显微镜

By dehydrochlorination reaction the poly[(2-methoxyl-5-octyloxy)phenylene vinylene] is synthesized and the material"s structure is characterized by nuclear magnetic resonance spectroscopy, infrared absorption spectrum, ultraviolet and visible spectrophotometry. At the meantime, the fluorescence spectrum is measured and the mechanism of fluorescence occurring is analyzed. Thermal Gravimetric Analyzer"s results show that the MO-PPV"s thermal stability is fine. Also the experiment of resolvable property indicates that the MO-PPV can well dissolve in chloroform. Through analyzing the film"s surface by Metaloscope and Atomic Force Microscope, the good filming is found.

通过脱氯缩合法合成了聚(2-甲氧基-5-辛氧基)对苯乙炔，并分别用核磁共振波谱法、红外吸收光谱法、紫外-可见分光光度法等对其进行了结构表征；测量了样品的荧光光谱，分析了荧光产生的机理；热失重仪分析了材料的热稳定性，研究表明MO-PPV材料具有良好的热稳定性；溶解性实验发现材料在氯仿中溶解性较好；金相显微镜和原子力显微镜研究了薄膜的表面形貌，发现该材料的成膜性良好；材料的导电性研究表明，固体材料在掺杂下才可以表现出导电特性，而配成溶液的材料导电性因溶剂的不同而不同，并随溶解时间的长短发生变化。

Separately in the optical microscope and electron microscope observation of the growth plate of rats in each group metaphyseal tibia morphological changes of the organization.

分别在光学显微镜和电子显微镜下观察各组仔鼠出生后20天胫骨干骺端生长板的组织形态学改变。

Leaf epidermal morphology of 26 species (including 1 subspecies and 2 varieties) of Lasianthus and 6 species of its related 4 genera Morinda, Psychotria, Saprosma and Urophyllum, were investigated under both light microscope and scanning electron microscope.

利用光学显微镜和电子扫描显微镜研究了亚洲茜草科粗叶木属植物26种（包括1个亚种和2个变种）和相关4属即巴戟天属、九节属、染木属和尖叶木属6种植物的叶表皮微形态特征。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

DCHF is a nonfluorescent compound that can beoxidized to 2', 7'-dichlorofluorescin, a fluorescent product, by ROS in cells,which can be monitored by fluorescent microscope or confocal laser scanningmicroscope.

DCHF进入细胞后被ROS夺取二氢后氧化成二氯荧光素，用荧光显微镜和激光共聚焦显微镜观察DCF的荧光强度，表示ROS生成量的多少。

Based on the development of key techniques,such as fabrication of optical fiber probe and probe-sample distance nonoptical control, ete,we have built a scanning shear force near-field optical microscope ,which can be combined with an inverted optical microscope.

在发展光纤探针制备和探针与样品近场间距非光学控制等关键技术基础上，我们研制成能与倒置光学显微镜联合使用的扫描切变力/近场光学显微镜，并具有反射和透射等工作模式以及能在溶液环境中工作。

Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%, 86.8%, 90.5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84.3%, 88.1% and 83.3%, 87.9% respectively.

结果 观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%，与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%，与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%，流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。

Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜，使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂，接著以不同SOV浓度(25， 50， 100μM)及不同时间(8， 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜，之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取，以观察位於细胞与细胞间的蛋白质，例如：N-cadherin， alpha-catenin 和p120的改变，另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞。

From two elevation altitude habitats of 3600 m, 2500 m at cold regions were analyzed and compared by means of light and scanning electron microscope using a new embedding method with PEG and Epon-812. Our results indicated that Papaver croceum Ldb.

采自乌鲁木齐河源区海拔3600m、2500m两种海拔高度，采用PEG和Epon-812两次包埋方法，在光学显微镜和扫描电子显微镜下叶片剖面结构进行了观察。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。