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Corneas and subbasal nerve fibers were examined by confocal microscopy at 1 day, 1 week, 1 month, 3 months and 6 months after the operation.

手术患者分别在术后1d、1周、1个月、3个月、6个月时行角膜共焦显微镜检查,比较两组患者术后角膜上皮下神经纤维再生的情况。

Purpose: To compare the regeneration of corneal subbasal nerve fiber bundles after laser in situ kerotomileusis and both-sides LASIK, and to provide objective proof for rationalized clinical operation.

目的:利用共聚焦显微镜观察比较准分子激光原位角膜磨镶术(Laser in situ keratomileusis,LASIK)与准分子激光双面角膜磨镶术(Both-sides LASIK ,BSL)术后角膜上皮下神经纤维的再生情况,为临床合理化手术提供客观依据。

Objective Confocal microscopy was used to compare the regeneration of corneal subbasal nerve fiber bundles after laser in situ kerotomileusis and both-sided LASIK.

目的 利用共焦显微镜观察比较准分子激光原位角膜磨镶术(laser in situ keratomileusis, LASIK)与准分子激光双面角膜磨镶术(both-sides LASIK, BSL)后角膜上皮下神经纤维的再生情况。

After changing medium, BMSCs were incubated in serum-free DMEM containing BRA and dimethyl sulphoxide for 5 hours to 6 days. MAIN OUTCOME MEASURES: Cell transfection was observed with a fluorescence microscope.

主要观察指标:荧光显微镜观察细胞转染情况,免疫细胞化学染色检测转染细胞诱导后神经元表面抗原的表达。

The first, this paper introduces synoptically that the application of FRET technology in the study of protein-protein interaction and the principle of the laser confocal scan microscope.

论文首先对荧光共振能量转移技术及其在分子生物学中的应用,以及激光共聚焦显微镜原理及应用进行了概括性介绍。

Polyester nanofiber can be prepared by electrospinning process from PET chips separately dissolving in three kinds of solvents,namely,the mixture solvent of phenol and tetrachloroethane ,trifluoroacetic acid and the mixture solvent of trifluoroace- tic acid and dichlormethane.

利用静电纺丝法制备聚酯纳米纤维,选择苯酚和四氯乙烷,三氟乙酸,三氟乙酸和二氯甲烷3种溶剂分别溶解PET切片进行静电纺丝,通过扫描电子显微镜对纤维表面形态进行观察,结果表明:三氟乙酸和二氯甲烷混合溶剂溶解PET制备的电纺纤维较好。

Preparation of trifluoroacetaldehyde and trifluoroacetic acid by trifluoroethanol catalytic oxidation;2. Polyester nanofiber can be prepared by electrospinning process from PET chips separately dissolving in three kinds of solvents,namely,the mixture solvent of phenol and tetrachloroethane, trifluoroacetic acid and the mixture solvent of trifluoroace- tic acid and dichlormethane.

利用静电纺丝法制备聚酯纳米纤维,选择苯酚和四氯乙烷,三氟乙酸,三氟乙酸和二氯甲烷3种溶剂分别溶解PET切片进行静电纺丝,通过扫描电子显微镜对纤维表面形态进行观察,结果表明:三氟乙酸和二氯甲烷混合溶剂溶解PET制备的电纺纤维较好。

The varying of expression of Cfos in different phases and different brain tissue including caudate nucleus, putamen, globus pallidus, hippocampus, nuclei ventrolaterales thalami, parvocellular reticular nucleus, and dorso lateral magnocellular nucleus were examined by immunohistochemical technique.

采用Cfos免疫组化染色,在显微镜下观察Cfos于不同时相在脑膜、脑皮质区及相关核团如尾壳核、苍白球、海马、背侧丘脑腹后外侧核、小细胞网状核、背侧旁巨细胞核的阳性细胞数。

Their thermotropic liquid crystal properties were studied through polarized optical microscopy and differential scanning calorimetry.

通过偏光显微镜和差示扫描量热法对这些化合物的热致液晶性进行了研究。

Their thermotropic liquid crystal properties were studied with polarizing optical microscopy and differential scanning calorimetry.

通过偏光显微镜和差视扫描量热法对这些化合物的热致液晶性进行了研究。

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