曲酶

We investigated if the inhibition of inducible nitric oxide synthase and suppression of nitric oxide can reverse the resistance to atracurium.

我们研究是否抑制诱导性氧化亚氮合酶活性和氧化亚氮在血浆中的水平可以逆转对于阿曲库铵的阻滞。

Our results suggest that the mechanism leading to increased expression of 1-acid glycoprotein and consecutive increased protein binding of atracurium is not mediated by inducible nitric oxide synthase induction and nitric oxide expression.

我们的结论是1-酸糖化蛋白的表达和阿曲库铵与其结合的持续增加不是由诱导性氧化亚氮合酶和氧化亚氮所介导

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Objective To compare the clinical effects of letrozole and clomiphene citrate on ovulation induction.

目的 比较芳香化酶抑制剂——来曲唑和氯米芬促排卵的临床疗效。

Objective To compare the clinical effects of letrozole and clomiphene citrate on ovulation induction.

核心提示：目的比较芳香化酶抑制剂——来曲唑和氯米芬促排卵的临床疗效。

Objective To compare the clinical effects of letrozole and clomiphene citrate on ovulation induction.

目地 比较芳香化酶抑制剂――来曲唑和氯米芬促排卵地临床疗效。

Purpose:The aim is to study the optimal co reaction condition of producing high content fructooligosaccharides for immobilized cells and glucose isomerase.

目的 ：研究固定化黑曲霉细胞与葡萄糖异构酶协同反应生产高含量低聚果糖的优化条件。

The result shows that with the increase of stress time, relative water content on leaf is in a downward trend while Sedum spurium \\\"Coccineum\\\"、Sedum reflexum have a slight increase in the mid; MDA content in these plants changes very slowly except that in Sedum spurium \\\"Coccineum\\\" which continues to increase; the relative electric conductivity in the first 20d in four kinds of plants is on the rise and the value of sedum lineare and Sedum spurium \\\"Coccineum\\\" decreases in the last 5d of stress; the changes of SOD activity in four sedums are similar, which increase first and then decrease and then rise again.

结果表明：随着胁迫时间的延长，植物叶片相对含水量呈下降趋势，红叶景天和反曲景天中期值稍有上升；丙二醛除红叶景天持续上升外，其它三种植物变化缓慢；相对电导率四种植物在前20d都呈现上升趋势，胁迫最后5d时佛甲草与红叶景天值下降；SOD酶活性四种植物变化趋势相似，呈现先上升后下降再上升趋势。

Wuliangye series of wine with a unique "bag song" agent for the saccharification and fermentation, fermentation temperature, the formation of different strains, enzymes, beneficial to esterification, flavor and aroma, and the accumulation of a product's unique style; 5 food formulations, the aroma of different food and wine producing features together form a more rich, strong and comprehensive style features in the liquor came out top.

五粮液系列酒以独特的&包包曲&为糖化发酵剂，发酵的不同温度，形成不同的菌系、酶系，有利于酯化、生香和香味物质的累积，构成产品的独特风格；五粮配方，将不同粮食的香气和产酒的特点融合在一起形成了更丰富、浓厚、全面的风格特点，在浓香型白酒中独占鳌头。

METHODS: The osteoblasts were harvested from the frontal bone of 15-day SPF chicken embryos by the enzyme digestion, and treated with various mass concentrations of estrogen (0, 5, 10, 20, 100, 200, 400, 800, 2 000, 20 000 ng/L) and letrozole (0, 5, 10, 25, 50, 100, 250, 500, 1 000, 5 000 μg /L).

取15日龄SPF鸡胚，酶消化法获取鸡胚额骨成骨细胞，分别采用不同质量浓度雌激素(0，5，10，20，100，200，400，800，2 000，20 000 ng/L)和来曲唑(0，5，10，25，50，100，250，500，1 000，5 000 μg /L)处理。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。