更换

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

This, in turn, of course, makes it easier to reflect its sequential character, to represent it, that is, as an irreversible stream of noninterchangeable situations, and so, in retrospect, to understand better whatever is governed by regular laws in society.

这就很容易显出其连续不断的特点，将其作为一种不可更换的情状、无可逆转的潮流加以描述，因此，在回顾中，也能更好地理解社会由通常规律支配的那些内容。

Green environmental protection , nonutility battery; Other cool hat is put into use but being less than 1 year, if adopt battery , often is comes across the pretty pass having no an electricity , if is necessary to change battery again , then it is possible use and above to cost has been estimated hundred dollor

绿色环保，不用电池；而其它风凉帽子使用不到一年，如果采用电池，费用估计上百元以上，并且经常遇到没电的尴尬，必须再更换电池才能用

On the machine to do maintenance and oilway, after cleaning, gas alarm, etc.

对全机做系统的维修后要清洗油路、气路，更换润滑油等。

The most common reoperation was polyethylene exchange because of wear or osteolysis.

最常见的再手术原因为因磨损和骨溶解导致的聚乙烯衬垫的更换。

In order to guarantee safety and stability, and to minimize costs for repair and maintenance, it is desirable to make a suitable rule determining the range of out-of-round, and to detect、remove and repair the non-roundness wheels in time. The paper analyzes wheel ovalization, which is called the second order periodic non-roundness of wheels.

为保证此方面的行车安全和稳定，提高铁路运输维护维修的经济效益，需要制定合理的依据，确定车轮非圆化的允许范围，及时对非圆化车轮进行检测、更换和镟修。

In conclusion, a patellofemoral replacement can be reised

所有病例中，原有的全聚乙烯髌骨假体均未更换。

objiective:to investigate the causes of the contamination of dialysis fluid.methods the level and species of bacteria were detected.results:the pathopoiesis bacteria and pathopoiesis bacteria in condition were identified in dialysis fluid,fluid b.offlake of soft water and preparing container.conclusions the contamination of'dialysis fluid is related with the untimely change of the filter in soft water machine and membrane of reverse osmosis,the contamination of fluid b.

目的：调查透析液和透析用水细菌污染的原因。方法：按《医院感染管理规范》的要求检测并鉴定致病菌。结果：透析液和透析用水、b液、软水口、配液容器培养出致病菌和条件致病茵。结论：血液透析液和透析用水细菌污染与软水机过滤器和反渗膜使用时间过长引起破损，未能及时更换，以及b液配制过程中细菌污染有关。

Its features includes: faster molding; larger molding area with maximum length of 49 inches; easy to replace die and sheets; demould by quacking; secondary patrix, two –way temperature control; automatic diagnosis; extra powerful cooling wind; cooling water module plate.

本机特点主要有：成型速度快；成型面积大，最长可达到49寸；更换模具、片材方便；震动脱膜；二次上膜；双向控温；自动诊断故障；特强冷风；冷水模盘。

Rasmussen nor any of the other riders in the peloton were able to make a dent on Armstrong's domination.

环法组委员主席明年将更换人选，即将退休的勒布兰克也认为，今年的比赛是最精彩的：以我这个年龄，本来应该是已经厌倦了，但我仍然能感到很刺激。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。