显微镜使用

This research and development effort bore its first noteworthy fruit in 1886 when Zeiss marketed the first "aphochromate" microscope objectives; this apochromatic microscope objective offered superior quality. Employing "fluorspar" elements this was the first use of crystal in an industrial optical application.

这些研究和发展的努力在1886年结出了第一个果实，蔡司公司于当年推出了"aphochromate"显微镜即复消色差透镜显微镜；此种显微镜提供了出众的品质，其中萤石成分的使用是玻璃晶体在工业光学中的首次应用。

And thaw, and cell fusion; Understanding the principles and operation procedures for autoradiograph; Mastering the skills of writing experiment reports and graphic drawing under microscope.

本课程是农业与生物学院本科学生的一门实验课程，本课程主要内容包括：掌握和了解普通光学显微镜、几种特殊显微镜和电镜的一般原理和使用方法；掌握显微镜临时标本的制备，会进行显微测量、细胞计数和染色体观察；初步观察细胞生理现象、细胞化学成分显示、染色体标本制备；掌握细胞培养、细胞冻存与复苏、细胞融合等技术的原理和方法；了解细胞放射自显影技术的原理和操作步骤；掌握实验报告的书写和镜下绘图的技能。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

Nowadays, in the low electron dose condition, the information about 3-4〓 can be recorded on the cryoEM micrograph, and the high-resolution reconstruction using the cryoEM has reached 6. 5-8〓 levels. The reconstruction can reveal the protein α helical and recognise β fold secondary structure. Secondly, it is due to the development in 3D reconstruction method, which can be more efficient to determine the orientation and center parameters. The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

The study of the structure of metals and alloys, especially by optical and electron microscopy and x-ray diffraction.

金相学，金属结构学以金属与合金结构为研究对象的学科，尤指用光学和电子显微镜使用''。

The characterization of modified surfaces were explored by contact angle measuring meter and atomic force microscopy,the tribological properties of samples in nanometer and millinewton scale were measured by friction force microscopy and CETR UMT-2 tribometer. The effects of ultraviolet radiation,sliding velocity and load on tribological properties of samples were analyzed.

在铝、钛金属薄膜和铝、镁、钛及钛合金上制备自组装分子膜，使用接触角测量仪、原子力显微镜对改性的表面进行表征，使用摩擦力显微镜和微摩擦磨损试验机对试样在纳米尺度和毫牛尺度下的摩擦学特性进行测试，分析了紫外照射、滑动速度、载荷对试样的摩擦学特性的影响。

High-speed electronics than the wavelength of visible light wave length (wave-particle duality), while the microscope resolution by its restrictions on the use of wavelength, so the resolution of electron microscopy (about 0.1 nm) is much higher than the resolution of optical microscopy (about 200 nm).

高速的电子的波长比可见光的波长短，而显微镜的分辨率受其使用的波长的限制，因此电子显微镜的分辨率（约0.1纳米）远高于光学显微镜的分辨率（约200纳米）。

Titanium plate first was cleaned by glow Discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to the free terminal NH2 groups using glutaraldehyde as a coupling agent. Surface morphologies were observed by scanning electron microscopy and atomic force microscopy.

样本以扫描式电子显微镜及原子力显微镜﹙AFM﹚观察表面形态变化，以能量分散光谱仪进行表面元素变化之定性分析，使用X光光电子能谱仪检测表面化学键结与元素变化作定性分析与半定量分析，并以胶样金免疫标示法配合使用场发射枪扫描式电子显微镜(Field Emission Scanning Electron Microscope， FEG SEM)，观察钛金属表面白蛋白连接情形作定性分析与定量分析。

Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜，使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂，接著以不同SOV浓度(25， 50， 100μM)及不同时间(8， 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜，之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取，以观察位於细胞与细胞间的蛋白质，例如：N-cadherin， alpha-catenin 和p120的改变，另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。