最近部分

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

In a recent usability session, ten out of ten users looked right past a prominently-displayed Help button, choosing instead to 'satisfice' their way through the tasks they were asked to perform.

在最近的可用性部分，10个用户全部都忽视一个显示分明的&帮助&按钮，反而选择用自己&满意&的方式去操作。

Fisher and her colleagues have conducted recent fMRI scans of people who are not just in love but newly in love and have found that their ventral tegmental areas are working particularly hard.

最近，Fisher和她的同事们对那些不但是相爱而且是新近相爱的人们做了功能磁共振成像扫描，发现腹側被盖区部分&工作量&尤其大。

Recently we have held regular business card printing and membership card when making staff training, part of the Enterprise Manager, Business card printing and membership card factory workers and cultural level is not high enough, it is very difficult to understand business card printing and membership card making professional training content, lacking the necessary basic knowledge of theory, and the overall cultural workers better low ... in business card printing and membership card making industry professional skills training, in-charge of the city, is actually run by the Indian enterprises said: glad to see the we run a machine of the workers are illiterate, write his own name uncompletely!

最近我们协会举办定期的制卡和会员卡制作职工培训时，部分企业经理反映，制卡和会员卡制作厂的工人文化水平不够高，很难理解制卡和会员卡制作专业培训的内容，缺乏必要的理论基础知识，而且职工的整体文化水平逐年走低……在制卡和会员卡制作业内组织专业技能培训时，有的民营印企负责人竟然推脱说：我们这开机器的职工都没文化，自己的名字都写不全呢！

In a word, mass! Recently undeliberated NB was popular, like old saying of making all clear without mention a word, using Beijing dialect it's much easier --"B-Pretending", represented by part small star blogs and strange name blogs, more over the writing is, the better it can up to people's demands, just like Taiwan entertainment programs, but it cannot be formal, as it's not necessary to collect and publish a book. Talk about Taiwan entertainment programs I had to mention Jacky Wu, this man who was characterized by A use his own power saved many programs, and it's really funny. Recently I always view "People in the Pan", it stands for another Taiwan entertainment character -- imitation, it makes me laughing a lot, and I can refer a sentence from Dong Lu's blog "If Li Yong comes to Taiwan he must lose his job." Fortunately ordinary family cannot see these Taiwan TV Stations, or CCTV cannot defend its market share, in fact it's forced by a loose competitive atmosphere, the fact that Hunan TV is so hot now in China reminds me what an ancester said: a kid became famous only because of there is no hero in world.

这两 年流行的网络文字是不经意间的NB，就像古话说得&不着一字尽得风流&，拿北京话说就简单多了叫&装B&，以部分小明星的博和名字怪异的为代表，文字越潇洒或者越俗越能迎合大家的需求，就像台湾的娱乐节目，不过上不了台面，如果硬要出个书什么的我看大可不必，说起台湾娱乐节目，不能不提吴宗宪，这个活跃舞台几十年的以A为特点的全才中年男子以一己之力养活了很多节目，而且确实很好笑，最近我看的比较多的是全民大闷锅，代表台湾娱乐的又一大特点－－模仿，常常能让我前仰后合，不由想到董路 BLOG上的一句话，&如果让李咏去台湾肯定会失业&，还好现在台湾的这些台普通家庭还看不到，不然CCTV一定招架不住，其实说白了都是一个宽松的竞争环境逼的，湖南卫视现在在大陆这么火，让我想到一句话：世无英雄，遂使竖子成名。

Insurance: People familiar with the situation said insurance companies had mostly underweighted equities during the recent rally, due to their cautious stance.

保险：知情人士透露，由于采取保守的投资策略，保险公司在最近的股市暴涨中大部分&踏空&行情。

But China in recent weeks has waged an aggressive public relations campaign on the issue of hacking, apparently at least in part aimed at discrediting allegations from Google Inc.

但最近几周，中国在黑客问题上发起了声势浩大的宣传行动，显然至少有部分原因是为了消除谷歌Google Inc。

The Mitchell system recently added a PDR component to their estimating software, further legitimizing PDR and making it easy for insurance companies to agree on pricing.

米切尔系统最近增加了一个民主共和国部分软件的估计，进一步合法化共和国和便于保险公司商定价格。

How do you handle the most difficult aspects of your current job....

你是如何处理你最近目前工作中最困难的部分的？

Question: How do you handle the most difficult aspects of your current job?

你是如何处理你最近目前工作中最困难的部分的？

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。