最佳

Taking Carica papaya and celery as main materials,the processing technique for the mixed drink and its stability have been studied throug h orthog onal test 、sense assess and microbiolog ical analysis in this paper.

以番木瓜、芹菜为主要原料，采用正交设计、感官评价和微生物检验等方法，筛选了复合饮料的最佳配方，探讨了其稳定性，确定了番木瓜芹菜复合果蔬汁饮料的最佳工艺。

Results indicate that the optimum extraction conditions are as follows: ultrasonic power is 600W, temperature is 53℃, the ratio of liquid to solid is 8︰1, ultrasonic treating time is 70min, reflux extraction time is 120min, the extraction rate of oleanolic acid is 3.000 mg/g; XDA-1 resin is favorable in purification of OA in Chaenomeles sinensis Koehne, the optimum purification conditions are found to be 0.794 mg/mL of OA concentration, the flow rate of adsorption is 0.5 mL/min; the desorption solvent is 90% ethanol, and the flow rate of elutant is 1.0 mL/min.

结果表明，超声波辅助提取最佳工艺条件为：超声功率600 W、提取温度53℃、液料比8：1、超声波提取70 min，回流提取120 min，齐墩果酸得率为3.000 mg/g；XDA-1型大孔树脂对齐墩果酸具有较好的吸附和解吸效果，最佳纯化工艺条件为上柱液浓度0.794 mg/mL，吸附流速0.5 mL/min；以90%浓度乙醇洗脱，洗脱流速1.0 mL/min，纯化后得提取物中齐墩果酸纯度可达26.55%。

Chapter two, the preparation of liposomes in DPPC/Chol/DPPA mixed system were investigated systematically for the first time. The optimal conditions for preparation and the composition of liposomes were established, the encapsulation efficiency was up to 50.4%.

第二章 首次系统研究了反相蒸发法用于DPPC/Chol/DPPA混合类脂体系的脂质体的制备，确定了脂质体的最佳制备条件和最佳组成，获得了高达50.4％的包裹效率。

A chemical coagulatory coprecipitation method was used in the treatment of blast-furnace gas washing water.

用化学混凝共沉淀的方法处理高炉煤气洗涤水，实验确定了最佳的药剂为聚合氯化铝钙，工艺的最佳操作条件是聚合氯化铝钙加入量为33mg/L，PAM加入量为0。

A chemical coagulatory coprecipitation method was used in the treatment of blast-furnace gas washing water.

用化学混凝共沉淀的方法处理高炉煤气洗涤水，实验确定了最佳的药剂为聚合氯化铝钙，工艺的最佳操作条件是聚合氯化铝钙加入量为33mg/L，PAM加入量为0.5mg/L，搅拌时间为60s，搅拌强度为90r/min。

The results showed that N6+TDZ0.05 mgL^(-1)+NAA0.01 mgL^(-1)+KT0.50 mgL^(-1) was most suitable for Platanthera mandarinorum with an induction rate of 92.5%; N6+TTDZ0.05 mgL^(-1)+KT0.50 mgL^(-1) was most suitable for Coeloglossum viride with an induction rate of 98%; N6+IAA0.01 mgL^(-1) was most suitable for Platanthera mandarinorum with a germination rate of 96%; N6+IAA0.01 mgL^(-1)+GA30.01 mgL^(-1) was most suitable for Coeloglossum viride with a germination rate of 94.5%.

结果表明，最适合长白舌唇兰原球茎和类原球茎诱导的培养基为N6+TDZ0.05mgL^(-1)+NAA0.01 mgL^(-1)+KT0.50 mgL^(-1)，诱导率为92.5%；最适合凹舌兰原球茎和类原球茎诱导的培养基为N6+TDZ0.05 mgL^(-1)+KT0.50 mgL^(-1)，诱导率为98%；长白舌唇兰类原球茎萌发为完整植株的最佳培养基为N6+IAA0.01 mgL^(-1)，萌发率为96%；凹舌兰类原球茎萌发为完整植株的最佳培养基为N6+IAA0.01 mgL^(-1)+GA30.01 mgL^(-1)，萌发率为94.5%。

This paper analyses the coupling wastage in columniform wave guide gas laser,and gives the function between the coupling wastage of laser guide modulo EH11 and the position and radius of reflector.

分析了圆柱形波导气体激光器中的耦合损耗，给出了最低阶波导模EH11的耦合损耗与反射镜位置、曲率半径的函数关系。研究表明：反射镜的位置和半径存在着最佳值，在取得最佳值时，可得到最低的耦合损耗

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

MTT assay was adopted to determine the inhibition rate for selecting the best effect time and concentration of Crocin.

采用MTT法测定不同浓度及不同时间藏花素对T24细胞抑制率，选出最佳药物浓度和最佳作用时间。

The reverse transcription polymerase chain reaction RT-PCR and cDNA microarray were used to evaluate VEGF-C mRNA expression of T24 cells at around the best effect time and as the Crocin concentration reached the best.

用RT-PCR和基因芯片分别检测最佳作用时间和最佳药物浓度作用前后T24细胞VEGF-C的表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。