最优条件

Fermentation time, carbon content and inoculum influence to the liquid state fermentation were studied.

实验通过发酵培养时间，碳源含量，接菌量对液体发酵产木聚糖酶的影响分析，得出产木聚糖酶的最优条件，即在加入50 Mandels液体培养基，28℃条件下，底物含量为0.045g，接种量为20ml，培养36h。

The microwave-assisted extraction and Florisil solid phase extraction conditions were optimized. Under the optimum condition, the recoveries of 18 organochlorine pesticides (α-HCH,β-HCH,γ-HCH,δ-HCH, heptachlor, aldrin, heptachlor epoxide, endosulfan Ⅰ, p, p'-DDE, dieldrin, endrin, endosulfan Ⅱ, p, p'-DDD, o, p'-DDT, endrin aldehyde, endosulfan sulfate, p, p'-DDT, methoxychlor) were

优化了微波萃取和固相萃取柱净化条件，在最优条件下18种有机氯农药（α-HCH、β-HCH、γ-HCH、δ-HCH、七氯、艾氏剂、环氧七氯、硫丹Ⅰ、p，p'-DDE、狄氏剂、异狄氏剂、硫丹Ⅱ、p，p'-DDD、o，p'-DDT、狄氏剂、异狄氏剂醛、硫丹硫酸酯、p，p'-DDT、甲氧滴滴涕）的回收率在

A substrate concentration of 0.277 kg/L, enzyme/ substrate level of 1408 U/g, temperature of 39.2 ℃, and a hydrolysis time of 4.86 hour were found to be the optimum conditions for high antibacterial activity. Under this condition, the bacteria Escherichia coli, Micrococcus luteus and Bacillus subtilis in the hydrolysates had the inhibitory rate of 71.40%, 56.88%, and 64.54%, respectively.

经Design Expert 7.1.2软件优化，得出胃蛋白酶酶解鲶鱼骨蛋白的最优条件为：底物浓度0.277kg/L，加酶量1408 U/g，水解温度39.2℃，水解时间4.86 h，在此条件下获得的水解物，对大肠杆菌、藤黄微球菌及枯草芽孢杆菌的抑菌率分别为71.40%、56.88%和64.54%。

The pectin enzymolysis technology,juice debittering technology,and alcohol and acetic acid fermentation were studied. The results showed the best conditions of pectase hydrolysis are:amount of pectase 0.02%,temperature 50 ℃,pH 4.4 and time 4 h.

结果表明：果胶酶水解的最佳条件为：果胶酶添加量0.02%、温度50℃、pH4.4、时间120min；果汁脱苦最佳条件为：粒状活性炭添加量0.5%、室温搅拌60min；酒精发酵的最优条件为：接种量8%、发酵温度30℃、糖度20%、pH4.4、发酵时间4d；醋酸发酵的最优条件为：接种量10%、温度34℃、酒度8%、时间4d。

In connection with the character of Boc L-Glutamic Acid and aminoacetic acid structures, the reaction of aminoacetic acid acylated via Boc Anhydride has been studied on the condition of CH3OH in the paper. The yield of the derivatives is characterized by the melting point, the content of carboxylgroup and the product purity. The results showed that the best experiment condition is followed.

本论文以甲醇作为均相反应介质，Boc酸酐与谷氨酸为原料，在碱性条件下，以产物的熔点，羧基含量以及产物纯度作为产物质量确定的指标，最终得出N-Boc-谷氨酸的合成最优条件。

The effects of four factors (concentration and the quantity of NaOH,temperature and heating time) on the protein recovery were studied on the base of the analysis of mycelium residue of itaconic acid fermentation.

研究了衣康酸工业化生产中所产生的土曲霉废菌丝体的主要组成成分，用碱液提取的方法进行了蛋白质的提取回收实验，考察了NaOH溶液浓度、用量、加热温度及时间对菌丝体脱蛋白效果的影响，结果表明衣康酸菌体脱蛋白的最优条件为：NaOH液浓度0.5mol/L，碱液用量为菌丝体的40倍，90℃条件下加热9h。

The single factor tests and orthogonal experimental designs were applied to optimize the extraction conditions for the crude polysaccharides from Marasmius androsaceus.

通过单因子和正交实验确定安络小皮伞菌丝体粗多糖提取的最优条件为：料水质量体积比1∶30、提取温度70℃、提取时间2h、提取次数1次，在此条件下粗多糖的提取率达到11。

In order to investigate the electrotransformation condition of Sinorhizobium meliloti variable curve was devised by different cell cycles time,resuscitative buffer,electrotransfection buffer,pulsed electric field and resistance.

为了研究苜蓿中华根瘤菌电转化的条件，通过对不同的细胞生长周期、复苏缓冲液、电转缓冲液、电场强度和电阻对电转率影响程度来作出变化曲线，以确定电转化的最优条件，从而提高苜蓿中华根瘤菌电转化率。

Firstly, headspace solid-phase microextraction was established to determine AITC by orthogonal design. Extraction temperature, stir speed, extraction time, and desorption time were set at 40℃, 900r/min, 30min, and 5min, respectively. Under these optimal conditions, volatile flavor compounds in mustard oil, wasabi, and 'Chongcai'leaf mustard product were identified by SPME/GC/MS. In these three samples, 6,12,17 constituents were identified respectively; Allyl isothiocyanate was the main flavor compound, which accounted for 73.45%, 64.25%, 32.74% of total volatile compounds, respectively. Carbon disulfide, a hydrolysis product of AITC, accounted for 14.37% 25.40%, 51.67% of total volatile compounds respectively. There were some other isothiocycannates, such as butane, 1-isothiocyanato-, isobutyl isothiocyanate, 1-benzene, 4-isothiocyanato-, benzene (2-isothiocyanatoethyl)-, etc.

首先采用正交实验设计优化固相微萃取技术的前处理条件，结果表明，以萃取温度40℃、搅拌速度900r／min、萃取时间30min、解吸时间5min为最佳条件；在此最优条件基础上，利用SPME／GC／MS联用技术分析芥末油、青芥辣、冲菜的挥发性风味成分，分别检出6、12、17种化合物，主要风味物质均为AITC，相对丰度分别为73.45％、64.25％、32.74％；AITC的水解产物二硫化碳的相对丰度分别为14.37％、25.40％、51.67％；还检测到了其它几种异硫氰酸酯，如异硫氰酸丁酯、异硫氰酸异丁酯、异硫氰酸丁烯酯、2-苯乙基异硫氰酸酯等。

The results showed the optimal conditions of pectinase hydrolysis are amount of pectinase 0.127%,temperature 45.5 ℃,and time 3.3 h; The optimal technological parameters of alcoholic fermentation are amount of yeast 3%,temperature 28 ℃,and initial sugar content 16%; The optimal technological parameters of acetic fermentation are amount vinegar agarics 8%,temperature 32.5℃,...

以锦橙为原料研制锦橙果醋，并对锦橙果醋的酿造工艺参数等进行研究，研究结果表明，果胶酶水解的最佳条件为：加酶量0.127%、温度45.5℃、时间3.3h；酒精发酵的最优条件为：酵母接种量3%、温度28℃、初始糖度16%；醋酸发酵的最优条件为：醋母接种量8%、温度32.5℃、酒精度7%；澄清效果以壳聚糖添加量0.05%时为佳。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。