曲酶

By solving the quadratic regression model equation with appropriate statistic methods, the optimal conditions of cultivation time, temperature and ratio of substrate to water are 32.8 h, 31.2 ℃ and 1:1.24 respectively.

通过对二次多项回归方程求解得知，在上述自变量分别为培养时间32.8h，培养温度31.2℃，固水比1：1.24时，糖化酶的产量从最低值656.2μ/g干曲提高到1645.1μ/g干曲。

Xylan was not the major factor which affected the xylanase production. Xylose inhibited the xylanase production.

外加木聚糖进行制曲后对产酶有一定的提高，而木糖会抑制产酶，葡萄糖对产酶没有影响。

The purpose of the experiment is to realize the prokaryotic expression of β-glucosidase gene from Acetobacter xylinum ATCC 23769, which will be used to produce enzyme preparation so as to develop the efficiency of extracting resveratrol from plants. Aspergillus niger β-glucosidase gene is cloned both at DNA lever and cDNA lever, which will lay a foundation for further research about its expression in Saccharomyces cerevisiae in a secretory form with the aim of increasing resveratrol contents in wines.

本实验实现了木醋杆菌β-葡萄糖苷酶基因的原核表达，目的是为了生产粗酶制剂，用于提高从植物中提取白藜芦醇的得率；分别在DNA和cDNA水平从黑曲霉中克隆β-葡萄糖苷酶基因，为下一步在酿酒酵母中实现分泌表达以提高葡萄酒的白藜芦醇含量的研究奠定基础。

At present, most of lactases used in industry production come from Kluyveromyces, Aspergillus niger and Aspergillus oryzae.

目前，工业生产中使用的乳糖酶主要来源于乳酸克鲁维斯酵母菌、黑曲霉和米曲霉。

However, the 3 groups from medium A exhibited the highest microbial diversity and best decolorization results with 99.53% and 97.42% color removal rate of Reactive Red M-3BE and Acid Red. From them, 16 strains of fungi were isolated and primarily identified as Saprolegniaceae, Eurotiaceae, Erysiphaceae and Physodermataceae . Fungi groups from medium B and D exhibited a bit lower color removal rate of various dyes and only 3 and 2 isolates primarily classified as Saccharomycetaceae and Eurotiaceae were obtained from them. Fungi cultures in medium A and B could produce lignin peroxidase, and those in medium D could be detected higher activity of laccase. All the fungal cultures exhibited very weak activity of manganese dependant peroxidase.

来自A培养基的3组菌群显示出最好的脱色效果和最大的菌群丰富度，对50mg/L的活性红M-3BE和酸性红A溶液的脱色率最高达到99.53%和97.42%，从中分离到了16株真菌，初步鉴定分属于水霉科、曲霉科、节壶菌科和白粉菌科；而B和D培养基中所获得的菌群脱色效果稍差，从中仅得到3株和2株真菌，初步鉴定属于酵母和青霉。A、B两种培养基在各种染料存在下更易产生木质素过氧化物酶，产漆酶能力较弱，而D培养基产漆酶活性较高。

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

The sensitivity of these amylases toward pH will be greater.

温度、pH对于黑曲霉的液曲麦芽糖酶的影响。

Resistance to atracurium as a result of increased drug binding to 1-acid glycoprotein is associated with increased inducible nitric oxide synthase activity and increased nitric oxide levels in plasma.

对于阿曲库铵的阻滞是由于增加了诱导性氧化亚氮合酶活性和氧化亚氮在血浆中的水平从而使阿曲库铵与1-酸糖化蛋白的结合增加的结果。

No product except ours has been developed to specifically detoxify AFB1, the most toxic mycotoxin.

除本项目拟研发的重组黄曲霉毒素解毒酶产品，是特异性的针对毒性最强的霉菌毒素---黄曲霉毒素之外，目前没有同类产品。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。