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The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示: VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性;经4 mmol/L VPA处理MUTZ1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞浆内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示:VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性;经4mmol/LVPA处理MUTZ-1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞装内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。

Lines are induced on the surface of a photosensitive glass by focused femtosecond laser transverse writing with scanning velocity in a wide range of 40-1800 μm/s. The formed lines are analyzed using scanning electron microscope and optical microscope.

利用聚焦飞秒激光的横向直写方式,采用宽的扫描速度范围(40-1800 μm/s),在光敏玻璃表面上诱导了直线结构,并扫描电子显微镜和光学显微镜分析了形成的直线,发现随着扫描速度不同,形成的直线具有三种不同的形貌。

The invention discloses a method for using light guide fiber and indium stannic oxide to make the optical scanning channel microscope probe.

本发明公开了一种用光导纤微和铟锡氧化物制作光子扫描隧道显微镜探针的方法,旨在提供一种能够制作同时具有优良的导电和透光性的光子扫描隧道显微镜探针的方法。

A study is presented concerning the three different structural levels of isotactic polypropylene.

本文应用光学显微镜,扫描和透射电子显微镜从三种不同层次的结构水平上研究了α和β两种晶型的全同立构聚丙烯的球晶和晶片形态结构,特别是应用四氧化钌染色技术直接观察到两种不同晶型聚丙烯球晶中单独分离的晶片形态。

The experimental group has various changes in the different density of potentill anserine extract. the small rat pulmonary tissue surface ofⅰgroup can see sporadic petechia of different degree; pulmonary alveolus cavity can see sporadic erythrocyte under the microscope. the small rat pulmonary tissue surface of ⅱ group is no obvious abnormality by naked eye; pulmonary alveolus cavity can see sporadic erythrocyte and thin powder color liquid under the microscope.

实验组不同浓度的蕨麻提取物对小鼠肺组织的影响不同,ⅰ组小鼠肺组织表面肉眼见不同程度的散在点状出血、显微镜下肺泡腔内见散在红细胞;ⅱ组小鼠肺组织表面肉眼无明显异常,显微镜下肺泡腔内见散在红细胞及淡伊红色液体,两组均见肺泡壁毛细血管扩张充血及毛细血管内皮细胞呈不同程度的增生肥大。

This paper gives several methods to validate these new methods by analyzing images of stage micrometer, metallurgical sample, and images that consisting of definite area in geometric graph, Several kinds of validation methods including of traceability of a quantity value, printing microstucture graph, comparing microstucture photo with standard photos, length meansurement, and area meansurement are discussed in this paper.

通过分析显微镜刻度尺图像、金相样品图像,及给定特定图形且已知面积的图像,依据ISO/IEC 17025检测和校准实验室能力的通用要求,并结合金相检验标准,阐述了金相显微镜利用新方法的方法确认,其中包括量值溯源、金相组织输出、图谱比较、长度类测量和面积类测量的确认方法。

The illumination used, so called soft x rays in the wavelength range of twenty to forty angstroms (an angstrom is one ten billionth of a meter), is also sufficiently penetrating to image intact biological cells in ma ny cases.

在许多情况下也能够穿透完整无缺的生物细胞并成像。由于使用的 X 射线的波长使软性 X 射线显微镜永远比不上电子显微镜可能具有的最高分辨力。

The surface morphologies of coal pitch spheres were characterized by scanning electron microscope and polarized light microscope. The effects of naphthalene content on the softening point of the modulated pitch, balling temperature on the spheres morphologies and the max mass ratio of pitch to the liquor were investigated.

使用扫描电子显微镜和偏光显微镜对沥青球的表面形貌进行表征,探讨了减黏剂萘含量对调制沥青软化点的影响,成球温度对球形貌的影响以及沥青/水相的最大质量比。

Electron microscope of the scheme of chart of X ray diffraction that analyses composite material, transmission and photograph of scanning electron microscope make clear: OMMT basically is with coming off condition disperses in PLA matrix, form in order to come off model give priority to, the accept rice that at the same time existence inserts a structure composite material, the affiliation of OMMT makes the drawing section of composite material ruptures to tenacity by brittleness change.

分析复合材料的X射线衍射谱图、透射电子显微镜和扫描电子显微镜照片表明:OMMT主要以剥离状态分散在PLA基体中,形成以剥离型为主、同时存在插层结构的纳米复合材料,OMMT的加入使复合材料的拉伸断面由脆性断裂向韧性断裂转变。

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