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有丝分裂原

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Soon after mitosis, a procentriole is elaborated perpendicular to the parental centriole.

很快在有丝分裂之后,原中心粒被垂直地分裂成四个中心粒。

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

IGF2, which encodes a protein functioning as a fetal growth factor and a cell mitogen, is expressed only from paternally allele in most tissues but from both alleles in adult liver. H1 9, which acts as an RNA, is a gene of unknown function and expressed only from maternally allele.

IGF2编码的胰岛素样生长因子-2是一种胚胎生长因子及细胞有丝分裂原,在人体内大多数组织中特异地表达父系来源的等位基因,而母源等位基因处于印记状态。H19表达一种功能尚不清楚的RNA,其印记状态与IGF2相反,目前发现H19在正常组织内均为母源等位基因表达,父源等位基因印记。

The fern spore, the original cell of the gametophyte, carries out asymmetrical mitosis to produce a large cell and a small cell. The small cell geminates to a rhizoid by tip growth, and the large cell further divides and develops to become a prothallus.

蕨类植物孢子作为配子体的原始细胞,通过不对称的有丝分裂产生一大一小两个细胞,小细胞萌发出极性生长的假根,大细胞继续分裂发育为原叶体。

Mitogen-activated protein kinase cascade and maturation-promoting factor play very important roles during meiotic maturation and fertilization of oocyte.

目的探讨有丝分裂促进因子和丝裂原活化的蛋白激酶在小鼠受精卵第一有丝分裂期中的作用及相互关系。

Detect with Western Blot protein of MAb counterpoise form and the natural phosphoric acid is enzymatic reactivity in the cell, obtain 6 individual plant to be able to be stabilized in all secrete the individual plant of cross tumour cell that fights phosphoric acid enzymatic MAb, go enzymaticly to study double particularity phosphoric acid further phosphation action mechanism and its activation formerly in mitosis way of enzymatic signal of albumen phosphoric acid turns guide provide strong tool.

用Western blot检测mAb对重组蛋白和细胞中天然磷酸酶的反应性,共获得6株可稳定分泌抗磷酸酶mAb的杂交瘤细胞株,为进一步探究双特异性磷酸酶的去磷酸化功能机理以及其在有丝分裂原激活蛋白磷酸酶信号途径中的信号转导提供强有力的工具。

The testis index, testis volumes were same as the annual changes of testis mass. The curves of annual variation were all unimodality.2 The spermatogenetic cycle of Myospalax cansus comprises seven stages with significant features: Stage I , from February to April, the testis were at the stage of spermatogonia proliferation. In this period, testis index and the number of spermatogonia began to rise. Other spermatogenic cells had not yet formed; Stage II to III, from March to April, primary spermatocyte meiosis period. The testis index was highest in this stage, and spermatogenic cells were in spermatocyte stage, the primary spermatocyte meiosis generated to secondary spermatocyte; Stage IV, from April to May, spermatocytes continued to split, germ cells appeared in seminiferous tubules; Stage V, in May, sperm formation, spermatids of seminiferous tubules were transformed to spermatozoa, a large number of sperms existed in the lumen; Stage VI, spermatozoa emission period, from May to June, testis index were a significant drop and mature spermatozoa excluded gradually; VII, the testicular activity ceased basically from July to September, November to January of the following year, the spermatogenic activity ceased completely. Therefore, Myospalax cansus are animals of seasonal reproduction, spermatogenesis cycle is discontinuous type.

睾丸系数、体积和重量的年周期变化规律一致,变化曲线呈现单峰型。2甘肃鼢鼠雄性生殖腺的年周期活动由7个特征明显的时期构成:Ⅰ期,2~3月份,精原细胞增殖期,睾丸系数开始上升,精原细胞进行有丝分裂,其他生精细胞尚未形成;3~4月份为Ⅱ~Ⅲ期,初级精母细胞成熟分裂期,睾丸系数达到最大,生精细胞大多处于精母细胞阶段,初级精母细胞减数分裂生成次级精母细胞;Ⅳ期,4~5月份精母细胞继续进行分裂,精细胞在生精小管内出现;Ⅴ期,5月份,精子形成期,曲细精管中精细胞变态成精子,在管腔中存在大量的精子;Ⅵ期,精子排放期,5~6月份,睾丸系数显著下降,成熟精子从生精小管上脱离,逐渐排除;Ⅶ期,精原细胞停滞期,7~9月份睾丸生精活动基本停滞,11~翌年1月,生精活动完全停止。

Blood samples were drawn at 30 minutes before and 0 minute, 12 hours, 24 hours, 36 hours and 48 hours after the race from 14 subjects' forearm veins. PHA and LPS were used for assessing lymphocyte proliferation; lyses of 51Cr labeled K562 target cells were used for assessing natural cytotoxicity. Results showed that the lymphocyte proliferation stimulated by PHA and LPS was decreased at least for 24 hours; the natural killer cytotoxicity is increased at 0 minute and decreased at least 48 hours after the race.

本研究以14位一般大学男生为研究对象,血液样本於14位受测者跑步前30分钟及跑步后0分、12小时、24小时、36小时与48小时於受测者前手臂静脉抽取,以PHA及LPS有丝分裂原刺激淋巴细胞,评估周边血液中淋巴细胞增殖功能;以自然杀手细胞分解51Cr标记之K562标的细胞释出51Cr之总量,评估自然杀手细胞毒杀活性。

Objective To study on the function of mitogen- activated protein kinase and mitosis- promoting factorin the first mitosis of mouse fertilized eggs and the relationship between them.

目的探讨有丝分裂促进因子和丝裂原活化的蛋白激酶在小鼠受精卵第一有丝分裂期中的作用及相互关系。

Besides, they play important roles in the regulation of mitogenic signal transduction and cell cycle control in response to extracellular stimuli.

此外,它们还在有丝分裂原激活蛋白磷酸酶信号途径中起重要生理功能。

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