显微镜的

The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

Its rule of change was obtained.On the basis of other studies,the flocculation kinetics and floes structure was studied. By making some assumptions, collision frequency were presented.Then the the flocculation kinetics model was established through population balance equation. Comparison of experimental data and modeling results indicate that there are the same trend between the two. So the model can predict floes number and size during flocculation of Yellow River's loess particles without using empirical parameters.The TEM and SEM were used to obsever floes in the study of floe structure.The flocculation was divided as flocculi,floc and floe aggregate.The floes structure model was established by assumption that particles position in floe accords with tetrahedron.The fractal dimension of model and experimental floes was found to be coincidentSo the model can reflect the structure of flocs formed in flocculation of Yellow River's loess particles by macromolecule flocculant at a certain extent

最后，本文还对絮凝动力学和絮体结构进行了研究，在前人研究的基础上，针对高分子絮凝黄河泥沙，对絮凝过程作了一些符合试验条件的假设，建立了颗粒碰撞频率表达式，应用了颗粒数量平衡方程，从而建立了絮凝过程的动力学生长模型，模型计算结果与试验数据对比表明，二者趋势一致，在没有使用经验参数的情况下基本上能够描述黄河泥沙絮凝过程中絮体数量和尺寸分布的变化过程；对絮体结构的研究中，应用扫描电子显微镜和透射电子显微镜对絮体进行了观察，将絮体分为絮粒、絮团和絮网三个不同的生长阶段，通过假设颗粒结合位置符西安建筑科技大学博士学位论文合正四面体，建立了絮体结构模型，计算得到的模型絮体分形维数基本与试验中的实际絮体相符，在一定程度上能够反映高分子絮凝黄河泥沙生成的絮体结构。

And some are caused because the grinding stone the grain grade is not reasonable or not polished so well.

肉眼看来光洁如镜的花岗石石材的表面，在反光显微镜下和扫描电子显微镜下可以看出石材表面都存在在磨抛加工后所留下的凹坑和条痕，这些凹坑和条痕有的是在目前的加工条件下无法克服的由矿物本身的缺陷而造成的，有的则是因为在加工过程中由于磨石粗细等级不合理或磨抛不彻底所造成的。

In this work, nanostructure samples of La1-xCaxMnO3（x=0.2、0.3、0.4、0.5）were prepared by hydrothermal-precipitation method . The structural characterization of the powder samples were done by X-ray diffraction. XRD patterns showed that all the samples ate single perovskite-type phase withoutother impurity or secondary phase. The shape and size of samples were performed on Scanning Electron Microscopy. SEM photographs investigated that temperature of hydrothermal effects the phase of samples and alkalinity of the reaction mixture by ΔTad-T curve measurement device at low magnetic fielded

本论文采用水热共沉淀退火法制备了La1-xCaxMnO3（x=0.2、0.3、0.4、0.5）系纳米结构，用X射线衍射仪确定产物为单相钙钛矿结构，无任何杂峰及第二相；用扫描电子显微镜与透射电子显微镜分析了产物的大小和形貌，发现水热温度对产物的形成起决定作用，随着温度的升高，产物由薄膜状向丝状转变，而溶液的碱度可控制产物的形貌和大小，当碱度为6.35mol/L，水热温度为240 C时，La0.6Ca0.4MnO3的主要形貌为纳米丝并有少量纳米颗粒，纳米丝平均直径为40nm；最后测量了粉末样品在低磁场（1.4T）下的磁热效应，发现水热共沉淀退火法制备样品的最大绝热温度明显高于球磨法制备样品的最大绝热温变。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Based on the combination of nanoelectrodes(including metal and glass pipets) and SECM,and the droplet three-electrode system developed previously by our group,we have investigated the charge transfer kinetics at the polarizable liquid/liquid interfac...

围绕着扫描电化学显微镜与纳米探头的结合以及扫描电化学显微镜与极化的液/液界面的结合，本文研究了加速离子在可极化的液/液界面上的转移反应机理及其动力学，并对电子在液/液界面间的转移的Marcus翻转现象做了较详细的探讨。

MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标：应用光学显微镜、电子显微镜观察脊髓形态的变化，采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌，以改良的Gale联合行为评分法对脊髓功能进行评定，结果：免疫组织化学检测显示，嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度，延缓白质神经纤维的减少，促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标：应用光学显微镜、电子显微镜观察脊髓形态的变化，采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌，以改良的Gale联合行为评分法对脊髓功能进行评定，结果：免疫组织化学检测显示，嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度，延缓白质神经纤维的减少，促进髓鞘的修复与再生。

The camera length of electron diffraction in transmisson electron microscopy is one of the main technical parameters in designing electron microscope and the electron diffraction analysis to microcrystal sample. According to Bragg law, the formula of calculating TEM electron diffraction camera length is derived from the research on the ray path of electron diffraction images in TEM and the comparison on electron diffraction with ordinary electronic diffractometer. The difference of physical significance of electron diffraction camera length between TEM and ordinary electronic diffractometer is discussed.

透射式电子显微镜(Transmisson Electron Microcopy， TEM)中的电子衍射相机长度，是电子显微镜设计和对微晶体样品进行电子衍射分析的主要技术参数之一依据布拉格定律，经对TEM中电子衍射成像光路的探讨与研究，并通过TEM与普通电子衍射仪的电子衍射的对比分析，导出了TEM电子衍射相机长度的精确计算公式，阐述了TEM和普通电子衍射仪的电子衍射相机长度所表征的物理意义的区别。

Robert Hooke described the fruiting structures of molds in 1664 but the first person to see microorganisms in any detail was the Dutch amateur microscopes of his own construction Leeuwenhoek's microscopes were extremely crude by today's standards, but by careful manipulation and focusing he was able to see organisms as well as bacteria.

Robert Hooke 于1664年就描述了霉菌的果实状结构，但是第一个真正看到微生物细微结构的是荷兰的显微镜业余制作者Leeuwenhoek，他的显微镜以今天的标准看来无疑是十分原始的，然而，经过仔细的操作和对焦，他能够看到细菌大小的生物体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。