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Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠,融合筛选抗DR5杂交瘤细胞,制备抗DR5单抗——mDRA6;流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率;荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化;MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响;琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

The results showed that with treatment of the fixative solution added with antimonite,the free Ca2+ in pear different organs and tissues could be changed into antimonite calcium and precipitated in situ,which,after being stained,appeared as black particles under electronic microscope,whereas such particles could not be observed in the treatment without antimonite.

结果表明:在固定液中加焦锑酸钾处理进行制样,梨树不同器官或组织细胞中的自由态Caa2+,都能生成焦锑酸钙在原部位沉淀,经染色后在电子显微镜下呈现出黑色颗粒,而固定液中不加焦锑酸钾处理的则没有这种颗粒。

The results showed that the apolar fraction of the root exudates from the highly resistant cultivars could kill the pathogen. Those of the general resistant cultivars could inhibit the spore germination and hyphal growth,while the sensitive cultivars couldn't.

荧光显微镜观察结果表明:高抗枯萎病的品种根系分泌物的有机成分对病原菌孢子有致死作用,中抗品种的对病原菌孢子萌发、菌丝的生长有显著抑制作用,相反感病品种的根系分泌物则能够起促进作用。

Cathepsin D and β-tubulin marked with fluorescence were followed in distribution observed by laser scaning microscope, the distribution was from nucleus nearby to cytoplasm and apophysis, which suggested that they took part in traffic in cells.

荧光双标组织蛋白酶D及β-tubulin,共焦激光扫描显微镜下观察,可见二者伴随分布,均由开始的核周分布明显到散在分布于胞质及突起中,说明溶酶体与骨架蛋白密切相关,提示共同参与细胞内的运输。

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果:在同一标本上,不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况,二者的分布区域大致相同,神经元分化初期,较集中分布在核周附近,神经元分化成熟则分布于胞质及突起内。

Optic microscopy revealed evidence of bone apposition over the roughened implant surface comparable to that seen in other surfaces.

光学显微镜发现的在粗糙的种植体表面的骨同位证据类似于在其它表面所看到的。

A method has been developed to give topography of glycyrrhiza polysaccharides by atomic force microscopy. The results showed that glycyrrhiza polysaccharides mainly consist of glucose, arabinose, galactose, but the conclusions about the main chains are different.

用原子力显微镜对甘草多糖的微观结构进行观察,实验结果表明,甘草多糖主要由葡萄糖、阿拉伯糖和半乳糖组成。

The distribution of organic matter in sediments is heterogeneous. To study OM enrichment mechanism and interaction of organic matter with inorganic minerals in argillaceous sediments, we employed size grading, microscope, X-ray diffraction and gas chromatography to obtain information on OM and minerals.

在泥质沉积物颗粒分级的基础上,用显微镜检测、XRD和色谱分析等手段获取有机质和无机矿物的特征,探讨了二者间的相互关系以及有机质富集的差异性。

Here, we report a case of 38-year-old woman with generalized argyria. Pathologically, there were numerous minute fine brownish-black pigments fairly uniform in size, which were observed in the dermal interstitium, around the hair follicles, sebaceous glands, eccrine glands and the walls of capillaries. Darkfield microscopy showed refractile white particles.

本文报告一例发生在38岁女性的全身性银质沈著症,病理可见在真皮间质、毛囊、皮脂腺、汗腺和微血管壁周围有许多细小的棕黑色色素,暗视野显微镜下见到白色小点。

A temporary slide mount method for precise obseration of minute structure of small size arthropod ...

本文介绍了一种在显微镜下观察小型节肢动物细微结构的临时装片方法。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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