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The composition and structure of hot-dip aluminizing samples are analyzed by using metallurgical microscope, SEM and XRD.

采用金相显微镜、扫描电镜、X射线衍射等研究手段,检测分析了浸铝镀层的成分和组织结构。

GaN nanorods are successfully synthesized on Si(111) substrates with magnetron sputtering through ammoniating Ga2O3/Co films at 950℃.X-ray diffraction, scanning electron microscopy, high-resolution transmission electron microscopy, and Fourier-transform infrared spectroscopy are used to characterize the samples.

采用磁控溅射技术先在Si衬底上制备Ga2O3/Co薄膜,然后在950℃下流动的氨气中进行氨化反应制备GaN纳米棒。应用X射线衍射、扫描电镜、傅里叶红外吸收光谱、选区电子衍射和高分辨透射电子显微镜对样品进行表征。

All the rabbits were then killed and their aortas were observed by naked eyes and light microscope.

wk后处死所有动物,在肉眼及光学显微镜下观察其主动脉斑块病变程度、主动脉壁粥样硬化的病理形态学改变。

By transmission electron microscopy and biomechanical testing, the aortas of biglycan-deficient mice showed structural abnormalities of collagen fibrils and reduced tensile strength.

通过透射光电子显微镜和生物力学监测,二聚糖缺乏小鼠显示胶原纤维结构异常和减少的可拉伸长度。

The expression levels of Bcl-2 protein were assayed by Western-blot and cell proliferation was measured by MTT method.

通过倒置荧光显微镜和流式细胞仪观察细胞的转染效率,Western印迹法检测Bcl-2蛋白表达水平,MTT法测定细胞增殖情况。

Electron microscope was used to observe the growth of capillaries.

运用电子显微镜,观察MI区微血管的变化。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分离解剖耳蜗后,用碘化丙啶染色细胞核、pholloidin染色f-actin,免疫荧光抗体分别染色单链dna、核酸内切酶g和凋亡诱导因子(apoptosis inducing factors, aif),制备耳蜗铺片,激光共聚焦显微镜下观察凋亡和坏死毛细胞内的荧光信号变化。

Five hours after the noise exposure, the cochleae were harvested. mitochondria energetic function, succinate dehydrogenase activity, in the hair cells of cochleae was evaluated with a colorimetric assay using blue tetrazolium monosodium salt. the sdh-labeled organs of corti were double stained with propidium iodide, a dna intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.

采用琥珀酸脱氢酶(succinate dehydrogenase,sdh)染色法进行耳蜗基底膜细胞线粒体染色,细胞核dna荧光染料碘化丙啶(propidium iodide,pi)双重染色耳蜗基底膜,以未受噪声暴露动物为对照,显微镜下观察噪声暴露后耳蜗基底膜核固缩和核肿胀毛细胞琥珀酸脱氢酶染色的变化。

Confocal microscopy confirmed the internalization of the drug conjugates into the tumor cells.

共聚焦显微镜证实,共轭的药物通过内摄作用进入肿瘤细胞。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下,与对照组相比,经姬姆萨染色观察,西咪替丁、尼扎替丁处理的SW480细胞,先发生皱缩,体积变小,异形性减少,细胞变圆数目增多,细胞核变小,染色变淡,核仁数量减少,部分可见染色质边集现象;细胞间连接疏松,随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后,SW480细胞全部变圆,细胞核碎裂,固缩微核形成,部分不再贴壁。

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