显微镜的

Moreover, the effects of nano silicon dioxide (SiO2) particles and nano SiO2-g-PBA composited particles on the of polyoxymethylene were studied by means of mechanical properties test, scanning electron microscope and TEM.

力学性能、扫描电子显微镜及透射电子显微镜等测试表明：纳米SiO2在POM中团聚明显，而SiO2-g-PBA纳米复合粒子POM中分散均匀，导致POM/SiO2-g-PBA纳米复合材料的缺口冲击强度明显高于POM及POM/SiO复合材料，当SiO2-g-PBA纳米复合粒子的质量分数为2%时，POM/SiO2-g-PBA复合材料的冲击强度是POM的8倍多，同时拉伸强度有一定的增加。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The pollen of approximately 400 species was observed by optical microscopy. In the meantime, the pollen of Punicaceae and Aceraceae was studied by scaning electron microscopy.

在对研究区近400种植物的花粉进行光学显微镜观察研究的同时，还用电子显微镜对收集比较集中的石榴科及槭树科的花粉进行了系统的研究。

It has been found that the mat-like crystal is made of zigzag fibrillar structure oriented along the shear direction in the film. The periodical length of the zigzag fibrils is about one micron, corresponding to the width of a band in mat-like crystal.

其锯齿周期的大小平均约为1微米左右，正好与在偏光显微镜正交偏振片下所观察到的草席晶形态中一条晶带宽度的尺寸相当，因此可以认为在偏光显微镜下所看到的草席晶图像只不过是这种锯齿状微纤规则弯曲所形成周期性结构的一种光学效应而已。

In this study, different nano-hydroxyapatite particles,HAP_1(25-60nm), HAP_4(the additives is heparin, 15-50nm), HAP_5(the additives is bovine serum albumin BSA, 20-80nm) were prepared by homogeneous precipitation and used heavy-gauge hydroxyapatite as comparison,we determined amount of heparin, sialic acid ,BSA adsorbed on HAP_1,HAP_2,HAP_3 by Crystal Violet assay, Bialsche method,Bradford method respectively and analyzed the binding mechanism by infrared spectrum;After taking HAP_1,HAP_2,HAP_4,HAP_5 and RBC to co-culture in vitro,we studied RBC hemolysis test and detected RBC hematolysis rate by erythrocyte osmotic fragility test;observing the changes of morphous and locomotion of cell after coacting HAP_1,HAP_2,HAP_4,HAP_5 and RBC by light microscope and inverted phase contrast microscope;observing HAP_1,HAP_2,HAP_4,HAP_5 effecting on Ultrastructure of RBC.

本文用均匀沉淀法制备了HAP_1(25-60nm)，HAP_4(添加剂肝素，15-50nm)，HAP_5(添加剂牛血清白蛋白BSA，20-80nm)等纳米粒子，并用大尺寸的羟基磷灰石HAP_2(470-520nm)，HAP_3(1906nm)作对照，分别利用结晶紫法、Bialsche法、Bradford法研究了肝素、唾液酸、血清白蛋白在HAP_1，HAP_2，HAP_3上的吸附量，用红外光谱分析其中的结合机理；在体外将HAP_1，HAP_2，HAP4_，HAP_5与红细胞共培养，进行了红细胞溶血试验的研究，并借助红细胞渗透脆性试验检测红细胞溶血率；运用普通光学显微镜和倒置相差显微镜观察了HAP_1，HAP_2，HAP_4，HAP_5与红细胞作用后细胞形态及运动的变化：透射电镜观察了HAP_1，HAP_2，HAP_4，HAP_5对红细胞超微结构的影响。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法：将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中，分别于移植后0d，9d和14d处死受休鼠，取其脑组织，4%多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。

Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜，使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂，接著以不同SOV浓度(25， 50， 100μM)及不同时间(8， 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜，之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取，以观察位於细胞与细胞间的蛋白质，例如：N-cadherin， alpha-catenin 和p120的改变，另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

By using the "mechanical wounding"experimental system estabilished by us, and light- and electron microscopy, autoradiography and radioisotope technique, immuno-fluorescence localization, homologous clone and RACE technique, in situ mRNA hybridization and ELISA et al., we investigated the relationship between the transportation of exogenous JA and the secondary laticifer differentiation, the relationship between the producton and accumulation of endogenous JA and the laticifer differentiation from cambia, and the effect of appling exogenous jasmonates on the natural rubber production.

利用我们建立的机械伤害诱导乳管分化的实验系统，采用光学显微镜和电子显微镜技术、同位素示踪和放射自显影技术、免疫荧光定位技术、同源克隆和RACE技术、mRNA原位杂交和ELISA等技术，研究了外源茉莉酸在橡胶树体内的移动及其对维管形成层分化次生乳管的诱导效应、内源茉莉酸与乳管分化的关系以及茉莉酸类物质对天然橡胶产量的影响。

Surface relief caused by the formation of lower bainite in an Fe-C-Si-Mn alloy has been investigated by STM. It was discovered that the subunit which is the lowest structural unit observed by TEM is actually made up of sub-subunits. The surface relief due to the formation of sub-subunit is tent-shaped, and of a non-IPS type, which indicates that the sub-subunit cannot be formed by shear. The height of relief is 60-140nm, and the maximum shape deformation is ca.

用扫描隧道显微镜对Fe-C-Si-Mn合金中的下贝氏体浮突进行了观察研究发现用透射显微镜观察到的最小结构单元——亚单元是由更小的超亚单元组成的超亚单元的浮突为&帐篷&型，而非不变平面应变型，表明它不是切变形成；超亚单元的浮突高度为60-140nm，最大形状变型量约为0.23。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。