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B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.
体细胞胚胎发生研究的结果表明:(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料;(2)在所试验到的MS、MS1/2(将MS的所有成分均减半)、WPM、DKW、B〓、SH等六种基本培养基中,MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基;(3)水曲柳的体细胞胚胎发生以直接发生为主,体细胞胚主要来自于从合子胚分离的完整子叶;(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响,诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2,5,10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0,2mg/L+TDZ 0.05mg/L;(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力,少数愈伤组织可以分化出体细胞胚;(6)愈伤组织的诱导也受培养基和激素配比的显著影响,最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2,5mg/L+TDZ0.05mg/L;(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力,此时种子尚未成熟,胚乳已呈固态,种胚的各个部分已分化完全,种胚体积占胚腔的大约2/3;(8)在各自综合的最适条件下,完整子叶的体细胞胚诱导率可达33.68%,下胚轴的愈伤组织诱导率可达90.7%。
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Value is the product of the relationship of demand and supply between subjects and objects Subjects consist of three levels such as human totality,groups subjecting to the human totality,and human individuals Coresponding to it,objects include the outside world with respect to human totality(groups+individuals+the world outside human being),the outside world with respect to human groups,and the outside world with respect to human individuals Therefore,things possess value,so do human beings Because humans themselves are the unity of subjects and objects,humans have selfvalue The reciprocal function of the factors constituting value decides the production of value,promote the changes of value,which is the basic subject of thelaw of value The three factors which have influence on the changes in value are the demand of subjects.the character of objects.and practice The origin of the conflicts of value concepts lies in the differences and opposites of the living conditions of human subjects The direct originis the differences and opposites of value objects Therefore,in order to gradually eliminate the conflicts of human value concepts,the differences of the living conditions of human subjects must be eliminated thoroughly
价值是主客体之间需要与满足关系的产物。主体有人类整体、人类整体之下的群体以及人类个体三个层次,与之相应,客体也包括与人类整体相对的外部世界(群体+个体+人以外的世界)、与人类群体相对的外部世界以及与人类个体相对的外部世界。因而不仅物具有价值,人亦有价值。由于人自身是主客体的统一,因而人还有自我价值。构成价值的各个要素相互作用决定价值的生成、推动价值的变化,这是价值规律的基本内容。影响价值变化的主要有主体需要、客体属性及实践三个要素。价值观念冲突的最终根源在于人类主体生存条件之差别和对立,直接根源则在于价值客体的差别和对立。因而要逐步消除人类价值观念的冲突就必须从根本上消除人类主体生存条件方面的差别做起。
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Article 6 To obtain a work safety license, an enterprise shall satisfy the following work safety conditions:(1)Having established and improved the responsibility system for work safety,and formulated a whole set of work safety regulations and operating rules;(2)Its investment In safety Is up to work safety requirements;(3)Having set up administrative entities for work safety and installed full-time work safety administrative personnel;(4)The major person-in—charge and work safety administrative personnel have passed the appraisal;(5)The special personnel have passed the appraisal conducted by the competent authority, and have obtained qualification certificates for special operations;(6)The workers have gone through work safety education and training;(7)Having workers insured against work—related injuries in accordance with the law and having paid insurance premiums in this regard;(8)Its premises,worksites,safety facilities,equipment and technology are up to the requirements of the relevant work safety laws,regulations,standards and rules;(9)Having preventive measures against occupation hazards and providing workers with labor protection articles which are up to the national standards or standards of the industrial sector concerned;(10)Having conducted safety evaluation in accordance with the law;(11)Having measures for the testing,assessment and monitoring of sources of grave danger, as well as emergency plans thereabout;(12)Having emergency rescue plans for work accidents,and entities or personnel specialized in emergency rescue.and having necessary emergency rescue materials and equipment; and (13)Satisfying other conditions as provided by laws and regulations.
第六条 企业取得安全生产许可证,应当具备下列安全生产条件:建立,健全安全生产责任制,制定完备的安全生产规章制度和操作规程;设置安全生产管理机构,配备专职安全生产管理人员;主要负责人和安全生产管理人员经考核合格;主要负责人和安全生产管理人员经考核合格;特种作业人员经有关业务主管部门考核合格,取得特种作业操作资格证书;从业人员经安全生产教育和培训合格;依法参加工伤保险,为从业人员缴纳保险费;厂房,作业场所和安全设施,设备,工艺符合有关安全生产法律,法规,标准和规程的要求;有职业危害防治措施,并为从业人员配备符合国家标准或者行业标准的劳动防护用品;依法进行安全评价;有重大危险源检测,评估,监控措施和应急预案;有生产安全事故应急救援预案,应急救援组织或者应急救援人员,配备必要的应急救援器材,设备;法律,法规规定的其他条件。
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Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.
