最后一次

Now we cannot forget the last email, the last conversation, the last meal together, the last au revoir.

我们怎能忘记他们发出的最后电邮，与他们的最后交谈，与他们的最后聚餐，与他们最后一次道别。

Both teams, on the last or only free throw, a jump ball situation occurs.

由双方球队在最后一次或仅有一次的罚球，一次跳球情况发生

But I have not yet given up all hope of finding some way of hastening the approach of sleep. Even yet there is a glimmer, for rereading (not for the first, and, please Heaven! not the last time) Lamb's letters, I came upon the following, in a note to Southey; But there is a man in my office, a Mr. H., who proses it away from morning to night, and never gets beyond corporal and material verities!...

7但我对能找到某种方法加速睡眠的到来并未完全放弃希望，甚至还有一线微光，因为重读(不是第一次重读，并且要是可能，也不会是最后一次)兰姆的书信集时，在他给骚塞的一封短信中，我读到下面一段：可我办公室有一个人，一位H先生，他整天从早到晚写啊说啊，但从不超出肉体与物质现实的描绘！

Results: this a 19 years old patient with moderate differentiated adenocarcinoma of right seminal vesicle. the tumor recurred twice locally after local excision of the tumor combined with radiotherapy and prostatovesiculectomy. he was eventually cured by radical excision of bladder with indiana pouch. the patient is still alive without recurrence more than 10 years after the last operation.

结果：1例发病年龄19岁的右精囊中分化腺癌病人，先后经过肿瘤局部切除术加放疗和前列腺精囊切除术，两次治疗后肿瘤均局部复发，最后又行膀胱全切，indiana膀胱术后治愈，病人从最后一次手术至今已接受随访10年以上，至今无复发仍存活。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

It's easy enough to say that man is immortal simply because he will endure, that from the last ding-dong of doom has clanged and faded from the last worthless rock hanging tideless in the last red and dying evening, that even then, there will be one more sound: that of his puny and inexhaustible voice still talking.

说人类之所以不朽仅仅是因为他能忍受，说即使厄运的最后一次钟声在再也没有潮水冲刷的、映在末日最后一抹余晖里的最后一块无用礁石旁渐渐消失时，还会有一个声音，那就是人类微弱但不倦的声音，再继续说这些都很容易。

And lastly, in this, that alike the first and the last of His Utterances begin with 'Father,' does He show by the unbrokenness of His faith and fellowship the real spiritual victory which He has won.

最后，在这方面，这都第一次和最后一次他的话语开始的父亲，并不表明他的unbrokenness自己的信仰和研究金的真正精神胜利法，他韩元。

After a flurry of ciaos, 4,000 misdirected passes and a good old fashioned handshake, my passing had improved from utterly hopeless to merely below average."It's all about technique and concentration," Pirlo explained. I spent years working at my game to get to where I am.

为了对比，有必要做一些自我介绍，我在12岁那年被学校足球队除名了，因为我在9场比赛中打进了4个球，可惜进球方向是朝着自己的球门，我最后一次踢出过顶传球击中了我奶奶的晾衣绳，然后飞进了邻居的温室，经过无数次被轰退场的激励、4000次无目的传球和一次次善意的老套握手，我的传球水平终于由不可救药提高到了比平均水平差一点点的程度。

In contrast, I was dropped from my school team at 12 for scoring four own goals in nine games and the last time I hit a lofted pass it deflected off my grandmother's washing line and broke my neighbour's greenhouse. After a flurry of ciaos, 4,000 misdirected passes and a good old fashioned handshake, my passing had improved from utterly hopeless to merely below average."It's all about technique and concentration," Pirlo explained.

为了对比，有必要做一些自我介绍，我在12岁那年被学校足球队除名了，因为我在9场比赛中打进了4个球，可惜进球方向是朝着自己的球门，我最后一次踢出过顶传球击中了我奶奶的晾衣绳，然后飞进了邻居的温室，经过无数次被轰退场的激励、4000次无目的传球和一次次善意的老套握手，我的传球水平终于由不可救药提高到了比平均水平差一点点的程度。

It was indeed the first last and only dance that I ever had with my father.

确实，这是我第一次、最后一次、也是唯一一次和我的父亲跳舞。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。