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Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5(adenovirus GDF-5,Ad-GDF-5)和空载腺病毒Ad-GFP 进行扩增并测定滴度,并以不同滴度两种病毒感染第3 代hBMSCs,干预后2 d 荧光显微镜下观察GFP 表达情况,RT-PCR 检测GDF-5 在hBMSCs中的表达。

STM now on radioThe scanning tunnelling microscope is one of the most useful tools in nanoscience.

扫描隧道显微镜是纳米科学中最有用的工具之一。

Methods:The PLGA-m-THPC were prepared by o/w multiple emulsion volatilizing method.Their general properties were observed.

采用o/w的方法制备PLGA-m-THPC纳米微球,扫描电子显微镜下检测其粒径大小。

Mycobacterium species cannot be identified by acid-fast microscopy.

l 无法在显微镜下区分分枝杆菌的菌种。

Using polarizating microscope and reflecting microscope,we have studied the features of microstructure and basic composition of general silica brick and high quality silica brick used for large scale hot air stove and the effect of impurity content and high temperature reburning on microstructure.

用偏光和反光显微镜研究了普通硅砖与大型热风炉用优质硅砖的基本组成和显微结构特征,以及杂质含量和高温重烧对显微结构影响。

Contact angle meter, scanning electron microscope, atomic force microscope and X-ray photoelectron spectroscopy are used to measure the wettability, microstructure topology and chemical compositions of polystyrene surface before and after air plasma treatment.

通过接触角测量仪、扫描电镜、原子力显微镜和X射线光电子能谱对处理前后表面润湿性、结构形貌和化学成分的观测。

Bases on infrared spectroscopy analysis,X ray diffraction analysis and scan electron microscopy,an investigation was made into the microstructure characteristics of cement asphalt emulsion mastic and concrete interface.

使用红外光谱、X衍射和扫描电子显微镜,研究了水泥乳化沥青胶浆以及混凝土界面的微观结构特征。

The structure, morphology and thermal properties of the iron nanoparticles were investigated by means of X-ray diffraction, transmission electron microscopy, oxygen bomb calorimeter and themogravimetry.

采用X射线衍射能谱、透射电子显微镜、氧弹量热计和热分析测试技术表征了纳米铁微粒的结构、形貌及热性能。

Borax carmine A dye used to stain nuclei for light microscopy.

硼砂洋 红:一种用来在光学显微镜下染细胞核的染料。

Traditional Ni coating and brush electroplated composite coating of nano-diamond/Ni are prepared respectively on 45(superscript #) steel matrix. A comparative study of the structure and performance of the two brush electroplated composite coatings is carried out by metallographic microscope, scanning electron microscope, energy dispersive spectrometry and X-ray diffraction.

以45钢为基体,制备了普通镍镀层和nano-diamond/Ni复合刷镀层,采用扫描电镜、能谱仪、X射线衍射仪和金相显微镜等设备对比研究了两种镀层的组织形貌。

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