结果 在培养30天内,条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成;而2×105个/ml组和1×105个/ml组都有钙化结节形成,而且有细胞越少形成结节越早,数量越的趋势;给予条件培养液又较常规培养液培养有结节形成早而多的趋势。
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Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group cnpared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early cnpared with the DMEM medium.
结果 在培养30天内,条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成;而2×105个/ml组和1×105个/ml组都有钙化结节形成,而且有细胞越少形成结节越早,数量越多的趋势;给予条件培养液又较常规培养液培养有结节形成早而多的趋势。
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Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.
结果 在培养30天内,条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成;而2×105个/ml组和1×105个/ml组都有钙化结节形成,而且有细胞越少形成结节越早,数量越多的趋势;给予条件培养液又较常规培养液培养有结节形成早而多的趋势。
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Study result suggest: The change of stock in RFCCU directly affect gasoline content. Gasoline content can be reduced through increasing the equilibrium activity of catalyst, controlling reaction level, increasing recirculating rate of gasoline and stability of gasoline level. Conclusions drawn from experiment is that optimization of operating condition has great effect on lowering olefine content of gasoline which can be reduced from 60% to 50%.Reducing reaction temperature is useful for lowering olefine content of gasoline, when the reaction temperature is raised, thermal cracking rate is higher than catalytic cracking rate, so that olefine content is increased. Raising catalyst-to-oil ratio is good for lowering olefine content. With conversion rate increased, the yield of LPG and coke is raised, the yield of gasoline is increased firstly and then decreased slightly, hydrogen transfer index is increased, so the olefine content is decreased.
研究结果表明:催化裂化装置的原料的变化直接影响到催化汽油的烯烃含量;可以通过优化催化装置的操作条件来降低催化汽油中的烯烃含量,其中通过提高催化剂平衡活性、控制反应深度、增加汽油回炼量、汽油深度稳定可以降低催化汽油烯烃含量,优化工艺条件对降低催化汽油烯烃含量有较大作用,汽油烯烃含量由60%降至50%左右;降低反应温度有利于降低催化汽油的烯烃含量,当反应温度的提高,热裂化反应速度提高的幅度大于催化裂化反应速度提高的幅度,汽油烯烃含量增加;剂油比的提高有利于降低催化汽油的烯烃含量,转化率提高,液化气产率提高,汽油收率先增加后略有下降,焦炭产率增加,氢转移反应指数提高,汽油烯烃含量下降;反应时间对产品分布及汽油烯烃含量有影响,增加提升管反应时间,液化汽、汽油产率提高,干气和焦炭产率增加。
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All materials must be available to others. This means that commercial sources must be identified, proprietory materials must be patented prior to publication, and all cultures, strains or varieties on which the work is based must be deposited in an identified culture collection from which they can be obtained by others.
那个实验当然可以重复,不过每个实验都有它必要的条件,这个实验的条件首先就是要有相信实验可以成功的人,因为心念是有力量的可以改变水的微观结构,其实当年很多关于气功的实验已经多次证明了,只是后来被不理智的抹煞了。
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In view of these, the second part of this paper presents two sufficient conditions and two mixed type duals for the generalized fractional programming only under-convexity assumptions. These sufficient conditions apply to a broader class of mathematical programming problems.The results about weak duality, strong duality and strictly reverse duality arealso presented under more suitable conditions.
鉴于此,本文的第二部分,我们仅在函数—凸性假设下,给出了广义分式规划的二个最优性充分条件,这些充分条件较文献中的相关的条件有更广泛的适用性;同时还给出了混合型对偶,并且在适当的条件下,给出了相应的弱对偶定理、强对偶定理,以及严格逆对偶定理。
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Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.
确定了双亲原生质体形成与再生的最佳条件:菌株F46、SC1采用二次菌丝培养,即在蔗糖含量20%菌丝培养液里,25℃条件下150 rpm培养48 h;然后按15%的接种量转接到蔗糖含量30%,甘氨酸含量分别为1%、1.5%的菌丝培养液中,同样条件下,继续培养48 h;在34℃条件下,菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1,菌株SC1用10 mg·mL-1溶菌酶,分别酶解3 h、2.5 h,不仅有大量的原生质体形成,且再生率也高。
- 推荐网络例句
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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.
此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。
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To get the business done, I suggest we split the difference in price.
为了做成这笔生意,我建议我们在价格上大家各让一半。
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After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.
一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。