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GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

A detail procedure for extracting aloin from fresh aloe leaves was developed by using an obturated microwave-assisted extraction device with a pressure controlling unit.

应用密闭微波萃取装置对芦荟中的有效成分芦荟甙进行了微波萃取研究,并利用透射电子显微镜对微波萃取机理进行了初步探讨。

According to the requirements of product quality inspection, also has electronic altimeter instrument, microscopes and other sophisticated detection equipment.

根据产品质检的要求,还配有电子测高仪、显微镜等精密检测设备。

The effect of sodium carbonate on the corrosion inhibition of aluminum alloy in sodium chloride solution was investigated by SEM and electrochemical means.

应用电化学实验方法和扫描电子显微镜研究了碳酸钠在氯化钠溶液中时铝合金的缓蚀作用。

Italian anatomist who was the to use a microscope in the study of anatomy and discovered the capillary system .

马尔皮基,马尔切罗1628-1694意大利解剖学家,是第一个将显微镜用于解剖学研究的人,发现了循环系统

Italian anatomist who was the first to use a microscope in the study of anatomy and discovered the capillary system .

马尔皮基,马尔切罗1628-1694意大利解剖学家,是第一个将显微镜用于解剖学研究的人,发现了毛细血管循环系统

The structure of synthetic silicides has been investigated with the analysis of channeled low angle emergence and TEM.

用掠角沟道技术和透射电子显微镜分析了这种硅化物的结构。

A method has been developed to give topography of glycyrrhiza polysaccharides by atomic force microscopy. The results showed that glycyrrhiza polysaccharides mainly consist of glucose, arabinose, galactose, but the conclusions about the main chains are different.

用原子力显微镜对甘草多糖的微观结构进行观察,实验结果表明,甘草多糖主要由葡萄糖、阿拉伯糖和半乳糖组成。

A temporary slide mount method for precise obseration of minute structure of small size arthropod ...

本文介绍了一种在显微镜下观察小型节肢动物细微结构的临时装片方法。

Auger electron spectroscopy, scanning electron microscopy and atomic force microscopy are used to analyze component and surface morphology of the films.

用俄歇电子能谱、扫描电镜和原子力显微镜对薄膜的组成成分和表面形貌进行了分析。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。