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显微镜

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MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

The reasonable structure arrangement of KBA microscope is analyzed. The theoretical basis is provided for the design and manufacture of KAB microscope.

最后分析KBA显微镜结构安排的合理性,从而为KBA显微镜设计和加工提供理论依据。

This structure can serve as a field emission device or a probe of scanning probe microscope.

该结构的材料可以用作扫描电子显微镜探针和场发射电子显微镜的针尖。

The camera length of electron diffraction in transmisson electron microscopy is one of the main technical parameters in designing electron microscope and the electron diffraction analysis to microcrystal sample. According to Bragg law, the formula of calculating TEM electron diffraction camera length is derived from the research on the ray path of electron diffraction images in TEM and the comparison on electron diffraction with ordinary electronic diffractometer. The difference of physical significance of electron diffraction camera length between TEM and ordinary electronic diffractometer is discussed.

透射式电子显微镜(Transmisson Electron Microcopy, TEM)中的电子衍射相机长度,是电子显微镜设计和对微晶体样品进行电子衍射分析的主要技术参数之一依据布拉格定律,经对TEM中电子衍射成像光路的探讨与研究,并通过TEM与普通电子衍射仪的电子衍射的对比分析,导出了TEM电子衍射相机长度的精确计算公式,阐述了TEM和普通电子衍射仪的电子衍射相机长度所表征的物理意义的区别。

In this study, we used poly L-latcide, poly DL-latcide-co-glycolide 75:25 (PLGA7525) and poly DL-latcide-co-glycolide 50:50 (PLGA5050) during citric acid inducing ammonium bicarbonate gas forming process to form porous polymer film, and rolled the porous polymer film to make nerve conduits with pores and multi-layered. Electron Spectroscopy for Chemical Analyzer and Attenuated Total Reflectance – Fourier-Transform Infrared Spectrometer were employed for determining elements' functionabilities and chemical compounds. Charge Coupled Device camera and Scanning Electron Microscope were employed for macroscopic and microscopic morphologies and structural observation. Differential Scanning Calorimetry was employed for measuring glass-transition temperature. Nano-indentation system was employed for measuring elastic modulus and hardness. Biodegradation and water absorption ratios were measured to analyze their chemical properties and SEM was employed for microscopic morphology of the tested nerve conduit.

本研究将以聚乳酸、聚乳酸-聚甘醇酸共聚合物(75:25)、聚乳酸-聚甘醇酸共聚合物(50:50)做为研究材料,以柠檬酸诱发碳酸氢铵发泡之气泡成型法制作具有相连通孔隙之高分子薄膜,再将高分子薄膜卷制成特殊多层的导管,制作具有多层孔隙之新型神经再生导管,以电子能谱化学分析及减弱式全反射-傅立叶转换红外线光谱分析进行成份分析及化学元素测定;以电荷耦合元件摄影机、扫瞄式电子显微镜进行多层孔隙型神经导管巨观及微观形态结构观察;以示差扫瞄热量量测分析玻璃转换温度、奈米三维量测弹性模数及硬度;以及进行降解性质测定,并以扫瞄式电子显微镜进行降解后之导管微观结构形态观察。

Electron microscope of the scheme of chart of X ray diffraction that analyses composite material, transmission and photograph of scanning electron microscope make clear: OMMT basically is with coming off condition disperses in PLA matrix, form in order to come off model give priority to, the accept rice that at the same time existence inserts a structure composite material, the affiliation of OMMT makes the drawing section of composite material ruptures to tenacity by brittleness change.

分析复合材料的X射线衍射谱图、透射电子显微镜和扫描电子显微镜照片表明:OMMT主要以剥离状态分散在PLA基体中,形成以剥离型为主、同时存在插层结构的纳米复合材料,OMMT的加入使复合材料的拉伸断面由脆性断裂向韧性断裂转变。

THG microscopy with circular incident polarization detects microscopic anisotropy and reveals the stacking and distribution of stromal collagen lamellae.

环形入射偏振的THG显微镜检测显微镜可见的各向异性和显示间质胶原薄层的叠加和分布。

The article introduced the measuring technique of the grain size of ceramic alpha alumina, including electronic microscope, Laue X-ray diffraction, nitrogen absorption specific surface area, infrared spectra and grinding method.

本文介绍了电子显微镜、X射线衍射、氮吸附小比表面法、红外光谱结构分析、研磨法等用于陶瓷氧化铝晶粒度测定的情况,通过对比,指出了电子显微镜能够真实地反映微观世界,是多种分析方法的基

"Crude microscope s date to the mid-15th century, but not until 1674 were the powerful microscope s of A. van Leeuwenhoek able to detect phenomena as small as protozoa."

原始的显微镜可追溯到15世纪中期,但直到1674年列文虎克才发明高倍的显微镜,能观察到小如原生动物的现象。

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