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Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.
结果:倒置显微镜和透射电镜观察显示,经SAHA处理的细胞增殖速度显著减慢,细胞体积增大,细胞核较小,形状较为规则,核仁数量减少、体积变小,核内常染色质增多而异染色质减少,核质比例减小,细胞质内线粒体数量增多、线粒体嵴发达,高尔基体较为典型,粗糙型内质网增多,呈现出与正常上皮细胞相似的形态变化;MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用,并有明显的剂量依赖和时间依赖关系;免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达;流式细胞仪检测结果显示,SMMC-7721细胞经SAHA处理后,G0/G1期细胞明显增加,S期细胞则明显减少,细胞被阻滞于G0/G1期;RT-PCR检测结果表明,SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加,24h后更为明显。
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Methods With AT89C51 single-chip as hardware and programmed by C language, the instrument applied, the optometer adopted LCD and international standard optotype. The optotype had 14 lines of object-finders, and only one line of random characters was displayed on the screen every time.
硬件采用AT89C51型单片机,软件采用C语言编程,显示部分采用液晶显示,以国际标准视力表&E&字为视标,视标共14行,每屏显示1行,随机显示字符,视力检查距离为3m,全部程序在单片机控制下完成。
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The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.
结果:骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后,可见下领升支外侧的骨皮质上有新骨形成,新骨呈山峰状凸起,术后第28天的新生骨较低平,多孔隙,随着固定时间的延长,新骨逐渐成熟;下领升支前后向切线位X线投照显示新骨呈山峰样隆起;经测量,术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm;脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长,以及胶原纤维和骨小梁排列方向上的变化,术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示,与对照组相比较,实验侧骨膜增生明显,细胞间排列紧密,单位面积内骨膜细胞数增多,细胞核饱满;骨膜PCNA 免疫组化染色显示,与对照侧相比较,实验侧骨膜在牵张后出现了明显的增生迹象,PCNA阳性细胞分布紧密,单位面积内阳性细胞数较对照组多,靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。
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The display apparatus with the camera includes a display element in which display pixels are arranged, and an image-taking module which forms a plurality of object images through a plurality of aperture areas formed among the display pixels of the display element and photoelectrically converts the plurality of object images.
具有照相机的显示器装置具有:配置了显示像素的显示元件;通过形成在上述显示元件的上述显示像素之间的多个开口区域形成多个物体像,并对该多个物体像进行光电转换的摄像模块。
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This system can display the numbers, letters, Chinese characters. It can display the information timely and circularly, up-rollingly, down rollingly left-rollingly, right-rollingly. The lightness of the displaying information can be adjusted continuously.
本系统不仅能够实现数字、字母、汉字等预存信息的切换显示,同时还可以实现信息的定时循环、上下左右滚屏、LED显示亮度连续可调、实时时间显示、实时温度显示、无线遥控、显示屏旋转等扩展功能。
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Organic electroluminescent thin-film technology has more predominating virtues than others in the display field, such as lower power consumption , yieldingness , rapider response speed , wider viewing angle , full-color displaying with bigger area, multi-color emission et al, and it can be compatible with the lower-cost light devices which have multi-criterions and multi-technology, so it shows stronger preponderance in the flat-panel displays.
有机电致发光薄膜技术较显示技术有其突出的优点,如功耗低、易弯曲、响应速度快、视角广、可大面积显示、发光色彩齐全等,并可与现有的多种标准、技术兼容制成成本低的发光器件,因而在实现彩色平板显示方面显示出强大的生命力。
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Flash candling is a quality sorting operation which involves searching for several defects: cracks, blood spots, dirt spots, and undergrades, any one of this downgrade the egg quality.
在黑暗环境中,裂纹显示为明亮的线,血斑显示为红色的区域,污垢显示为黑点,蛋黄显示为黑色阴影,其中任何一种缺陷都会降低鸡蛋的品质。
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The digital display circuit using a figure panel instrument was designed to implement real time display of the settings and the voltage and current in the welding process.
设计的数字化显示电路,采用数字面板表进行显示。在参数预置时显示参数预置值,在焊接过程中显示焊接电压与电流。
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Payment under this credit is limited to FOB price plus any freight and insurance paid as evidenced By attested freight receipt and insurance certificate.
现在问题来了,船东不肯显示金额,因为船东到货贷到我们到客人,中间的差额多了很多,我问工行说一定要显示金额,但是我问建行说不一定要显示金额,毕竟要在工行交单,所以工行的人说不显示金额会不符合
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The company has independently developed, development, production of the products have six series, more than 400 varieties, including: wire and cable series: high-temperature compensation wire, control cables, fire-retardant cables, anti-corrosion cable, power cable, 6 ~ 110KV crosslinked power cables, computer cables, silicon rubber cables, high temperature cables, fire-resistant cables, low smoke low halogen, low smoke zero halogen cables, such as the main cable tray; Temperature Instrument Series: ITS900 in line with the International Temperature Scale and IEC standards A, B-grade Thermocouple , Explosion-proof Temperature Transmitter integration, intelligent all-input temperature transmitter; Pressure / Differential Pressure Meter Series: general pressure gauges, precision pressure gauges, pressure / differential pressure switch, the general stress / Differential Pressure Transmitter, 1151 , 3051 smart capacitive pressure / differential pressure transmitter; Level Instrument Series: glass / glass level gauge, quartz glass tube liquid level, the electric-contact level gauge, magnetic liquid level transmitter, magnetic liquid level switch, input-type hydrostatic liquid level transmitter, liquid level transmitter float electricity, etc.; Flow Meters Series: All English Intelligent Vortex Flowmeter, Turbine Flowmeter; Display Instrument Series: Intelligent Control LED digital display device, Intelligent LCD screen controlled device, Guangzhu instructions figures, such as conditioning Alarm.
公司自主研制、开发、生产的产品主要有六大系列、400多个品种,其中有:电线电缆系列:高温补偿导线、控制电缆、阻燃电缆、防腐电缆、电力电缆、6~110KV交联电力电缆、计算机电缆、硅橡胶电缆、高温电缆、耐火电缆、低烟低卤、低烟无卤电缆主电缆桥架等;温度仪表系列:符合ITS900国际温标和IEC标准的A、B级热电偶,防爆型一体化温度变送器、智能全输入温度变送器;压力/差压仪表系列:一般压力表、精密压力表、压力/差压开关、普通压力/差压变送器,1151、3051智能电容式压力/差压变送器;液位仪表系列:玻璃管/玻璃板液位计、石英玻璃管液位计、电接点液位计、磁性液位变送器、磁性液位开关、投入式静压液位变送器、电浮筒液位变送器等;流量仪表系列:全中文智能涡街流量计、涡轮流量计;显示仪表系列:智能LED数字显示调节控制仪、智能LCD界面显示调节控制仪、光柱指示数字显示调节报警仪等。
- 推荐网络例句
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In the United States, chronic alcoholism and hepatitis C are the most common ones.
在美国,慢性酒精中毒,肝炎是最常见的。
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If you have any questions, you can contact me anytime.
如果有任何问题,你可以随时联系我。
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Very pretty, but the airport looks more fascinating The other party wisecracked.
很漂亮,不过停机坪更迷人。那人俏皮地答道。