曲酶

The results showed that temperature rise would inhibit their esterification capacity, ethyl caproate-producing capability of raw zymin of 1# high-temperature monascus improved with the prolong of the esterification time when ethanol concentration was 4 %vol and caproic acid content was 4 mL, and when ethanol concentration and caproic acid content was within proper range, caproic acid content had more effects on the formation of ethyl caproate by raw zymin than ethanol concentration.

结果表明，温度相对升高对其酯化力的形成有一定的抑制作用；高温型红曲1#酯化菌株粗酶制剂的酯化力在乙醇浓度为4 %vol、己酸含量为4 mL时，产生己酸乙酯的能力随酯化时间的延长而增强；在适宜的乙醇浓度和己酸含量范围内，己酸含量对酯化酶粗酶制剂己酸乙酯生成影响比乙醇含量的影响大。

Under the optimized conditions as follows: ratio of apple pomace and cottonseed meal 1︰1, urea content 2.6%, KH2PO4 content 0.09%, glucose content 2%, initial moisture content 62.9%, fermented at 30℃ for 60 h, 5662 and 30000 U/g of xylanase and cellulase were produced, respectively.

在最佳条件（基料棉粕和苹果渣比例为1︰1，第2氮源尿素2.6%，无机盐KH2PO4 0.09%，速效碳源葡萄糖2%，含水率62.9%，30℃培养60 h）下，分别获得了5662、30000 U/g的木聚糖酶和纤维素酶的高酶活发酵干曲。

Methods: The health adult human primary hepatocyte microsome was randomly divided into 12 groups for control and itraconazole with different concentrations in the range of clinical drug blood concentration, each group have 10 samples. Control groups were added culture fluid, itraconazole groups were added itraconazole with different concentration respectively, after cultured 30 minutes, substrates (phenacetin for CYP1A2, testosterone for CYP3A4) were added and cultured for another 40 minutes.

采用健康成人肝细胞微粒体，分为对照组和不同浓度的伊曲康唑组，共12组，每组10个样本，伊曲康唑组分别加入血药浓度范围内不同对应浓度的伊曲康唑，对照组仅加入培养液，孵育30 min后，再加入CYP450同工酶1A2和3A4的相应底物再孵育40 min。

No xylanase activity was found when the culture was carried out without xylan in the medium or with the saccharose as the only carbon source in the medium. Xylose inhibited the xylanase production.

发酵培养基中不含木聚糖时，米曲霉RIB128不产生木聚糖酶，蔗糖为底物时也不产生木聚糖酶，葡萄糖可以诱导产生少量木聚糖酶，木糖则抑制木聚糖酶的产生。

Aspergillus WQ is selected to explore the factors which affect producing enzymes. The best carbon source is crystallitic cellulose+straw powder, the best nitrogen source is peptone+yeast abstract cream, the best growing temperature is 35℃and the best initial pH is 5.5. WQ can produce the largest quantity of enzymes after being cultivated eight days.

从50℃左右牛粪自然堆肥中筛选了一株产酶量较高的曲霉WQ，对影响其产纤维素酶的因素和酶的热稳定性进行了初步研究，结果表明：液体发酵时，该菌最佳碳源是微晶纤维素+稻草粉组成的复合碳源，最佳氮源是蛋白胨+酵母膏的复合氮源，最适生长温度为35℃，最适起始pH值为5.5，在生长第8天产酶量最高。

Pachyrhizus red starter health wine was produced with pachyrhizus and rice as raw materials by semi-solid low-temperature fermentation of red starter ,saccharifying enzyme and yeast.

以地瓜、大米为原料，采用红曲、糖化酶、酵母半固态低温发酵生产地瓜红曲保健酒，确定最佳发酵工艺条件，原料配比为地瓜∶大米为13∶，红曲添加量为10%，起始pH值为5.0，加水量150%，发酵时间为8d。

The results were as followed: 1 Mutagenesis Breeding of High Tannase Activity Strains and preparing of Tannase As a original strain, Asp. niger CY was mutated by UV treatment. One strain was obtained from the mutants.

全文实验结果如下： 1 单宁酶菌种的选育和单宁酶的制备以一株产单宁酶的黑曲霉为出发菌株，进行了菌株的诱变选育，得到了一株单宁酶高产菌株。

Objective To investigate extended-spectrumβ-lactamasesand AmpCβ-lactamases and metalloβ-lactamasesproducing by gram-negative bacilli in burns.Mehtods Adopitng double-disk synergy test,modified three-dimensional extract test and the enzyme inhibitor of EDTA-Na2to detect ESBLs and AmpC and mBLA producing strains.Using K-B test to perform the susceptibility testing.Results Among348strains of gram negative bacilli,ESBLs,AmpCβ-lactamases,ESBLs combined with ApmCβ-lactamases and mBLA produc-ing strains were found in122,47,9and20strains,the incidence being35.1%,13.5%,2.6%and5.7%respec-tively.

目的 了解烧伤感染主要革兰阴性杆菌产超广谱β-内酰胺酶、AmpC酶和金属β-内酰胺酶及其对常用抗生素耐药性情况，指导烧伤医院感染合理用药方法采用双纸片协同试验检测产ESBLs株，头孢西丁三维试验检测产AmpC酶株，头孢曲松三维试验检测同时产ESBLs和AmpC酶株，依地酸二钠协同试验检测产mBLA株，K-B法进行G -杆菌药敏试验。

Vmutageniz, We gained a stain of Aspergillus niger S13 with high cellulase production, Further more we research on the optimum solid culture a wheat stem 70 % bran 30 %, nitrogen source is yeast extract 0.68%,(NH4)2SO4 0.35%, trace element 0.1%, Vogel's solution 4 %, pH3.5, temperature 30℃, cultivation time 72-96hr, With such condition, its β-glucosidas activity reached 801.18mg·glucose/g·h, CMCase activity 6262.46mg·glucose/g·h filter paper activity 20.73IU.

本文通过分离筛选和紫外诱变法，选充出一株高产纤维素酶黑曲霉菌株S13，并进行了最佳固态发酵培养条件的研究，其结果为：最佳碳源为1％碱洗麦杆粉70％，麸皮30％；另加酵母膏0.68％，(NH_4)_2SO_4 0 35％，微量元素0.1％，Vogel's母液4％，初始pH3.5培养温度30℃，培养时间为72～96 小时，在此条件下其β-葡萄糖苷酶活力达801.18mgG/h·g，CMC酶活6262.32mgG/h·g，滤纸酶活20.73IU。

Results: Carbonic anhydrase, Na+-K+-AT -Pase, Mg2+--ATPase, 5'-nucleotidase and NADPH oxidase could manifest enzyme activity on endosome membrane in the renal proximal tubules、hepatic cell of rat and human leukocytes.

结果 上述碳酸酐酶，Mg2+-ATP酶，Na+-K+-ATP酶，5'-核苷酸酶及NADPH oxidase分别在大鼠肾脏近曲小管，肝脏及人体白细胞的内吞体膜上都有酶活性存在。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。