显微镜

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

Morphologic change was observed with photomicroscopy and fluorescent microscopy.

普通光学显微镜及荧光显微镜观察榄香烯哌嗪对HeLa细胞形态的影响，利用流式细胞仪检测HeLa细胞凋亡及细胞周期变化情况。

Leaf-epidermis cells and stomatal apparatuses of Polygonatum cyrtonema at its different developmental stages were examined by light and scanning electron microscopy.

通过光学显微镜和电子扫描显微镜，对多花黄精不同发育时期的叶表皮细胞及其气孔器进行了研究。

Methods Acanthamoeba polyphaga and Legionella pneumophila were co-cultured under laboratory condition. At consecutive time points during the culture, smears of the cultured products were made on glass slides for staining purposes. Different types of stainings including Gram′s staining, Gimenez staining, Giemsa staining and immunofluorescence were used to determine the best method for the identification of amoebal pathogens.

方法嗜肺军团菌与多噬棘阿米巴共培养，取不同时点的共培养物制作涂片，采用革兰氏染色、吉曼尼兹染色、姬姆萨染色、免疫荧光染色等多种方法，用光学显微镜及荧光显微镜观察鉴别阿米巴滋养体与其胞内嗜肺军团菌，并比较这些染色方法的效果。

The spore morphology of 9 species, belonging to 6 genera of Polypodiaceae distributing in Guangxi of China was studied and compared under LM and SEM.

首次利用光学显微镜和扫描电子显微镜对广西产水龙骨科6属9种植物的孢子形态进行了观察研究。

The spores of 8 species and 1 variety belonging to 4 genera of Polypodiaceae subfam. Polypodioideae in Yunnan, China were examined under LM and SEM. Of the species it is the first time to study the spores of Metapolypodium manmeiense and Polypodiastrum mengtzeense under SEM. The results show that the spores of this subfam.

利用光学显微镜和扫描电子显微镜对云南产水龙骨科水龙骨亚科4属8种1变种植物的孢子进行了详细观察，其中篦齿蕨Metapolypodium manmeiense以及扫描电镜下观察的蒙自拟水龙骨Polypodiastrum mengtzeense为首次报道。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The mice eyes were enueleated and plastic-embedded and the anterior chamber angle were sagittally sectioned in 3 μm thickness. The morphology and structure of the eye tissues was studied by light microscopy and transmission electron microscopy.

通过塑料包埋切片技术，用光学显微镜和透射电子显微镜观察两组小鼠眼前房角组织的形态和超微结构。

The ultrastructure of spermatozoa of Takifugu xanthopterus was observed by means of scanning electron microscopy and transmission electron microscopy.

在透射电子显微镜及环境扫描电子显微镜下观察黄鳍东方鲀精子的超微结构。

The sporangia development of halophytophthora kandeliae were observed by light microscope and electron microscope in this study.

本研究将以电子显微镜搭配光学显微镜，详细观察halophytophthora kandeliae，并加以了解其游动孢囊之释孢机制。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。