显微镜

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

The defective Langmuir-Blodgett film of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine induced by potassium oxalate(K2C2O4) and its effect on nucleation and growth of calcium oxalate monohydrate were investigated with the aid of atomic force microscopy and scanning electron microscopy.

采用扫描电子显微镜和原子力显微镜研究了经不同浓度草酸钾(K2C2O4)处理后二棕榈酰磷酯酰胆碱的缺陷LB膜及其对一水草酸钙成核和生长的影响。

The experimental group has various changes in the different density of potentill anserine extract. the small rat pulmonary tissue surface ofⅰgroup can see sporadic petechia of different degree; pulmonary alveolus cavity can see sporadic erythrocyte under the microscope. the small rat pulmonary tissue surface of ⅱ group is no obvious abnormality by naked eye; pulmonary alveolus cavity can see sporadic erythrocyte and thin powder color liquid under the microscope.

实验组不同浓度的蕨麻提取物对小鼠肺组织的影响不同，ⅰ组小鼠肺组织表面肉眼见不同程度的散在点状出血、显微镜下肺泡腔内见散在红细胞；ⅱ组小鼠肺组织表面肉眼无明显异常，显微镜下肺泡腔内见散在红细胞及淡伊红色液体，两组均见肺泡壁毛细血管扩张充血及毛细血管内皮细胞呈不同程度的增生肥大。

The morphology of frontal knobs and spine-like outgrowths in the basal part of penis of rare males of Artemia parthenogenetica of Gahai are studied by light and scanning electron microscopy.

利用体视显微镜和扫描电子显微镜，研究了中国青海尕海盐湖孤雌生殖卤虫罕见雄体执握肢前突和交接器基部刺状突的形态特征。

Based on the combination of nanoelectrodes(including metal and glass pipets) and SECM,and the droplet three-electrode system developed previously by our group,we have investigated the charge transfer kinetics at the polarizable liquid/liquid interfac...

围绕着扫描电化学显微镜与纳米探头的结合以及扫描电化学显微镜与极化的液/液界面的结合，本文研究了加速离子在可极化的液/液界面上的转移反应机理及其动力学，并对电子在液/液界面间的转移的Marcus翻转现象做了较详细的探讨。

Surface relief caused by the formation of lower bainite in an Fe-C-Si-Mn alloy has been investigated by STM. It was discovered that the subunit which is the lowest structural unit observed by TEM is actually made up of sub-subunits. The surface relief due to the formation of sub-subunit is tent-shaped, and of a non-IPS type, which indicates that the sub-subunit cannot be formed by shear. The height of relief is 60-140nm, and the maximum shape deformation is ca.

用扫描隧道显微镜对Fe-C-Si-Mn合金中的下贝氏体浮突进行了观察研究发现用透射显微镜观察到的最小结构单元——亚单元是由更小的超亚单元组成的超亚单元的浮突为&帐篷&型，而非不变平面应变型，表明它不是切变形成；超亚单元的浮突高度为60-140nm，最大形状变型量约为0.23。

This paper gives several methods to validate these new methods by analyzing images of stage micrometer, metallurgical sample, and images that consisting of definite area in geometric graph, Several kinds of validation methods including of traceability of a quantity value, printing microstucture graph, comparing microstucture photo with standard photos, length meansurement, and area meansurement are discussed in this paper.

通过分析显微镜刻度尺图像、金相样品图像，及给定特定图形且已知面积的图像，依据ISO/IEC 17025检测和校准实验室能力的通用要求，并结合金相检验标准，阐述了金相显微镜利用新方法的方法确认，其中包括量值溯源、金相组织输出、图谱比较、长度类测量和面积类测量的确认方法。

Was authorized by JAPAN OLYMPUS Corporation as the sole agent of OLYMPUS industrial Microscope in South China .

州元升科技有限公司是经日本OLYMPUS Corporation正式授权的工业显微镜中国华南总代理商，负责奥林巴斯工业显微镜及相关工业产品在中国华南地区的营业活动。

Because of the wavelength of the x rays used, soft x ray microscopes will never match the highest resolution possible with electron microscopes.

由于使用的 X射线的波长使软性 X射线显微镜永远比不上电子显微镜可能具有的最高分辨力。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。