最佳

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

But the real idea behind The Boxmasters was both musically and in live performance, it was like watching a combination of 60's television shows like Dean Martin, Johnny Cash Show, all those variety shows, with electric hillbilly music - it's a great blend.

TOTO也凭借这张专辑于当年的葛莱美奖评选中大出风头，一举囊括六项大奖，分别是：年度最佳唱片、最佳和声编排、最佳伴奏乐器编曲、年度最佳专辑、最佳录音工程以及年度最佳制作人奖。

And also we analyzed the best time of exposition, the best time of deposition, the best COD concentration of the water from the infall during the period of SBR method, and meanwhile, analyze the result.

分析了SBR工艺阶段的最佳曝气时间、最佳沉淀时间、最佳进水COD浓度等，并对其结果进行讨论。得出最佳曝气时间为8 h，最佳沉淀时间为2 h，最佳进水COD浓度在310 mg/L左右。

BK-52. The optimal conditions were found to be 30°C, pH 7.0, fermentation time 36 h, car-bon source of saccharose, inorganic nitrogen source of ammonium sulfate and enzyme inducer of disodium cis-epoxysuccinate.

最佳发酵条件为：最佳温度30°C，最佳pH7.0，最佳发酵时间36 h，最佳碳源蔗糖，最佳无机氮源硫酸铵，最佳酶诱导剂顺式环氧琥珀酸二钠。

Something really great that happened today (5/4) is that my dear little peggy was nominated for tw's 金曲獎最佳專輯製作人獎 along with the great uncle bean (who produced all of my self-composed songs), and of course, Tizzy bac for their album.

今天（5/4）有大事件发生就是我亲爱小PEGGY和陈挥斌叔叔（制作我所有自己写的歌曲的人）一起提名台湾金曲奖最佳制作人奖。

The film was passed to a Intel lectual disturbance person the descr IPT ion of life has reflected every aspect of u.s.a.s life, important incident of social political life make and represent to these decades such as u.s.a.

1995年的第六十七届奥斯卡金像奖最佳影片的角逐中，影片《阿甘正传》一举获得了最佳影片、最佳男主角、最佳导演、最佳改编剧本、最佳剪辑和最佳视觉效果等六项大奖。

By setting the retting duration of 20 hours, the factors affecting the residual gum percentage of the retted kenaf fibers are studied. The experimental results show clearly that the following factors have the significant influence on the residual gum percentage of the retted kenaf fibers and show the obvious variation tendency. The preferred processing parameters are as follows: pH value 7. 0、bath ratio 1: 50、reuse percentage of retting liquid 50%、air flow volume 2. 5L/min、temperature 40℃、nitric additives (ammonia is better than nitrates) percentage 0.45%.By comparison with anaerobic retting, the retting duration using aerobes is much shorter than that using anaerobes.

确定了以好氧微生物脱胶作为本课题的脱胶路线后，在特定的脱胶时间（20小时）条件下，通过分析不同参数条件下的残胶率，研究了好氧微生物脱胶的各种影响因素对红麻脱胶的影响规律，并优选出最佳脱胶液回用比为50％，最佳pH值为7.0，最佳气流量为2.5L/min，最佳浴比为1：50，最佳水浴温度为40℃，最佳含氮添加剂为铵盐类化合物，最佳含氮添加剂用量为0.45％。

Jennifer passed away, and their son have been to go to school age. Gan to send his son on a school bus, sitting in the bus stop bench, recalled the experiences of his life.

阿甘正传》获1995年第67届奥斯卡最佳影片、最佳男主角、最佳导演、最佳剧本改编、最佳剪辑、最佳视觉动机六项大奖。

L 11.24 Bolt, Isinbayeva win awards 博尔特伊辛巴耶娃获年度最佳 Jamican sprinter Usain Bolt and Russian pole vaulter Isinbayeva won the IAAF World Athlete of the Year awards Sunday in Monte Carlo, Monaco.

当地时间23日晚，国际田联在摩纳哥蒙特卡洛将2008年度最佳男女田径运动员分别授予牙买加短跑选手博尔特和俄罗斯撑杆跳选手伊辛巴耶娃。

By means of these conditions, computer searches out many quasi-perfect and double quasi-perfect binary array pairs with small sizes. The composite construction methods for quasi-perfect and double quasi-perfect binary array pairs are studied.

研究了准最佳和双准最佳二进阵列偶的复合构造法；并利用最佳、准最佳和双准最佳二进阵列偶之间的联系，给出了构造最佳二进阵列偶和准最佳二进阵列偶的方法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失