纤维型和肌肉型组与对照组比较均有显著性差异(P.01)。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明,处理后4周,全周角膜缘上皮板层手术切除,中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化,未发生睑球粘连,角膜基质胶原纤维完整未见溃疡、穿孔等病变,细胞印迹学检查为结膜表型,可作为实验性角膜缘干细胞移植的病理模型;全周角膜缘上皮板层手术切除,中央角膜上皮用生理盐水擦除的5只试验家兔,有2只为结膜表型,另3只为角膜表型,观察期内结果不稳定;半周角膜缘上皮板层手术切除,中央角膜上皮层用生理盐水擦除的5只试验家兔,角膜表面透明,全部为角膜表型;直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔,有4只角膜基质胶原纤维断裂、溶解,并伴有严重的溃疡、穿孔、睑球粘连等病变,不能用于移植试验,另1只角膜表面透明,未见结膜和新生血管长入,细胞印迹学检查为角膜表型。

In this article, the properties of the synthesized fibers including the rmicrocosmic appearance, the thermodynamic stability and so on were investigated and tested, and their swelling capabilities were also studied. The results show that the decompounded temperature of the synthesized fiber is increased by about 100K and the cross-linked fibers have good swelling capability in solvents and non-solvents.

本文还对合成后纤维的微观形貌、热稳定性等性质进行了考察与测试,对其溶胀性能进行了初步研究,结果表明合成后纤维发生热分解的温度要比原纤维高100K左右,在良性和非良性溶剂中都有很好的溶胀性能。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果:①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后,逐渐呈灰黄色,表面粗糙,出现裂隙;当压力逐渐减小后,变黄、粗糙、有裂隙的软骨颜色逐渐恢复,变得有光泽②光镜下可见表面光滑、平整,细胞分布均匀、整齐,层次清楚的软骨在关节面压力增大后,表面变粗糙、缺损,细胞排列紊乱、染色不均;当关节面压力逐渐减小后,软骨表面逐渐修复,细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达,术后3周内表达逐渐变弱,从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后,软骨细胞逐渐变扁,胞质中内质网膜、高尔基体减少,胶原排列紊乱;当关节面压力减小,软骨细胞形态逐渐恢复正常,胞质中内质网膜、高尔基体数量逐渐恢复;胶原纤维排列逐渐有规则。

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